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 START-UP 

Axioskop 

2 

plus

 

Carl Zeiss 

List of illustrations 

Axioskop 2 

mot plus

 

 

 
 
2-2 

B 40-075 e    10/03 

List of illustrations  

Fig. 2-1

 

Setting up the microscope ................................................................................................................ 2-3

 

Fig. 2-2

 

Attachment of binocular tube........................................................................................................... 2-4

 

Fig. 2-3

 

Inserting the eyepieces...................................................................................................................... 2-4

 

Fig. 2-4

 

Inserting the eyepiece reticle............................................................................................................. 2-5

 

Fig. 2-5

 

Setting the interpupillary distance of the binocular tube ................................................................... 2-6

 

Fig. 2-6

 

Screwing in of objectives .................................................................................................................. 2-6

 

Fig. 2-7

 

Attachment of universal condenser................................................................................................... 2-7

 

Fig. 2-8

 

Attachment of universal condenser MOT .......................................................................................... 2-7

 

Fig. 2-9

 

Axioskop 2 

plus

 (rear) ....................................................................................................................... 2-8

 

Fig. 2-10

 

HBO 100 W transformer (front and rear) .......................................................................................... 2-8

 

Fig. 2-11

 

Attachment of HAL 100 halogen illuminator .................................................................................... 2-9

 

Fig. 2-12

 

Adjustment of HAL 100 halogen illuminator ..................................................................................... 2-9

 

Fig. 2-13

 

Attachment of HBO 103 illuminator ............................................................................................... 2-10

 

Fig. 2-14

 

HBO 100 W transformer ................................................................................................................. 2-11

 

Fig. 2-15

 

Adjusting aid .................................................................................................................................. 2-11

 

Fig. 2-16

 

Adjusting HBO 103......................................................................................................................... 2-11

 

Fig. 2-17

 

Focal spots of HBO 103 before coarse adjustment .......................................................................... 2-12

 

Fig. 2-18

 

Focal spots of HBO 103 after coarse adjustment............................................................................. 2-12

 

Fig. 2-19

 

Focal spot imaging via adjusting aid and knurled knob for focusing ............................................... 2-12

 

Fig. 2-20

 

Focal spot imaging without adjusting aid on the mechanical stage ................................................. 2-13

 

Fig. 2-21

 

Axioskop 2 

mot plus

 (rear) .............................................................................................................. 2-14

 

Fig. 2-22

 

Changing the condenser ................................................................................................................ 2-15

 

Fig. 2-23

 

Attachment of condenser MOT ...................................................................................................... 2-16

 

Fig. 2-24

 

Changing the phase stop................................................................................................................ 2-16

 

Fig. 2-25

 

Changing the DIC prism ................................................................................................................. 2-17

 

Fig. 2-26

 

Attachment of filter mount............................................................................................................. 2-17

 

Fig. 2-27

 

Attachment of D polarizer .............................................................................................................. 2-18

 

Fig. 2-28

 

Attachment of SENARMONT DIC polarizer ..................................................................................... 2-18

 

Fig. 2-29

 

Changing the halogen lamp ........................................................................................................... 2-19

 

Fig. 2-30

 

HBO 100 W transformer ................................................................................................................. 2-20

 

Fig. 2-31

 

Removing the HBO 103 illuminator................................................................................................. 2-20

 

Fig. 2-32

 

Removing the housing of the  HBO 103.......................................................................................... 2-21

 

Fig. 2-33

 

Changing the HBO 103 W/2 mercury pressure short-arc lamp ........................................................ 2-21

 

Fig. 2-34

 

Changing the fixed mechanical stage ............................................................................................. 2-22

 

Fig. 2-35

 

Changing the rotary mechanical stage............................................................................................ 2-22

 

Fig. 2-36

 

Centering the rotary mechanical stage ........................................................................................... 2-23

 

Fig. 2-37

 

Setting ergo-drive ........................................................................................................................... 2-24

 

Fig. 2-38

 

Changing the specimen holder ....................................................................................................... 2-25

 

Fig. 2-39

 

Changing the binocular tube .......................................................................................................... 2-25

 

Fig. 2-40

 

Attachment of intermediate tube with height adjustment .............................................................. 2-26

 

Fig. 2-41

 

Attachment of adapter for the Axioskop 2 

plus

.............................................................................. 2-27

 

Fig. 2-42

 

Changing the technique module .................................................................................................... 2-28

 

Fig. 2-43

 

Changing the filter set in the P&C FL reflector module ................................................................... 2-29

 

Fig. 2-44

 

Changing the beam splitter ............................................................................................................ 2-29

 

Fig. 2-45

 

Changing the beam splitter ............................................................................................................ 2-30

 

Fig. 2-46

 

Attachment of active coobservation equipment for 2 users ............................................................ 2-32

 

Fig. 2-47

 

Attachment of multi-observation equipment .................................................................................. 2-34

 

Fig. 2-48

 

Attachment of intermediate tube for image projection................................................................... 2-35

 

Summary of Contents for Axioskop 2 plus

Page 1: ...Operating Manual Axioskop 2 plus Axioskop 2 mot plus Routine Microscope ...

Page 2: ...not covered by an update service Unless expressly authorized forwarding and duplication of this document and the software package and the utilization and communication of its contents are not permitted Violations will entail an obligation to pay compensation All rights reserved in the event of granting of patents or registration of a utility model Issued by Carl Zeiss Light Microscopy P O B 4041 3...

Page 3: ... view of Axioskop 2 plus 0 13 Overall view of Axioskop 2 mot plus 0 14 CHAPTER 1 INSTRUMENT DESCRIPTION 1 2 1 1 Name and intended application 1 2 1 2 Instrument description and main features 1 2 1 3 Axioskop 2 plus manual microscope configurations and modules 1 4 1 4 Axioskop 2 mot plus microscope configurations and modules 1 8 1 5 Objectives 1 12 1 6 Eyepieces 1 14 1 7 Stage micrometers and eyepi...

Page 4: ... 2 2 1 Changing the condenser 2 16 2 2 2 Changing the phase stop for phase contrast or darkfield in the universal condenser 2 17 2 2 3 Changing the DIC prism in the universal condenser 2 18 2 2 4 Attachment of filter mount 2 18 2 2 5 Attachment of D polarizer 2 19 2 2 6 Attachment of SENARMONT DIC polarizer 2 19 2 2 7 Changing the HAL 100 halogen lamp 2 20 2 2 8 Changing the HBO 103 W 2 mercury pr...

