Zeiss Axioskop User Manual Download Page 7

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Zeiss Axioskop Fluorescence Reflector Slider

The first (right-most) position: (for FITC/ AF488/ GFP - type fluorophores

)

Excitation filter: 450 - 490 nm
Dichroic mirror: 495 nm
Emission filter: 500 - 550 nm

Second position: (for Rhodamine/ AF594/ Texas red - type fluorophores)

Excitation filter: 510- 560 nm
Dichroic mirror: 580 nm
Emission filter: 590 nm LP

Third position: (for YFP - type fluorophores)

Excitation filter: 450 - 490 nm
Dichroic mirror: 510 nm
Emission filter: 520 nm LP

Fourth position: (for DAPI / Hoeschst - type fluorophores)

Excitation filter: 300(365)390 nm
Dichroic mirror: 395 nm
Emission filter: 420 nm LP

Fifth position: (for DIC / Brightfield)

OPEN (no filters)

Summary of Contents for Axioskop

Page 1: ...ZEISS AXIOSKOP Microscope s User Manual Managed by For information about this instrument please contact Dr Alloysius Budi Utama ext 8232 or e mail budiutama rice edu...

Page 2: ...ed next to switch 1b The halogen lamp is in the vented box at the bottom rear of the scope 1c 2 For fluorescence microscopy work press green on off light switch 2a on the front of the arc lamp power s...

Page 3: ...he field diaphragm so they are not in the light path Note for Brightfield DIC adjustment the black horizontal metal filter slider should be in the no filter open position last position on left or 5th...

Page 4: ...different objective lenses magnifications For example if condenser is focused for 20x that could be acceptable for 10x and 40x air objectives focus for 63x could be acceptable for 100x oil objectives...

Page 5: ...en light color temperature of 3200 K into natural outdoor light color temperature of 5500 K When push buttons are IN or OUT the corresponding filters are IN or OUT as well Fluorescence microscopy Afte...

Page 6: ...orescence that contain various excitation filter dichroic mirror emission filter sets from right to left 450 490 um ex green em 510 560 nm ex red em 450 490 nm ex green yellow em 300 390 nm ex blue em...

Page 7: ...ne AF594 Texas red type fluorophores Excitation filter 510 560 nm Dichroic mirror 580 nm Emission filter 590 nm LP Third position for YFP type fluorophores Excitation filter 450 490 nm Dichroic mirror...

Page 8: ...contrast condenser position DIC Plan NEOFLUAR 40X NA 0 75 Ph 2 air n 1 Phase contrast condenser position 2 Brightfield condenser position HDIC DIC contrast condenser position DIC Plan NEOFLUAR 63X NA...