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30 – LSM 880 Training Guide
Using AiryScan
8.
Select the appropriate emission filter for the fluorophore you wish to
image.
Note: The emission filters on the AiryScan are dual bandpass filters. These
are configured with common combinations to limit the filter wheel from
changing between tracks which can slow the system down. When
choosing this filter, try to choose the best filter that matches two of your
fluorophores. In our example, we would want to choose a filter for both
the Alexa 488 and the Alexa 568, even though the first track will only image
the Alexa 568.
9.
Click + button to add another track. Repeat steps 3-8 for the next
fluorophore.
Note: If you choose the ‘Line’ option for Switch track after, you cannot
change emission filters between tracks. You must choose ‘Frame’ for this
purpose.