Page 5: ...ght darkfield 3 27 3 3 3 Setting of transmitted light phase contrast 3 29 3 3 4 Setting of transmitted light polarization 3 31 3 3 5 Setting of transmitted light differential interference contrast DIC 3 36 3 3 6 Setting of epi fluorescence 3 39 3 4 Documentation 3 41 3 4 1 Attachment of photomicrography equipment 3 41 3 4 2 Attachment of videomicroscopy equipment 3 43 3 4 3 Attachment of adapter f...

Page 6: ...TENANCE TROUBLESHOOTING AND SERVICE 4 2 4 1 Instrument care 4 2 4 2 Instrument maintenance 4 3 4 2 1 Performing checks 4 3 4 2 2 Changing the fuses 4 3 4 3 Troubleshooting 4 4 4 4 Spares consumables and tools 4 8 4 5 Requesting service 4 9 ANNEX A 1 List of abbreviations A 2 List of key words A 4 Patent rights A 8 ...

Page 7: ...uch a way that the specimen is not touched by the condenser 5 Close luminous field diaphragm until it is visible in the field of view even if not in focus Fig 0 1 A 6 Lower universal condenser 0 9 until the edge of the luminous field diaphragm appears in focus Fig 0 1 B 7 Center luminous field diaphragm using centering screws on the condenser carrier Fig 0 1 C and open it until its edge just disap...

Page 8: ...ts of the directive 98 79 EC and are marked with the marking This operation manual includes information and warnings which must be observed by the user The following warning and information symbols are used in this manual NOTE This symbol is a warning which you must observe under all circumstances CAUTION This symbol is a warning which indicates a hazard to the instrument or instrument system CAUT...

Page 9: ...nadvertent operation Please contact a Zeiss service agency or Carl Zeiss Microscopy Service for repairing the instrument The power unit which is integrated in the stand of the microscopes permits the use of line voltages in the range between 100 and 240 V 10 50 60 Hz without having to change the voltage on the instrument The ballasts for the HBO 103 ebg 100 dc or XBO 75 ebx 75 isolated are designe...

Page 10: ... g special attenuation filters When hot gas discharge lamps are under high internal pressure and may therefore only be changed when they have cooled down and protective gloves and goggles must be used for detailed information please see Operating Manual B 40 065 e When fluorescence filters are used the filter protecting from heat emitted by the microscope illuminator must not be removed since fluo...

Page 11: ...ed in its performance or destroyed when handled improperly Be sure to read the safety notes provided with Immersol 518 N immersion oil Immersol 518 N immersion oil irritates the skin Avoid any contact with skin eyes and clothing After skin contact wash the oil off with plenty of water and soap After eye contact immediately rinse the eye with plenty of water for at least five minutes If the irritat...

Page 12: ...rantee is provided for defects caused by natural wear wearing parts in particular and improper use The instrument manufacturer is not liable for damage caused by faulty operation negligence or any other meddling with the instrument particularly the removal or replacement of instrument components or the use of accessories from other manufacturers This forfeits all the claims against warranty With t...

Page 13: ...op 2 plus Carl Zeiss B 40 075 e 10 03 0 13 Overall view of the Axioskop 2 plus 1 Eyepieces 2 Binocular tube 3 Stand 4 Incident light illuminator 5 Transmitted light illuminator 6 Manual universal condenser 7 Mechanical stage 8 Objectives 1 0 2 0 3 0 4 0 5 0 6 0 7 0 8 0 90 10 0 ...

Page 14: ...us Axioskop 2 mot plus 0 14 B 40 075 e 10 03 Overall view of the Axioskop 2 mot plus 1 Eyepieces 2 Binocular ergotube 3 Stand 4 Incident light illuminator 5 FL excitation filter wheel 6 Transmitted light illuminator 7 Motorised universal condenser 8 Mechanical stage 9 Objectives ...

Page 15: ...cription and main features 1 2 1 3 Axioskop 2 plus manual microscope configurations and modules 1 4 1 4 Axioskop 2 mot plus microscope configurations and modules 1 8 1 5 Objectives 1 12 1 6 Eyepieces 1 14 1 7 Stage micrometers and eyepiece reticles 1 14 1 8 Technical Data 1 16 List of illustrations Fig 1 1 Axioskop 2 plus manual microscope configurations 1 4 Fig 1 2 Axioskop 2 mot plus microscope ...

Page 16: ...ern demands on design operating convenience and function with technical performance Thanks to the modular design existing microscope modules of the Axioskop 2 plus such as tubes intermediate tubes and condensers are easy to attach The Axioskop 2 plus is offered as a manual and a motorized coded version The basic stand is equipped with either manual Axioskop 2 plus or motorized coded operation and ...

Page 17: ...an Plan Neofluar Plan Apochromat Binocular tubes and phototubes with fixed and variable viewing angle 10x focusing eyepieces with field number 23 suitable for spectacle wearers High performance epi fluorescence equipment Wide variety of attachment possibilities for photography and videomicroscopy Multi observation equipment for 2 9 or more viewing stations Drawing device with intermediate tube for...

Page 18: ...xioskop 2 plus microscope configurations Axioskop 2 mot plus 1 4 B 40 075 e 10 03 1 3 Axioskop 2 plus manual microscope configurations and modules Fig 1 1 Axioskop 2 plus manual microscope configurations 1 1 1 1 1 1 0 2 0 3 0 4 0 5 0 6 0 7 0 8 0 90 10 0 ...

Page 19: ...Eyepiece 3 Binocular tube 30 23 4 HBO 100 W transformer 5 HBO 103 illuminator 6 HAL 12 V 100 W halogen lamp 7 FL filter slider 8 Objective 9 DIC slider 10 Compensator λ 11 Dust cover 12 DIC prism 13 Achromatic aplanatic universal condenser 0 9 H D DIC 14 Filter mount 15 Mechanical stage 75x50 R 16 Specimen holder with spring clip R 17 FL P C reflector module ...

Page 20: ...40x Plan Apochromat 63x and Plan Neofluar 100x Modules The Axioskop 2 plus routine microscope consists of the following modules Axioskop 2 plus microscope stand with 5x reflector turret harmonic drive focusing drive 5 position nosepiece H DIC W 0 8 integrated 12 V DC 100 W power unit stabilized transmitted light equipment with filter magazine D stage and condenser carrier FL incident light equipme...

