40
OPERATION:
(Con’t)
HINTS ON USING THE FYRITE: (Con’t)
Inspect the Fyrite filter in the clear plastic casing between the hoses for
any contamination or growth. Change it if necessary.
CAUTION:
MAKE SURE THE FILTER IS MOIST BEFORE YOU TAKE YOUR
READING. A MOIST FILTER IS A MUST FOR AN ACCURATE READING.
To moisten the filter, remove the end of the short hose without the
squeeze bulb that connects to the Incubator. Add several drops of distilled
water to the filter, replace the hose and squeeze the bulb. Release the bulb and
the water will be drawn into the filter. Squeeze the bulb and quickly release it to
remove any excess water. You will see this water (if any) come out of the end of
hose that is closest to the squeeze bulb. This is the cupped end that is
depressed onto the Fyrite.
TAKING A FYRITE READING:
It is recommended that Fyrite CO
2
and independent temperature
tests be performed at intervals to be determined individually or as dictated
by established protocol. Some laboratories conducting critical work may
want to record CO
2
and temperature readings on a daily basis.
CAUTION:
THE INCUBATOR MUST BE STABLE.
First, clear the Fyrite of any residue CO
2
. Simply depress the plunger on
the head or the top of the Fyrite taking care not to cover the hole—this
introduces fresh ambient air into the Fyrite chamber. Release the plunger and
turn the Fyrite upside down, holding it at a 45-degree angle until most of the
bubbles surface and the fluid has filled the head. Even the smallest bubbles
may contain CO
2
, so that it is important to let most of the smaller bubbles surface.
Now turn it right side up and hold it at 45 degrees. Allow the fluid to fill the
Fyrite and depress the plunger again—this clears the Fyrite of any CO
2
that
might cause a false reading.
Next, attach the filter side of the hose to the Incubator's CO
2
sample port.
Remove the brass tube, if present, from the Fyrite hose, as it is not needed and
attach the hose to the CO
2
sample port. The brass tube was once used with
older Incubators that utilized a hole in the Incubator wall or door to measure
CO
2
but this method proved to cause contamination problems.
LOOK AT YOUR FYRITE GAUGE AND ADJUST THE ZERO IF
NECESSARY.
Take the squeeze bulb end of the hose and with the hole of the cupped
end facing down, place it onto the plunger of the Fyrite and hold it firmly in
position.
IMPORTANT:
ONCE YOU HAVE DEPRESSED THE PLUNGER OF THE
FYRITE, IT IS CRITICAL THAT IT NOT BE RELEASED DURING THE
FOLLOWING PROCEDURE.
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