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20
01/2013
Setting scan averaging
Averaging improves the image by increasing the signal-to-noise ratio. Averaging scans can be carried out
line-by-line or frame-by-frame. Frame averaging helps to reduce photo-bleaching, but does not give quite
as smooth of an image.
x
For averaging, select the
Line
or
Frame
mode in the
Acquisition Mode
tool.
x
Select the number of lines or frames to average.
Adjusting pinhole
x
Select the
Channels
tool in the
Left Tool Area
.
x
Set the
Pinhole
size to
1 AU
(Airy unit) for best compromise between depth discrimination and
detection efficiency.
Pinhole adjustment changes the
Optical Slice thickness
. When collecting multi-channel images, adjust
the pinholes so, that each channel has the same
Optical Slice thickness
. This is important for
colocalization studies.
o
o
Fig. 21
Channels tool
Summary of Contents for LSM 700
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