Page 21: ...ating and fixed coaxial drive 130 mm 453505 9901 000 453502 9905 000 453523 0000 000 Specimen holder with spring clip R 453533 0000 000 Technique modules FL P C reflector module e g with filter sets for epi fluorescence filter set 01 UV excitation H 365 filter set 09 blue excitation 450 490 filter set 15 green excitation H 546 DIC FL analyzer Analyzer module D Optovar module 1 25x Optovar module 1...

Page 22: ...ioskop 2 plus Carl Zeiss Axioskop 2 mot plus microscope configurations Axioskop 2 mot plus 1 8 B 40 075 e 10 03 1 4 Axioskop 2 mot plus microscope configurations and modules Fig 1 2 Axioskop 2 mot plus microscope configurations ...

Page 23: ... 6 25 23 4 HBO 100 W transformer 5 HBO 103 illuminator 6 HAL 12 V 100 W halogen illuminator 7 FL filter slider 8 Objective 9 DIC slider 10 Compensator λ 11 Dust cover 12 DIC prism 13 Achromatic aplanatic universal condenser 0 9 H D DIC MOT 14 Filter mount 15 Mechanical stage 75x50 240 R 16 Specimen holder with spring clip R 17 FL P C reflector module 18 8 position FL excitation filter wheel ...

Page 24: ...C 100 W power unit MOT stabilized transmitted light equipment stage and condenser carrier FL incident light and HBO XBO adjusting aid 452311 0000 000 Eyepieces W PL 10x 23 Br foc PL 10x 23 Br foc 000000 1016 758 000000 1026 548 Tubes Binocular tube 30 23 Binocular phototube 30 23 30 70 Binocular ergotube 6 25 23 Binocular ergo phototube 6 25 23 100 0 or 0 100 452340 0000 000 452344 0000 000 452341...

Page 25: ...for epi fluorescence filter set 01 UV excitation H 365 filter set 09 blue excitation 450 490 filter set 15 green excitation H 546 DIC FL analyzer Analyzer module D Optovar module 1 25x Optovar module 1 6x Optovar module 2 5x 000000 1046 281 488001 0000 000 488009 0000 000 488015 0000 000 452374 0000 000 000000 1050 958 000000 1046 284 000000 1046 283 000000 1046 282 Dust cover M medium Dust cover ...

Page 26: ...hite Magnification factor 1 25x 2 5x 4x 5x 6 3x 10x 16x 20x 25x 32x 40x 50x 63x 100x 150x The objective magnification multiplied with the eyepiece magnification minimum 10x results in the visual overall magnification e g 10 x 10 100x When working with the microscopes the total magnification should not drop below or exceed the range of useful magnification The range of useful magnification was defi...

Page 27: ...an Transmitted light brightfield Achroplan 4x 0 10 11 1 440020 0000 000 Achroplan 10x 0 25 4 8 440030 0000 000 Achroplan 20x 0 45 2 07 0 17 440040 0000 000 Achroplan 40x 0 65 0 59 0 17 440050 0000 000 Achroplan 50x 0 90 Oil 0 29 0 17 440057 0000 000 Achroplan 63x 0 80 0 29 0 17 440060 0000 000 Achroplan 63x 0 95 0 15 0 no cover slip 440068 0000 000 Achroplan 100x 1 25 Oil 0 19 0 17 440080 0000 000...

Page 28: ...nt image flatness up to the edge of the field of view PL 10x 23 Br foc eyepieces ensure excellent optical correction particularly in combination with the ergotubes If required eyecups for the eyepieces can be ordered under Cat No 444801 1 7 Stage micrometers and eyepiece reticles Measuring and counting using the microscope requires stage micrometers and eyepiece reticles a selection of which is li...

Page 29: ...ance 0 001 mm 474066 9901 000 Net micrometer 12 5x12 5 5 10 d 26 mm area 12 5x12 5 mm divided in fields of 10x10 474068 0000 000 Photo reticle MC 2 5x d 26 mm for 35 mm photography with an additional magnification of 2 5x or for large format photography with a 10x additional magnification 454075 0000 000 If an eyepiece reticle is used the binocular tube or the phototube must be equipped with two f...

Page 30: ...ox 18 4 kg Axioskop 2 mot plus approx 22 4 kg Ambient conditions Transport in packaging Permissible ambient temperature 40 to 70 C Storage Permissible ambient temperature 10 to 40 C Permissible humidity without condensation max 75 at 35 C Operation Permissible ambient temperature 5 to 40 C Permissible relative humidity max 75 at 35 C Altitude max 2000 m Atmospheric pressure 800 hPa to 1060 hPa Pol...

Page 31: ...A HBO 50 transformer mbq52ac z Operating environment clean room Protection class I Protection type IP 20 Line voltage can be changed between 100 110 120 127 VAC and 230 240 VAC Line frequency can be changed between 50 and 60 Hz Power consumption when HBO 50 is used max 350 VA Fuses in accordance with IEC 127 Axioskop 2 plus microscope stand T 4 A H 5x20 mm Axioskop 2 mot plus microscope stand T 4 ...

Page 32: ...r 7 positions for brightfield darkfield for phase contrast 1 2 3 for DIC I II III changeable Objective change manually via 6 position nosepiece H DIC W 0 8 Change of technique modules manually via 5 position reflector turret Axioskop 2 mot plus Stand with motorized stage focusing average step width of the step motor 80 nm fast stage lowering lifting in the range of 6 mm lifting range 25 mm height ...

Page 33: ... Changing the condenser 2 15 2 2 2 Changing the phase stop for phase contrast or darkfield in the universal condenser if required 2 16 2 2 3 Changing the DIC prism in the universal condenser 2 17 2 2 4 Attachment of filter mount 2 17 2 2 5 Attachment of D polarizer 2 18 2 2 6 Attachment of SENARMONT DIC polarizer 2 18 2 2 7 Changing the HAL 100 halogen lamp 2 19 2 2 8 Changing the HBO 103 W 2 merc...

Page 34: ...s rear 2 14 Fig 2 22 Changing the condenser 2 15 Fig 2 23 Attachment of condenser MOT 2 16 Fig 2 24 Changing the phase stop 2 16 Fig 2 25 Changing the DIC prism 2 17 Fig 2 26 Attachment of filter mount 2 17 Fig 2 27 Attachment of D polarizer 2 18 Fig 2 28 Attachment of SENARMONT DIC polarizer 2 18 Fig 2 29 Changing the halogen lamp 2 19 Fig 2 30 HBO 100 W transformer 2 20 Fig 2 31 Removing the HBO...

