7
8.3. Protective tip cone
fi
lters
The tip cone design of the Biohit
m
LINE pipettes (>10
μ
l) allows the
use of Biohit Safe-Cone
fi
lters in the tip cones. These removable
fi
lters prevent liquids and aerosols from entering the pipette and,
thus, protects the pipette from contamination and damage.
The Safe-Cone
fi
lters are available as Standard or Plus versions. It
is recommended to use the Standard
fi
lter for general applications
and the Plus
fi
lter for more demanding applications such as cell
culture, bacterial and virological work and molecular biology.
Filters need to be changed regularly. The interval for
fi
lter changing
is application dependant but the recommendation is to change the
fi
lter daily (after 50 - 250 pipetting cycles) and always in case of
over-aspiration. (See Chapter 2 for ordering information.)
The Safe-Cone
fi
lters can be removed with the unique
fi
lter ejector
without touching the
fi
lters by hand. Remove the operating button
cap and eject the
fi
lter by pressing the operating button down
(Fig. 7). Make sure that the
fi
lter is disposed into a suitable waste
container. Clean the tip cone if needed and put the new
fi
lter in
place.
Fig. 7.
9. PIPETTING
TECHNIQUES
Two basic pipetting techniques, forward and reverse pipetting,
are associated with
m
LINE pipettes.
Forward pipetting
is the
most common used pipetting technique. The technique employs
the blow-out function ensuring complete delivery of the liquid.
Reverse pipetting
is recommended for highly viscous, biological
or foaming liquids, or very small volumes of liquid. A selected
volume plus an excess is aspirated into the tip. The delivery is done
without blow-out, and, thus, the excess volume remains in the tip.
The reverse technique also facilitates the
repeated delivery
of the
same volume. If reverse pipetting technique is used the pipette
might need recalibration.
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