Carl Zeiss
LSM710
/
780 ZEN
-
12
-
Fig.17 Channels tool
5. Scanning an image
①
Select the
Channels
tool in the Left Tool Area. The
Channels
tool provides the control of
the parameters for the individual detection channels.
②
Set the Pinhole size to
1 AU
(Airy unit) for best compromise between depth discrimination
and detection efficiency.
※
Pinhole adjustment changes the Optical Slice thickness. When collecting multi-channel images, adjust
the pinholes so that each channel has the same Optical Slice thickness. This is important especially for
colocalization studies.
Adjusting pinhole size
↓
Auto Exposure
↓
Live scan
Adjusting Brightness/Contrast
↓
Setting the parameters
↓
Snap
Adjusting pinhole size
Workflow to acquire images
Summary of Contents for LSM 710
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Page 32: ...Carl Zeiss LSM710 780 ZEN 30 10 Operation of Light Microscope Axio Observer Z1...