Page 35: ...r tube objectives eyepieces condenser 12 V 100 W illuminator microscope mat and various small parts e g DIC slider spare lamp dust cover tools bag The following components are factory attached to the microscope stand mechanical stage specimen holder filter mount technique modules and epi fluorescence equipment with adjusting aid Further optional accessories are supplied in a separate case Remove a...

Page 36: ...2 4 Turn the binocular tube in the required observation position and tighten the hexagonal screw using the ball headed screwdriver 2 1 3 Insertion of eyepieces and centering telescope Remove both dust covers 2 3 1 and 4 from the binocular tube Remove both eyepieces 2 3 2 from the cases and insert them in the binocular tube until stop The centering telescope 2 3 3 which is used to view the aperture...

Page 37: ...d eyepiece reticle If eyepiece reticles are inserted into the unscrewed mount by the customer attention must be paid to the labelling being visible the right way up after insertion Compensation of ametropia when eyepiece reticles are used The correct use of an eyepiece reticle requires two focusing eyepieces e g E PL 10x 23 Br foc to allow the user to compensate for differences in the visual perfo...

Page 38: ...rgotubes Furthermore the binocular component of the ergotubes can be swivelled by 180 2 1 6 Screw in of objectives Move mechanical stage with carrier to the lower stop position Remove dust caps 2 6 2 from the respective openings in the nosepiece Remove objectives 2 6 3 from case and screw them in nosepiece 2 6 1 clockwise one by one starting with the lowest magnification In the case of the Axiosko...

Page 39: ...ier 2 7 2 to the lowest position via drive for height adjustment 2 7 5 Insert condenser 2 7 1 between condenser carrier 2 7 2 and stage carrier 2 7 4 Orient the locking screw on the underside of the condenser in the direction of the groove 2 7 7 Press dovetail of the condenser against the spring mount 2 7 6 of the condenser carrier until the condenser can be placed horizontally on the condenser ca...

Page 40: ... automatically to the available line voltage within this range The HBO 103 illuminator for epi fluorescence is supplied via a separate transformer The HBO 100 W transformer must be connected to the line via its line socket 2 10 2 2 1 9 Switch microscope and HBO 100 W transformer on and off Switch the microscope on and off using the line switch 2 9 1 Switch the HBO 100 W transformer on and off usin...

Page 41: ... halogen illuminator a Coarse adjustment Loosen clamping screw 2 11 2 and remove the halogen illuminator from the microscope stand Switch on microscope as described under 0 Direct the light beam against a projection area wall approx 3 m away Do not look into the light exit opening of the illuminator Set adjusting screw 2 12 3 using SW 3 ball headed screwdriver in such a way that both images of the...

Page 42: ...inator 1 Attach HBO 103 W 2 mercury vapor short arc lamp The HBO 103 illuminator and the HBO 103 W 2 mercury vapor short arc lamp are supplied in separate packages for safety reasons Therefore insertion of the HBO 103 W 2 lamp into the lamp housing is the first step to the start up of this illuminator Insertion of a new HBO 103 W 2 lamp is described in detail on page 2 19 section 2 2 8 entitled Ch...

Page 43: ...Coarse adjustment Remove HBO 103 illuminator from the microscope stand by loosening clamping screw 2 13 2 Switch on HBO 103 on HBO 100 W transformer 2 10 1 and allow it to heat to operating temperature Make sure not to look into the light exit opening of the illuminator Direct light beam against a projection area wall approx 3 m away Used knurled knob 2 16 6 to focus on the brighter focal spot via...

Page 44: ...nd as described in section 2 1 11 2 Pull adjusting aid 2 15 2 out of the incident light tube 2 15 1 The brighter focal spot of the HBO 103 W 2 lamp and its slightly darker reflector image become visible in the black glass window of the adjusting aid Use knurled knob 2 16 6 or 2 19 to focus on the brighter focal spot via collector adjustment Apply SW 3 ball headed screwdriver on adjusting screw 2 1...

Page 45: ...e stand as described in section 2 1 11 2 Remove one objective from the nosepiece and switch empty opening in function position Place a white sheet of paper on the mechanical stage Fully open the precentered luminous field and aperture diaphragms in the incident light illumination beam path The pupil image with the two focal spot images is now visible on the white sheet of paper If required reduce ...

Page 46: ...2 21 3 and combined CAN SUB BUS 2 21 1 connectors 2 1 13 Attachment of round cable holder The round cable holder supplied with the mechanical stages is suitable to hold and attach electric cables e g cables of dictating machines The round cable holder should be adhered to a suitable place on the microscope stand The round cable holder is also suitable for holding and storing the size 2 screwdriver...

Page 47: ...he left of the stand of connecting cable and pull off the connecting cable Unscrew both centering screws 2 22 3 until condenser can be easily taken out of its mount Lift available condenser from the orientation groove by slightly tilting it upwards and pull it out to the front Use lever 2 22 8 to fold out the front lens on the condenser to be inserted or in the case of the motorized version swing ...

Page 48: ...loosen the cover on the condenser underside 2 24 2 loosen both grub screws and remove the cover Position turret disk with phase stop to be changed in the exchange opening and hold it on the knurled ring since no click stop is effective in this position Use mounting device contained in tool set to unscrew retainer ring 2 24 4 from the condenser underside 2 24 2 and allow phase stop 2 24 3 to slide ...

Page 49: ...ove DIC prism 2 25 2 from the tool and screw on the new required DIC prism Installation of the DIC prism is made in reverse order Make sure that the DIC prism is oriented correctly groove 2 25 5 must engage in the pinion of the mount Make sure that the knurled ring of the turret disk is labelled correctly 2 2 4 Attachment of filter mount Hold filter mount 2 26 5 parallel to the underside of the co...

Page 50: ...top bolt 2 27 2 with the adjusting lever 2 27 1 as far as the stop in the rear threaded hole of the condenser carrier 2 27 3 2 2 6 Attachment of SENARMONT DIC polarizer Hold polarizer 2 28 6 parallel to the underside of the condenser carrier 2 28 3 and screw stop bolt 2 28 4 of the polarizer 2 28 6 into the threaded hole on the left below the condenser carrier 2 28 3 until stop using the angled ad...

Page 51: ...et go the spring lever and pull the protection cap off Press spring lever briefly downwards again to center the lamp Attach lamp housing again and tighten clamping screw 2 29 5 2 2 8 Changing the HBO 103 W 2 mercury pressure short arc lamp Before opening the lamp housing make sure that no connection to the electrical line is available The HBO 203 W 2 lamps may only be changed after they have coole...

