Zeiss LSM 710, Quick Manual

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Carl Zeiss
LSM710 / 780 ZEN
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9
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4-2. Smart Setup
① Click on the
Smart Setup
button to open the
smart setup window.
② Click on the arrow in the dye list and simply
choose the dye(s) you use in your experiment
from the list dialogue. In this dialogue, the
candidate dyes can be also searched by typing
the name in the search field.
③ Add or delete dye name by click on + or – .
④ Once finished with the input, Smart Setup suggests four alternative considerations
(see below): One for fastest imaging, one for the best signal , best compromise
between both speed and best signal and the optimal setup for later linear unmixing
of the dyes.
⑤ Click on the Apply , automatically sets the ideal hardware parameters for the dyes
chosen.
Fastest (Simultaneous)
− Advantage: faster image acquisition
− Disadvantage: potential cross-talk between channels
Best Signal (Sequential)
− Advantage: Only one detector and one laser are switched on at any one
time. This reduces cross-talk.
− Disadvantage: slower image acquisition
Best Compromise (Semi-Sequential)
− Best compromise between Fastest and Best signal.
②
③
④
Fig13. Smart setup tool
①
Fig.12 Smart setup