Zeiss LSM 710, Quick Manual

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Carl Zeiss
LSM710 / 780 ZEN
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15
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Fig.23 Range Indicator
Choosing Range Indicator
In the View – Dimensions View Option Control Block, click inside the color field in the
button under the channel button (Fig. 22)
Image Optimization
The scanned image appears in a false-color
presentation
If the image is too bright, it appears red on the
screen. Red = saturation (maximum).
If the image is not bright enough, it appears
blue on the screen. Blue = zero (minimum).
Adjusting the laser intensity
• Set the Pinhole to 1 Airy Unit (Fig. 25)
• Set the Gain (Master) high.
• When the image is saturated, reduce AOTF transmission in the Laser control section of the
Channels Tool (Fig. 25) using the slider to reduce the intensity of the laser light to the
specimen.
Adjusting gain and offset
• Increase the Digital Offset until all blue pixels disappear, and then make it slightly positive.
• Reduce the Gain (Master) until the red pixels only just disappear.
Fig.21 Dimensions Control window