Page 52: ...n clamping screw 2 31 2 and remove HBO 103 illuminator 2 31 3 from the incident light tube 2 31 1 of the microscope stand Use focusing knob 2 32 3 to bring collector to the position at the very front in the direction of the light Use ball headed screwdriver to loosen clamping screw for lamp housing 2 32 2 and carefully remove lamp housing 2 32 1 from the lamp mount 2 33 3 in an upward direction Pr...

Page 53: ...t apply force to the lamp The holder in the heat sink is marked with H for HBO or X for XBO Clamp the fixing screw on the heat sink Insert the new lamp 2 33 1 with heat sink with the spring clip 2 33 4 pressed down in the holder provided in the illuminator socket 2 33 2 Make sure that the power cable to the heat sink is free at the side Release the spring clip Do not apply force to the lamp Fit th...

Page 54: ...rrier 2 34 6 in such a way that the threaded holes 2 34 2 on the underside of the stage are positioned above the openings of the stage carrier 2 34 3 Insert four fixation screws 2 34 5 through the stage carrier from below and screw them in the stage underside use the shorter screws on the front Orient stage in x y direction and tighten the fixation screws 2 Rotary mechanical stage 75x50 240 R a Re...

Page 55: ...ecentering should be performed as follows Loosen stage clamping screw 2 36 4 and screwing cap of the stage carrier 2 36 1 Rotate the stage to determine the maximum specimen deflection 2 36 5 tip of the arrow in the direction of the eyepiece reticle Reset the two centering screws on the stage carrier 2 36 2 using one SW 1 5 Allen screwdriver 2 36 3 each to move the specimen detail by half the arrow...

Page 56: ...he additional sleeves back on the drive knobs in reverse order and tighten both the clamping screws 5 Setting the smoothness torque of the two drive knobs of the ergo drive The ergo drive is set to a medium smoothness value at the factory This setting can be changed as follows a Adjusting the X direction Remove the additional sleeves 2 37 1 and 3 from the drive knobs if necessary Loosen the clampi...

Page 57: ...2 38 2 from the mechanical stage 2 38 4 in an upward direction Attach required specimen holder 2 38 5 to the mechanical stage and tighten two countersunk screws 2 2 10 Changing the binocular tube Use SW 3 ball headed screwdriver to loosen clamping screw 2 39 3 and remove available tube in an upward direction Place dust cap 2 39 2 for tube lens protection above the dovetail of the binocular tube Re...

Page 58: ...dovetail of the intermediate tube with height adjustment 2 40 5 Insert dovetail of intermediate tube in the stand opening 2 40 8 and tighten clamping screw 2 40 7 only slightly Align intermediate tube with the outer edges of the stand then tighten clamping screw 2 40 7 Unscrew tube lens 2 40 2 from the binocular ergotube 2 40 1 The tool from the storage case 2 40 3 must be used to remove the tube ...

Page 59: ...an upward direction Insert dovetail of the adapter for the Axioskop 2 plus 2 41 3 in the stand opening 2 41 6 on the underside and slightly tighten clamping screw 2 41 5 Insert fixation screw 2 41 2 in countersunk hole of the adapter 2 41 3 and screw it into the drilled hole 2 41 4 on the stand Tighten clamping screw 2 41 5 and fixation screw 2 41 2 Attach required intermediate tube and or tube 2 ...

Page 60: ...ret Then press the module at the top until it also snaps securely into the top spring clamps of the reflector turret Aligning the respective module when installing it in the reflector turret FL P C reflector module the excitation filter is facing forwards the emission filter is facing upwards Optovar module the lens is facing upwards the empty module opening is facing forwards Analyzer module the ...

Page 61: ...ce The barrier filter is inserted at 2 43 2 the excitation filter at 2 43 4 and both are secured using retainer ring 2 43 1 2 2 15 Changing the beam splitter in the FL P C reflector module Attachment of filters and of the beam splitter requires utmost care to prevent damage and contamination of the optical components We would recommend you to order completely equipped FL P C reflector modules sinc...

Page 62: ...m module half Make sure that the side latch of the spring frame is in the appropriate recess in the bottom module half The reflective side of the beam splitter is recognizable by carefully placing a wooden splint on the surface of the beam splitter There is no spacing between the splint and its mirror image Place the top module half 2 45 1 on the bottom module half 2 45 4 the retaining elements 2 ...

Page 63: ...clamping screw 2 46 12 to prevent the coobservation equipment from shifting Unscrew the tube lenses from the two binocular tubes using the cover of one of the storage cases and put them in the cases Remove upper dust caps 2 46 3 and 18 from the coobservation equipment The front dovetail 2 46 17 of the coobservation equipment allows attachment of either the binocular ergotube 6 25 23 2 46 1 or the ...

Page 64: ... equipment for 2 users 1 Binocular ergotube 2 Binocular tube 3 Dust cap 4 Rear dovetail 5 Socket for power supply 6 Heat protection shield 7 Fixation screw 8 Clamping screw 9 Support point stand 10 Power unit 11 Plug 12 Clamping screw 13 Dovetail stand 14 Dust cap 15 Dovetail 16 Coobservation equipment 17 Front dovetail 18 Dust cap 19 Clamping screw ...

Page 65: ...ten the captive coupling nuts Attach and clamp two binocular tubes for the multi observation equipment 2 47 6 on each of the connecting pieces L 2 47 5 and R 2 47 13 When mechanically aligning the connecting pieces and end connectors make sure that the field centering based on the main observer tube 2 47 4 is retained For this purpose select a conspicuous specimen area and place it in the intersec...

Page 66: ...40 075 e 10 03 1 Clamping screw 6 Coobservation tube 11 End connector R 2 Clamping screw 7 End connector L 12 Setting screw 3 Dovetail 8 Socket for power supply 13 Connecting piece R 4 Binocular tube 9 Plug 5 Connecting piece L 10 Power unit Fig 2 47 Attachment of multi observation equipment ...

Page 67: ...ction 2 48 2 on the stand and tighten clamping screw 2 48 10 only slightly at first Centering is performed using the dovetail Insert second clamping screw with washer 2 48 3 in the drilled hole provided on the rear of the intermediate tube at 2 48 4 screw it in the rear threaded hole of the adapter and tighten it slightly Align the intermediate tube with the edges of the upper stand part and alter...

Page 68: ...START UP Axioskop 2 plus Carl Zeiss Axioskop 2 mot plus 2 36 B 40 075 e 10 03 ...

