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Amersham Pharmacia Biotech AKTA P-920, Руководство пользователя

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Trademarks

ÄKTA, FPLC and UNICORN are trademarks of
Amersham Pharmacia Biotech Limited or its
subsidiaries.

Amersham is a trademark of Nycomed Amersham plc.
Pharmacia and Drop Design are trademarks of
Pharmacia & Upjohn Inc.

Terms and Conditions of Sale

All goods and services are sold subject to the terms
and conditions of sale of the company within the
Amersham Pharmacia Biotech group which supplies
them.  A copy of these terms and conditions of sale is
available on request.

Adresses

Amersham Pharmacia Biotech UK Limited
Amersham Place  
Little Chalfont  
Buckinghamshire 
England HP7 9NA

Amersham Pharmacia Biotech AB
SE-751 84  Uppsala  
Sweden

Amersham Pharmacia Biotech Inc
800 Centennial Avenue   
PO Box 1327   
Piscataway   
NJ 08855 USA

© Amersham Pharmacia Biotech AB 1998 

– All rights reserved

Important user information

Meaning: Consult the instruction manual to avoid
personal injury or damage to the product or other
equipment.

WARNING!
The Warning sign is used to call attention to the
necessity to follow an instruction in detail to avoid
personal injury. Be sure not to proceed until the
instructions are clearly understood and all stated
conditions are met.

CAUTION!
The Caution sign is used to call attention to
instructions or conditions that shall be followed to
avoid damage to the product or other equipment. Be
sure not to proceed until the instructions are clearly
understood and all stated conditions are met.

Note
The Note sign is used to indicate information
important for trouble-free or optimal use of the
product.

Should you have any comments on this instruction, we
will be pleased to receive them at:

Amersham Pharmacia Biotech
SE–751 84 Uppsala
Sweden

!

18-1125-54_ab.qxd  1998-10-22 12:49  Sida 2 (1,1)

Содержание AKTA P-920

Страница 1: ...User Manual 18 1125 54 Pump P 920 18 1125 54_ab qxd 1998 10 22 12 49 Sida 1 1 1...

Страница 2: ...ech AB 1998 All rights reserved Important user information Meaning Consult the instruction manual to avoid personal injury or damage to the product or other equipment WARNING The Warning sign is used...

Страница 3: ...nd settings 9 3 3 Menu overview 10 3 4 Starting and stopping the pump 11 3 5 Setting the flow rate and starting the pump 12 3 6 Setting concentration B 12 3 7 Running a simple gradient 13 3 8 Ending t...

Страница 4: ...time 31 B 1 4 Checking service mode 31 B 2 Setup menu 32 B 2 1 Set pressure limit 32 B 2 2 Set wash pressure limit 32 B 2 3 Set pressure unit 32 B 2 4 Set gradient base 32 B 2 5 Set mixer operation 32...

Страница 5: ...part sections 1 5 and a reference part sections A D Sections 1 5 contain the necessary information for installing operating and maintaining the instrument Contents Pump P 920 User Manual 18 1125 54 E...

Страница 6: ...Contents Pump P 920 User Manual 18 1125 54 Edition AB iv 18 1125 54_ab qxd 1998 10 22 12 49 Sida iv 1 1...

Страница 7: ...hy work It is utilised as system pump in KTA FPLC chromatography systems or is used as a stand alone unit The flow rate is set locally with a front dial or from a PC running UNICORN 3 0 or higher Pump...

Страница 8: ...can be connected to the pump When P 920 is connected to UNICORN the mixer can be controlled on off auto from UNICORN When P 920 is run as a stand alone unit the mixer is operated via the local user in...

Страница 9: ...iquid to spray out This is especially dangerous if hazardous chemicals are being used Connect the tubing by first inserting the tubing fully then tightening the connector fingertight WARNING NaOH is i...

