Amersham Pharmacia Biotech AKTA P-920, Руководство пользователя

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Pump wash operation
1 Immerse the inlet filters in a wash solution. Select operating menu
Pump Wash and press
OK
.
2 When the display shows
Pump Wash A
,
B
and
B&A
, select
B&A
and press
OK
.
3 First pump B and then pump A is washed in sequence. The pump
wash is performed with 20 ml/min for seven strokes each.
In UNICORN, select instruction
PumpWash
in
System Control:Manual:Pump
. In the
Parameters
window set
PumpA
and
PumpB
. Click on
Execute.
3.9 Changing eluent
Note! When changing from one eluent to another, it is extremely
important that the two eluents are totally miscible with one another.
If the two eluents are immiscible, the pump should be washed first
with an intermediate liquid that is miscible with both eluents. Failure
to do this will disrupt the flow from the pump.
When changing from a salt-containing buffer to an organic solvent,
use water as the intermediate liquid to prevent precipitation.
1 Stop the pump by setting it in
Pause
mode.
2 If not carried out from UNICORN, use a bypass tubing to
replace the column.
3 Transfer the inlet tubing into the new eluent or into the
intermediate liquid.
4 Perform a pump wash.
5 If an intermediate liquid is being used, transfer the inlet tubing
into the final eluent and repeat step 4 with the new eluent.
In UNICORN, select instruction
PumpWash
in
System Control:Manual:Pump
. In the
Parameters
window set
PumpA
and
PumpB
. Click on
Execute.
Note: When high buffer purity is important, use an intermediate
step. This avoids remains of the previous buffer in the inlet filter
diffusing out in the new buffer.
CAUTION! To prevent precipitation of crystals when changing
from a salt-containing buffer to organic solvent, always wash the
system with water as the intermediate liquid.
Washing
Please wait......
Pump Wash 20 ml/min
A B B&A
Pump Wash 20 ml/min
Pump P-920
User Manual 18-1125-54 Edition AB
14
3
Operation
18-1125-54_ab.qxd 1998-10-22 12:49 Sida 14 (1,1)