Page 69: ...ight darkfield 3 27 3 3 3 Setting of transmitted light phase contrast 3 29 3 3 4 Setting of transmitted light polarization 3 31 3 3 5 Setting of transmitted light differential interference contrast DIC 3 36 3 3 6 Setting of epi fluorescence 3 39 3 4 Documentation 3 41 3 4 1 Attachment of photomicrography equipment 3 41 3 4 2 Attachment of videomicroscopy equipment 3 43 3 4 3 Attachment of adapter ...

Page 70: ...the height stop on the condenser carrier 3 26 Fig 3 19 Centering of darkfield stop on the universal condenser 3 28 Fig 3 20 Centering of phase stop on universal condenser 3 30 Fig 3 21 Centering of phase stop bright in condenser to phase ring dark in objective 3 30 Fig 3 22 Components for transmitted light polarization on the Axioskop 2 plus 3 31 Fig 3 23 Determination of the vibration direction n...

Page 71: ... a manual version and in a version with motorized coded components In the Operation chapter the manual setting and operation functions are explained first and the motorized coded functions are explained afterwards where necessary However many functions are identical in both versions Since the microscope software is only intended for the Axioskop 2 mot plus in combination with a PC it will be dealt...

Page 72: ... Operation and function controls Axioskop 2 mot plus 3 4 B 40 075 e 02 01 3 1 1 Operation and function controls of the Axioskop 2 plus Fig 3 1 Operation and function controls of the Axioskop 2 plus 1 0 2 0 3 0 4 0 5 0 6 0 7 0 8 0 90 10 0 ...

Page 73: ...ntensity 12 Color temperature key 3200 K 13 Focusing drive coarse adjustment on both sides 14 Focusing drive fine adjustment on both sides 15 Lever to swing in out the diffusion disk 16 Drive for movement of the mechanical stage in the y direction 17 Drive for movement of the mechanical stage in the x direction 18 3 levers to swing in out the filters in the transmitted light filter magazine 19 Cen...

Page 74: ...minous field diaphragm diameter see section 3 3 6 Pushrod pushed in fully open Pushrod pulled out closed Compartment for filter slider For 3 position or 6 position FL filter slider with 18 mm filter diameter Centering screws for aperture diaphragm incident light Centering of aperture diaphragm using SW 3 screwdriver see section 3 3 6 Pushrod for aperture diaphragm incident light Continuous setting...

Page 75: ...ated diffusion disk 3 1 15 is used to achieve homogeneous illumination though at a slight loss of light Lever in front position diffusion disk swung out Lever in rear position diffusion disk swung in Drive for movement of the mechanical stage in Y Movement of the mechanical stage in Y maximum travel range 50 mm Adjustable torque smoothness Drive for movement of the mechanical stage in X Movement o...

Page 76: ...erference contrast DIC I II III aperture diaphragm iris stop The front lens is folded in out using lever 3 2 2 Turning the turret wheel 3 2 4 swings the brightfield insert and the contrast stops in the beam path The abbreviation of the set turret position e g D is displayed in one of the windows 3 2 5 Slider 3 2 3 opens and closes the aperture diaphragm Mechanical stage with specimen holder mounti...

Page 77: ...ent light and optovar module and analyzer module for transmitted light Fast change of technique modules by turning the knurled ring of the reflector turret Luminous field diaphragm Wheel 3 5 2 for the continuous setting of the luminous field diaphragm transmitted light Additional mount 3 5 1 for filters with diameter d 32 mm Drive for condenser height adjustment Height adjustment of the condenser ...

Page 78: ...Carl Zeiss Operation and function controls Axioskop 2 mot plus 3 10 B 40 075 e 02 01 3 1 2 Overview of operation and function controls of the Axioskop 2 mot plus Fig 3 6 Operation and function controls of the Axioskop 2 mot plus ...

Page 79: ...e clamping ring Close the filter housing and tighten the knurled screw LED lamp voltage array Indicates the present operating voltage of the halogen illuminator in 10 steps 3 12 V Indicates the speed in 10 steps for the focusing in the programming mode Pilot lamp The LED pilot lamp for the line voltage lights up when the instrument is switched on During initialization of the instrument after switc...

Page 80: ... 3 7 1 aperture diaphragm is opened until stop as long as the key remains pressed Key close aperture diaphragm 3 7 2 aperture diaphragm is closed until stop as long as the key remains pressed Key front lens on off 3 7 3 front lens is folded in or out alternately Key turn turret disk to the right 3 7 4 turret disk is moved on to the right by one position To set the next position let go off the key ...

Page 81: ... key is activated the stage is moved downwards out of the focus position by approx 6 mm The current focus position is stored The specimen can be changed When the stage is in the low position the motorized focusing drive is switched off To avoid squashing of your fingers during fast stage lowering changing key the Axioskop 2 mot plus has been equipped with a safety switch off device The switch off ...

Page 82: ...y is activated the transmitted light is switched on or off Transmitted light is always available when the microscope is switched on Set key Stores illumination and condenser settings and is used for programming Binocular ergotube 6 25 23 The binocular ergotube 6 25 23 allows the viewing angle to be changed continuously in the range between 6 and 25 by swivelling the binocular component To set the ...

Page 83: ...epieces or to the attached camera via a pushrod Pushrod 3 10 2 pushed in 100 of the light for the eyepieces Pushrod 3 10 2 pulled out 100 of the light for the camera To set the most convenient viewing angle swivel the binocular component see 3 9 1 upwards or downwards The viewing height can be changed by turning the entire binocular component around 360 3 11 The interpupillary distance can be chan...

Page 84: ... 4400 when used individually or in a combination When several filters are combined the transmission value is calculated as the product of the individual transmission values in percent Filter slider The 3 position or 6 position FL filter slider 1 1 7 permits dia 18 mm filters e g PINKEL type filters to be inserted in the incident light beam path The position of the various filters in the beam path ...

Page 85: ...sed continuously from 60 to 110 mm This intermediate tube can only be used in combination with the binocular ergotube 6 25 23 or the binocular ergo phototube 6 25 23 100 100 Loosen clamping lever 3 13 2 of the tube A system of springs presses the upper part 3 13 1 of the tube upwards Press down upper part 3 13 1 or let go through the force of the spring until the required height is achieved Tighte...