Страница 10: ...led on a stable laboratory bench or in an KTAdesign chromatography system To ensure correct ventilation a free space of 0 1 m is required behind and in front of the module Place the module directly on...

Страница 11: ...or higher via UniNet 1 cables 1 Connect two UniNet 1 cables to the UniNet 1 connectors The module can be connected in series anywhere in the chain between the PC and the termination plug The UniNet 1...

Страница 12: ...outlet tubing PEEK tubing i d 0 50 mm o d 1 16 has tubing connectors attached at both ends The inlet tubings A1 B1 teflon tubing i d 1 6 mm o d 1 8 have tubing connectors attached at one end and inle...

Страница 13: ...r 2 Switch the mains power switch on the front panel to ON A selftest is carried out Note When the pump is run for the first time after replacing a sealing friction between the glass cylinder and the...

Страница 14: ...ure the power switch is in OFF position Loosen the two screws Mount the attached mounting bracket with the two screws Use one of the mounting rails Pump P 920 User Manual 18 1125 54 Edition AB 8 2 Ins...

Страница 15: ...selection dial clockwise or counter clockwise When the required menu is visible the menu or selection is accepted by pressing the OK button Menu selection OK button ESC button Go down one menu Return...

Страница 16: ...peatedly Setting flow rate in ml min Setting concentration and gradient values Wash program selection Individual pump cylinder assemblies or a complete pump wash can be selected Pump synchronisation s...

Страница 17: ...menu displayed will depend on the current mode see below When a new mode is selected the appearance of the menu will change Pressing OK in a work mode changing display selects the underlined work mode...

Страница 18: ...ss OK 2 Set the desired value and press OK 3 Go to the work mode menu select Run and press OK 4 To change the flow rate while the pump is running repeat steps 1 and 2 5 To stop the pump go to the work...

Страница 19: ...dient starts immediately 5 At the end of the set gradient time the pump continues to run at the target concentration In UNICORN select instruction Gradient in System Control Manual Pump In the Paramet...

Страница 20: ...r as the intermediate liquid to prevent precipitation 1 Stop the pump by setting it in Pause mode 2 If not carried out from UNICORN use a bypass tubing to replace the column 3 Transfer the inlet tubin...

Страница 21: ...d it automatically restarts after performing a selftest when power is restored All values under the Setup menu are retained Other operating values e g flow rate are reset Pump P 920 User Manual 18 112...

Страница 22: ...aning Perform a pump wash using a cleaning or sanitising agent The standard recommendation is to use 1 M NaOH and then wash out with buffer or distilled water WARNING NaOH is injurious to health Avoid...

Страница 23: ...ld be changed monthly Use a 20 solution of ethanol distilled water 1 Remove the rinsing tubing between the glass cylinders for both the A and B pump 2 Select 50 B and run the pump at 20 ml min to flus...

Страница 24: ...of 0 2 ml min 6 Start the pump and let it run until the pressure limit is reached and the pump stops 7 Check the pressure reading for two minutes The pressure must not decrease more than 0 45 MPa dur...

Страница 25: ...e been installed check the pump for leakage See section 4 5 Testing leakage Replacement instruction 1 Use operating menu Setup Pulsecomp to check that pulse compensation is off see Section B 2 6 If no...

Страница 26: ...ul not to damage the piston head during this operation Fit the new seal by hand 11 The wiper is removed by pressing it out from the rod Press a new wiper onto the rod The hole on the wiper should poin...

Страница 27: ...iston rod slips into the hole in the driving arm Continue to run the pump until the 6 port pump valve switches Just as the valve switches stop the pump 21 Engage the piston rod in the driving arm by t...

Страница 28: ...and that the pressure sensor gives no indication that the back pressure has increased The probable cause is that one or more channels are blocked Identify the relevant pump cylinder assembly by obser...

Страница 29: ...ervice for advice No text on the front display 1 Check that the mains cable is connected and the power switch is in ON position I 2 Check the mains power supply 3 Check the mains inlet fuse Erratic fl...

