Use of the Axioskop (Zeiss)
The Axioskop is equipped for viewing samples with
transmitted light (from a halogen lamp
source)
and has optics for:
Bright field, Phase Contrast and Differential Interference Contrast
(DIC)
microscopy. Samples can also be viewed for
fluorescence microscopy
using the incident-
light system (front a mercury lamp source).
1)
For transmitted light microscopy work
, press the
green on/off light switch (1a) at the back-right side of the
microscope while light intensity / level knob is located
next to switch (1b). The halogen lamp is in the vented
box at the bottom rear of the scope (1c).
2)
For fluorescence microscopy work
, press green on/off light switch (2a) on the front of the
arc lamp power supply box behind the scope (right side- 2b). The mercury lamp is in the vented
housing on the rear of the scope and is above the halogen lamp box (2c).
*Note: the halogen lamp does not need to be on when only viewing fluorescence
Transmitted light microscopy (Brightfield/DIC)
Begin with glass slide/specimen securely in place on stage, light at medium
brightness/temperature (~6).
Ocular's (eyepieces) diopter rings set
at "white dot-to-0-position".
In most
cases start with 10 x objective lens in
2a
2b
2b
2c
1a
1b
1c