Page 86: ...m check of all the connected components During the system check the pilot lamp on the stand lights up in orange After approx 3 seconds the microscope is ready for operation and is in the so called automatic mode The pilot lamp on the stand now lights up in green The Axioskop 2 mot plus features two different function modes and one programming mode Function modes In the automatic mode standard sett...

Page 87: ...ble beep is heard Settings Light intensity depending on objective nosepiece and technique module reflector turret Condenser turret position depending on objective nosepiece and technique module reflector turret Condenser front lens depending on objective nosepiece Aperture diaphragm opening depending on objective nosepiece Transmitted light illumination depending on technique module Set the Axiosk...

Page 88: ...denser turret Switching of condenser turret Folding in out of front lens Transmitted light on off Opening closing of aperture diaphragm transmitted light The values for illumination intensity and the condenser functions can be changed manually any time However if a new objective is swung in via the nosepiece or if a new reflector position is set the microscope control will automatically set the il...

Page 89: ...amming mode When the parameters for stage focusing are set individually the focus positions of the various objectives parfocality and the focusing speeds are matched to the appropriate objective magnification This is done in the programming mode which is reached by pressing the Set key for more than 2 seconds The focus positions and focusing speeds are normally matched to the objective magnificati...

Page 90: ...n 10 steps via the two keys for fast stage lowering lifting 3 14 2 Key higher speed Key lower speed The focusing speed can be matched to the individual requirements by switching between coarse and fine adjustment 3 14 3 and by experimenting The set speed is indicated on the LED array on the right of the stand 3 16 1 Then precisely focus on the specimen again and turn the nosepiece to bring the obj...

Page 91: ...ng mode is confirmed by a beep when the Set key is released The pilot lamp lights up in green again If several reflector positions are to be used e g different reflector modules for incident light or Optovar modules for transmitted light parfocality must be set and focusing speed matched in the programming mode separately for each reflector position in accordance with the steps described above ...

Page 92: ... to KÖHLER on the manual Axioskop 2 plus 2 Transmitted light brightfield equipment of the manual Axioskop 2 plus Each manual Axioskop 2 plus configuration permits performance of the transmitted light brightfield technique 3 Transmitted light brightfield settings according to KÖHLER on the manual Axioskop 2 plus The manual Axioskop 2 plus has been set up as described in chapter 2 The manual Axiosko...

Page 93: ...k into the tube with your naked eye Use sliding knob 3 17 5 to set the aperture diaphragm to approx 2 3 4 5 of the diameter of the objective exit pupils 3 17 E In most applications this aperture diaphragm setting provides optimum contrast at almost ideal resolution and is therefore the best compromise for the human eye Insert eyepiece in the tube again Since field size and objective aperture chang...

Page 94: ...rs the matching of focus positions and focus speeds of the used objectives has been finished The instrument is switched on and is in the automatic mode Illumination intensity condenser settings position of the front lens position of the turret disk and the opening of the aperture diaphragm and the status transmitted light switched on or off are stored by pressing the Set key Setting of KÖHLER illu...

Page 95: ...ield settings on the manual Axioskop 2 plus and Axioskop 2 mot plus Setting of KÖHLER illumination is identical to transmitted light brightfield except that the highest aperture objective must be used instead of the 10x objective Turn turret disk of the universal condenser to position D for Axioskop 2 mot plus using key 3 7 4 or 5 and swing in condenser front lens for Axioskop 2 mot plus using key...

Page 96: ...d and the luminous field diaphragm image will be almost perfectly in focus Finally match the diameter of the luminous field diaphragm to the size of the field of view Darkfield microscopy requires specimens to be considerably cleaner than in other techniques Finger prints dirt or dust particles in particular have negative effects since they brighten the background of the field of view and decrease...

Page 97: ...is optical channel and are influenced by the in phase refractive index and the thickness differences in the specimen In the intermediate image plane the differently influenced partial beams interfere and are enhanced or attenuated depending on the phase position This interference results in image contents displaying intensity and color differences which can be recognized by the human eye 2 Configu...

Page 98: ...If congruence is not perfect 3 21 A the two SW 1 5 screwdrivers 3 20 1 and 4 must be used on the two centering screws 3 20 2 and 3 to recenter the bright phase stop until complete congruence with the dark phase ring is achieved 3 21 B Insert eyepiece in the tube again Normally however centering is not required since the phase stops are factory centered and the centering is retained even if the uni...

Page 99: ...guration Objectives suitable for polarization Rotary mechanical stage 75x50 240 R SENARMONT DIC polarizer or polarizer D rotary or fixed unless a polarizer is integrated in the condenser Analyzer module D in the reflector turret 3 Setting of Axioskop 2 plus and Axioskop 2 mot plus Set the microscope as for transmitted light brightfield according to KÖHLER see section 3 3 1 3 Center rotary mechanic...

Page 100: ... rotary or fixed unless a condenser with integrated polarizer is used Analyzer module D in reflector turret Adjusting specimen for polarization microscopy 453679 0000 000 3 Setting of Axioskop 2 plus and Axioskop 2 mot plus Set the microscope as for transmitted light brightfield see section 3 3 1 3 taking care to ensure that the interpupillary distance of the binocular tube has been correctly set ...

Page 101: ...argest vibration direction nγ defined to be oriented in NE SW direction The specimen changes its color when the compensator λ is pushed in The type of the color change depends on the orientation of the specimen NE SW or NW SE The color changes are caused by optical interference The interference colors path differences in both diagonal positions NE SW and NW SE of the specimen must be compared The ...

Page 102: ...men to be examined is parallel to the largest vibration direction of the compensator λ i e in NE SW direction the path difference of the specimen e g grayish white 150 nm and the path difference of the compensator λ red 550 nm add up This results in a color change of the specimen from grayish white to greenish blue resulting path difference 700 nm If the vibration direction of the specimen to be e...

Page 103: ... of Axioskop 2 plus and Axioskop 2 mot plus Set the microscope as for transmitted light brightfield according to KÖHLER see section 3 3 1 3 Center the rotary stage 3 25 3 see section 2 2 9 2 and then remove specimen from the stage Swing circular polarizer 3 25 2 in the beam path Swing analyzer module D on reflector turret 3 25 4 The field of view now appears dark on account of the crossed polarize...

Page 104: ...ioskop 2 mot plus configuration Objectives offered with DIC equipment e g Plan Neofluar Nosepiece with compartment for DIC slider DIC slider suitable for the objectives used Condenser with turret disk with DIC prisms DIC I DIC II DIC III Polarizer e g SENARMONT polarizer In the case of the achromatic aplanatic universal condensers 0 9 H D Ph DIC 445439 or 445440 MOT the DIC prisms in the turret di...

