Carl Zeiss
LSM710
/
780 ZEN
-
22
-
7. Z stack
The Z-Stack function permits scanning a series of XY-images in different focus positions
resulting in a Z-Stack, thus producing 3 dimensional data from your specimen.
①
Select
Z-Stack
in the main tools area.
Open the Z Stack tool in the Left Tool
Area.
②
Select
Mode First/Last
on the top of the
Z-Stack
tool.
③
For the definition of the first and last image
of the stack ;
1)
Click on the
button in the
Action
Button
area. A continuous XY-scan of the
set focus position will be performed.
2) Use the focus drive of the microscope to
focus on the upper position of the
specimen area where the Z Stack is to start. Click on the
Set First
button to set the upper
position of the Z Stack.
3) Then focus on the lower specimen area where the recording of the Z Stack is to end. Click
on the
Set Last
button to set this lower position.
4) Click on the
button to stop the scanning.
Fig.34 Application
Scanning a Z stack
Summary of Contents for LSM 710
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