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37 – LSM 880 Training Guide
Using AiryScan
FAST
The AiryScan FAST detection system is designed for imaging with improved resolution at speeds that are significantly faster than
traditional confocal – even with the base LSM 880.
1.
From the Acquisition toolbar, expand the blue
dialog labeled ‘Imaging Setup’.
2.
Select ‘FAST’ mode.
3.
Begin with the longest wavelength fluorophore
on Track 1.
Note: By default, ZEN will start with one track
(Track 1). To add additional tracks, click the +
button next to the track list.
4.
Click ‘Visible Light’ to open the laser dialog.
5.
Select the laser line you wish to use for this track.
6.
Set laser power slider (this is a starting value and
can be changed later).
Note: The laser power in the FAST mode is reported differently compared to the base LSM. The first value is the percentage
laser power for the FAST mode and is equivalent to the second value in the LSM mode. For example, 2% of 561nm in FAST
mode is equivalent to 0.25% in LSM mode.
7.
Set MBS (Main Beam Splitter) to a filter that matches the combination of laser lines you will need for ALL TRACKS.