Zeiss LSM 710, Quick Manual

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Carl Zeiss
LSM710 / 780 ZEN
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Select the Acquisition Mode tool from the Left Tool Area.
Frame Size
Select the Frame Size as predefined number of pixels or enter your own values (default is
512 x 512) in the Acquisition Mode tool. Click on the Optimal button for calculation of
appropriate number of pixels depending on objective N.A. and λ .
※ The number of pixels influences the image resolution!
Speed
Use the Scan Speed slider in the Acquisition Mode tool to adjust the scan speed.
※ A higher speed with averaging results in the best signal-to-noise ratio. Scan speed 8 usually
produces good results. Use speed 6 or 7 for superior images.
Bit Depth
Select the dynamic range 8, 12 or 16 Bit (per pixel) in the Bit Depth pull-down in the
Acquisition Mode tool
※ 8 Bit give 256 gray levels; 12 Bit give 4,096 gray levels, 16 Bit gives 65,536 gray levels.
Publication quality images should be acquired using 12 Bit data depth. 12 Bit is also
recommended when doing quantitative measurements or when imaging low fluorescence
intensities.
Setting the parameters for scanning
Fig.24 Acquisition Mode