position
. Obtain a "basic" focus using the coarse (large inner black knob), and then if desired,
the fine (smaller outer knob) coaxial focusing control knobs (on the back left and right side of
the microscope base).
*Note: one revolution of the course knob = ~2 mm of stage travel toward any Z-direction
Kohler Alignment
To achieve maximum even illumination of the sample,
the condenser needs to be properly
focused and adjusted
(Kohler Alignment)
1)
Put the specimen in focus in the light path, then pull out the
Analyzer
[thin black plastic
slider with dark filter that is below and to right of the ocular and the large black filter slider].
Turn/rotate
counter-clockwise
the
lower
Polarizer
using its
black lever (at the
bottom of the
condenser and above the field diaphragm) so they are not in the
light path.
*Note: for
Brightfield
/
DIC adjustment
, the black horizontal metal filter slider should be in the
"no-filter/ open position"
(last position on left or 5th slot to left; pulled to the extreme right).
**Note: After adjustment, f
or Brightfield use
, you can leave analyzer & bottom polarizer in
OUT position.
For DIC use
, analyzer and polarizer can be returned (slide IN) to the light path.
2)
Transmitted light can be controlled by adjusting the
black circular
field diaphragm
lens
(3a)
at the base of the microscope by turning its outer ring clockwise (opens) or
counterclockwise (closes).
To adjust the condenser and focus the light
, put the sample on the
stage look into the ocular and focus it.
Then close the field diaphragm,
center the "small" light spot by
turning
the left and right silver
color centering screws (3b
- Two
silver screws at 7 & 5 o'clock on
the front of the condenser).
Pull out
Analyzer
3a
3b
3b
3c
3d