Amersham Pharmacia Biotech AKTA P-920, Руководство пользователя

Страница: 25 / 48

4.6 Replacing the piston seal
If there are signs of leakage between piston seal and glass cylinder,
replace the piston seal of the leaking pump cylinder.
A seal leakage will gradually fill up the rinsing chamber completely,
causing excess fluid to leak out around the rinsing tubing connections.
Note: Before disassembling the pump mechanism, move all input
buffer bottles to below the level of the pump pistons to prevent
siphoning.
Note: Always replace the piston seals on both piston heads of a
pump module at the same time.
Required spare parts and tools
Seal kit containing (see Reference information D for code numbers):
• Piston seal kit containing two piston seals,two gaskets and two wipers.
• M6 wrench (included in ÄKTA
FPLC
tool kit).
• 2.5 mm Allen key (included in ÄKTA
FPLC
tool kit).
• U-wrench NV 5.5 (included in ÄKTA
FPLC
tool kit).
Note: After new sealings have been installed, check the pump for
leakage. See section 4.5 Testing leakage.
Replacement instruction
1 Use operating menu Setup Pulsecomp. to check that pulse
compensation is off, see Section B.2.6. If not, select off.
Otherwise, adjustment is not possible.
2 Start the pump and run until the piston head reaches the middle
of its cylinder. Stop the pump by setting it to
END
work mode.
Run 1.00ml/min
End Hold Pause
Setup Pulsecomp.
(on) on off
CAUTION! Read the following instructions carefully. Avoid
fitting the individual parts of the pump cylinder assembly
incorrectly. Ensure that the orientation of each part is correct
before continuing with the next instruction.
CAUTION! Do not disassemble the pump mechanism unless
there is good reason to believe that the seal is leaking. Always
ensure that sufficient spare components are available before
attempting to replace the piston seal. We do not recommend
refitting a used piston seal after removal.
Pump P-920
User Manual 18-1125-54 Edition AB
19
Maintenance
4
18-1125-54_ab.qxd 1998-10-22 12:49 Sida 19 (1,1)