Amersham Pharmacia Biotech AKTA P-920, Руководство пользователя

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Pump P-920
User Manual 18-1125-54 Edition AB
13
Operation
3
3.7 Running a simple gradient
Gradients can be run in time or volume base. The default is time
base. To change base, see B.2.4 in section Reference information.
The gradient is run from current
concentration to target concentration,
over a set time or volume. The
gradient can be set in any operating
mode.
1 Select operating menu Set Conc./Gradient and press
OK
.
2 When the display shows Set Concentration B, turn the dial to
select operating menu Set Gradient Length and press
OK
.
3 Set the desired value and press
OK
.
4 Turn the dial to select operating menu Set Gradient Target and
press
OK
. Set the desired gradient target value in %B and press
OK
. If the pump is in work mode
Run
, the gradient starts
immediately.
5 At the end of the set gradient time, the pump continues to run at
the target concentration.
In UNICORN, select instruction
Gradient
in
System Control:Manual:Pump
. In the
Parameters
window set
Target
and
Length
. Click on
Execute.
3.8 Ending the run and storage
Overnight storage: The pump can be left filled with a buffer.
Note: If buffers or water are stored at room temperature, bacterial
growth may occur.
If no further runs are planned, the pump should be washed
immediately with pure eluent. If aqueous buffers have been used,
washing with pure distilled water is particularly important to
prevent salt precipitation.
Weekend and long term storage: Wash the pump with water and
then fill it with 20% ethanol.
Set Gradient Target
(0.0%B) 50.0
Set Gradient Length
(0.0min) 8.0
Set Conc./Gradient
(0.0%B)
0%
100%
Target
concentration
Current
concentration
Start of
gradient
Gradient length
18-1125-54_ab.qxd 1998-10-22 12:49 Sida 13 (1,1)