Amersham Pharmacia Biotech AKTA P-920, Руководство пользователя

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4.3 General care
Inspect the pump daily for eluent leaks.
If, at any time, air bubbles are trapped in a cylinder assembly,
purge the pump by manually changing the direction of the piston
when the piston reaches its end position. Change direction several
times until the air bubbles are removed.
General recommendations for all eluents
It is essential that all liquids passing through the pump are clean,
pure and degassed. Impure or dirty eluents will not only cause
baseline noise and drift, but also block the channels in the 6-port
pump valves, shortening their lifetime. Degassing prevents formation
of air bubbles, which can cause baseline noise, drift and
deteriorating gradient formation.
Additional recommendations for aqueous eluents
After running with an aqueous eluent, the pump should always be
thoroughly washed with pure, distilled water to prevent salt
precipitation.
4.4 Changing rinsing solution
The rinsing solution should be changed monthly. Use a 20%
solution of ethanol/distilled water.
1 Remove the rinsing tubing between the glass cylinders for both
the A and B pump.
2 Select 50%B and run the pump at 20 ml/min to flush out the
used rinsing solution. Allow the pump to perform a couple of
strokes to remove as much rinsing solution as possible from the
glass cylinders.
3 Stop the pump when the pistons are at the middle of the glass
cylinders.
4 Refit the rinsing tubing to the lower cylinders in each pump
module.
5 Add fresh rinsing solution to the lower cylinders by submerging
the rinsing tubing open ends in rinsing solution and starting the
pump. Continue to run until approximately half of the cylinder
chambers are filled. Stop the pump.
6 Refit the rinsing tubing to the upper cylinders.
7 Run the pump and allow a couple of strokes to wash out as
much contamination from the old rinsing solution as possible.
8 If required, repeat steps 1-6 one or two times, to ensure that all
contamination is washed out.
Change direction
A B A&B
Pump P-920
User Manual 18-1125-54 Edition AB
17
Maintenance
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18-1125-54_ab.qxd 1998-10-22 12:49 Sida 17 (1,1)