11 – LSM 880 Training Guide
Light Path Configuration
In the following example, we will design a 2 fluorophore (Alexa 568 and Alexa 488) sequential acquisition using the line-wise track
switch.
1.
From the Acquisition toolbar, expand the blue dialog labeled
‘Imaging Setup’ and set ‘Switch track every’ to ‘Line’.
2.
Begin with the longest wavelength fluorophore (Alexa 568 in
this case) on Track 1.
Note: By default, ZEN will start with one track (Track 1). To add
additional tracks, click the + button next to the track list.
3.
Click ‘Visible Light’ to open the laser dialog.
4.
Select the laser line you wish to use for this track (561 for Alexa
568 in this case).
5.
Set laser power slider – in this example, we are using 2% (this is
a starting value and can be changed later).
6.
Set MBS (Main Beam Splitter) to a filter that matches the
combination of laser lines you will need for ALL TRACKS.