Страница 30: ...6 Replacing the piston seal Replace if necessary 2 If the glass cylinder walls are scratched check the piston seal Ensure that the piston rinsing system is always used especially when working with aq...

Страница 31: ...leakage 4 Check for air in the pump 1 Switch off the module and let it cool 2 Clean or clear the air inlets 3 Switch on the module and check that the fan is running 1 Call service 84 ERROR endsensors...

Страница 32: ...greater than zero Reset value 3636 Value must be greater than 0 3620 Error hardware notify instr suppor 3610 Discon pressure then set analog V 3605 Low lim hi lim Hysteresis 3600 Low lim hi lim 3624 E...

Страница 33: ...ning indicator in Check menu see section B 1 1 together with the pressure trace the pump cylinder assembly which is functioning abnormally can be identified There can be several causes of an abnormal...

Страница 34: ...0 1 V Pressure signal to chart recorder UniNet 2 Connection to Mixer M 925 and UniNet 2 network UniNet 1 Connection to UniNet 1 network Mains inlet with fuse Supply voltage grounded Mains outlets Sup...

Страница 35: ...ision glass cylinders The pistons are driven so that one piston draws in solvent while the other expel solvent The change of piston direction is controlled by opto electronic switches at the end posit...

Страница 36: ...ds up to the set limit the pump automatically shuts down and an alarm is given A 6 Protective covers Pump P 920 is equipped with covers to protect both the user and the pump from accidental damage A p...

Страница 37: ...tion relevant to the module can be checked Information may not be available in all menus 1 Select operating menu Check Service Mode and press OK 2 The service telephone number1 is displayed Press OK 3...

Страница 38: ...and an error message Wash overpressure is shown This message has to be confirmed by pressing OK 1 Select sub menu Setup Wash Press Lim press OK 2 Set the value press OK B 2 3 Set pressure unit Sets th...

Страница 39: ...unit number is the identification the pump has on the UniNet 1 communication link It should correspond to the number set in UNICORN for the pump The number should be set to 0 if one pump is used If m...

Страница 40: ...the Alarm Timer menu which now shows the set alarm time or count down time as BuzzerHH MM SS 6 When the alarm time is due or the count down timer reaches 00 00 00 an alert display is shown and the mo...

Страница 41: ...nce Buzzer Test Set Alarm 12 32 21 0 00 00 00 Set Timer 0 00 00 00 Set Clock 00 36 53 00 36 53 Alarm Timer off Buzzer 05 15 00 Check Piston Status A Left compressing B Right compressing Pump Wash 20ml...

Страница 42: ...ml Inlet tubing Tubing connector for 1 8 o d tubing Outlet tubing Fingertight connector 1 16 o d Fuse specification T 6 3 AL Control Stand alone or from a PC running UNICORN version 3 0 or higher via...

Страница 43: ...or the module when it is used in laboratory locations used in the same state as it was delivered from Amersham Pharmacia Biotech except for alterations described in the User Manual used as a stand alo...

Страница 44: ...PEEK 6 A 18 1112 57 Fingertight connector 1 16 for PEEK tubing o d 1 16 10 A 18 1112 55 Union 1 16 male 1 16 male for 1 16 o d tubing PEEK 10 A 18 1120 92 Stop plug 5 16 PEEK 5 A 18 1112 50 Stop plug...

Страница 45: ...18 1125 54_ab qxd 1998 10 22 12 49 Sida 39 1 1...

Страница 46: ...November 1998 18 1125 54_ab qxd 1998 10 29 16 45 Sida 40 1 1...

Страница 47: ...18 1125 54_ab qxd 1998 10 29 16 45 Sida 41 1 1...

Страница 48: ...lf test is performed The version number is shown for two seconds 3 The main operating menu is shown 4 Set the flow rate 5 Start the pump by setting it in RUN mode 6 The main operating menu is shown 7...

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