Carl Zeiss
LSM710
/
780 ZEN
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17
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Averaging
Averaging improves the image by increasing the signal-to-noise ratio. Averaging scans
can be carried out line-by-line or frame-by-frame. Frame averaging helps to reduce photo-
bleaching, but does not give quite as smooth of an image.
For averaging, select the number of lines or frames
to average.
Scan Area
In this panel, the scan field is set for zoom, rotation and offset in relation to the field of
view of the microscope. The diagonals of the outer square on the left-hand side
correspond to the field of view of the
microscope.
The inner square contained in it
(rectangle in the case of differently set
frame size) represents the scan range and
immediately shows the changes made to
zoom, rotation and offset.
By clicking on the Reset All
button the scan zoom is set to 1, the XY offsets are set to
the zero position and the ratio angle is set to 0°
⑦
Finally, click on Snap
for recording an image.
⑧
Save the image (Chapter 6), or if you scan next image, click on New
to open an
empty image window.
The next acquired image is displayed in this image window.
※
Remember, an unsaved 2D image in the active image tab will be over-written by a new scan. Multi-
dimensional scans or saved images will never be over-written and a new scan image will be displayed in a
new image window.
※
Acquired data is
not
automatically saved to disc. Make sure you save your data appropriately and back it up
regularly. The ZEN software will ask you if you want to save your unsaved images when you try to close the
application with unsaved images still open.
Fig.25 Averaging
Snap
Fig.26 Scan Area
Summary of Contents for LSM 710
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