Page 105: ...ning the polarizer via the lever in the lower part of the SENARMONT polarizer 3 26 7c The λ 4 plate above it must always be switched on for SENARMONT contrasting to function If the DIC slider was moved to the center position before there will be darkness in the stop position of the SENARMONT polarizer i e the path difference amounts to 0 nm in this position If required the compensator λ 3 26 6 can...

Page 106: ...6 0 7 0 8 0 90 10 0 1 Objective in nosepiece 7a DIC prism with polarization foil 2 Reflector turret in the condenser or 3 Analyzer module 7b D polarizer 4 DIC slider 7c SENARMONT polarizer 5 Knurled screw 8 Sliding knob for aperture 6 compensator λ diaphragm Fig 3 26 Components for the transmitted light DIC technique on the Axioskop 2 plus ...

Page 107: ... UV excitation FL P C reflector module and shutter plate in the reflector turret HBO 103 or HBO 50 mercury vapor short arc lamp for incident light illumination HAL 100 halogen illuminator for transmitted light illumination Before the epi fluorescence technique is applied it is absolutely necessary to adjust the mercury vapor short arc lamp in accordance with section 2 1 11 by using the adjusting a...

Page 108: ...ed Insert eyepiece in tube again and close luminous field diaphragm 3 27 5 until it is visible in the field of view Use the two centering screws 3 27 6 to center the luminous field diaphragm in relation to the edge of the field of view Either open the luminous field diaphragm until it just disappears behind the edge of the field of view or if there is a risk of specimen bleaching reduce it until i...

Page 109: ...t of a SLR camera e g CONTAX 167 MT Screw T2 adapter 416010 0000 000 3 28 3 suitable for the used camera on the 2 5x connector for T2 456005 0000 000 3 28 4 Attach camera 416181 0000 000 3 28 2 and if required cable release 416167 0000 000 3 28 1 Remove dust cap 3 28 8 from the phototube 3 28 5 and insert premounted unit A in the phototube Align unit in the required position and tighten 3 Allen sc...

Page 110: ... cameras T2 Adapter for SLR cameras Cat No T2 Adapter for CONTAX CONTAX bayonet 416010 0000 000 T2 Adapter for OLYMPUS OM OM bayonet 416002 0000 000 T2 Adapter for MINOLTA SR bayonet 416003 0000 000 T2 Adapter for CANON FD bayonet 416004 0000 000 T2 Adapter for NIKON F bayonet 416009 0000 000 T2 Adapter for PENTAX KA bayonet 416011 0000 000 ...

Page 111: ...gh resolution CCD cameras to the phototube of the Axioskop 2 plus Axioskop 2 mot plus Tube Adapters Cameras 456105 456105 0000 000 60 C 2 3 1 0 456107 000000 1069 414 60 C 2 3 0 63 456106 456106 0000 000 60 C 1 2 0 5 456108 456108 0000 000 60 C 1 3 0 4 452989 0000 000 Zoom 44 C 1 3 3 CCD 0 35x 1 6x To be used with adapter 60 44 456140 0000 000 456115 456115 0000 000 60 ENG 2 3 1 0 456124 452984 00...

Page 112: ...140 456140 0000 000 Adapter 60 44 Microscopes with 60 mm interface and all video adapters for 44 mm interface Connects video adapters for 44 mm interface to microscopes with 60 mm interface 3 4 3 Attachment of adapter for digital compact cameras Tube Adapters Cameras 000000 1096 522 D40 M52x0 75 To be used with 456006 0000 000 000000 1108 984 44 M52x0 75 To be used with 456140 0000 000 PHOTO TUBE ...

Page 113: ...e 6 25 23 3 29 1 or binocular ergo phototube 6 25 23 to the front tube mount of the microscope Attach binocular tube 3 29 2 or phototube 30 23 to the rear tube mount of the microscope The binocular ergotube 6 25 23 or phototube provides an upright unreversed image in the eyepiece For technical reasons the epi fluorescence technique cannot be applied in combination with the active coobservation equ...

Page 114: ...ipment Axioskop 2 mot plus 3 46 B 40 075 e 02 01 1 Binocular ergotube 2 Binocular tube 3 Basic body 4 Power unit 5 Knob for brightness control 6 Lever for light pointer Fig 3 29 Active coobservation equipment for 2 users 1 0 2 0 3 0 4 0 5 0 6 0 7 0 8 0 90 10 0 ...

Page 115: ...nnector L 3 30 8 left Binocular tubes 30 23 for further viewing stations 3 30 6 and 7 452927 0000 000 We would recommend you to have the multi observation equipment assembled by Carl Zeiss service staff since careful optical adjustment and centering of the fields of view of the various observation tubes is required Should you want to perform assembly yourself see section 2 2 17 Attachment of multi...

Page 116: ...8 B 40 075 e 02 01 1 Knob for brightness control 7 Coobservation tube 2 Basic body 8 End connector L 3 Lever for light pointer 9 Power unit 4 Binocular tube 10 End connector R 5 Connecting piece L 11 Connecting piece R 6 Coobservation tube Fig 3 30 Attachment of multi observation equipment ...

Page 117: ...he microscope This enables the user to copy microscope specimens by drawing Furthermore transparent references e g grain size plates reference scales for photomicrography or other self made patterns with bright lines on a dark background can be laid on a light box and projected into the microscope image On account of its good imaging performance the intermediate tube can also be combined with a mi...

Page 118: ...is scale value is exactly that distance in the specimen which complies to one interval of the used crossline micrometer For calibration align the scales of the stage micrometer and the crossline micrometer parallel to each other by turning the eyepiece and make the zero line of both scales exactly congruent If for example 99 increments of 10 µm each of the stage micrometer correspond to exactly 10...

Page 119: ...ments using the microscope are always possible to be performed if both the lower and upper side of the specimen can be focused This should preferably be performed using a precision Harmonic Drive focusing drive and a high aperture objective with a low depth of focus The difference in the stage height results in a height value for transmitted light specimens which is falsified by the refractive ind...

Page 120: ...y objective nM 1 0 The indicated intervals of the mechanical fine drive are 15 0 and 24 5 which means that d 9 5 µm with an interval of 1 µm The refractive index of the specimen area nP has been assumed as nP 1 5 Therefore the thin section height is d d x 9 5 µm x 14 25 µm 10 20 3 0 40 5 0 6 0 7 0 8 0 90 1 0 0 1 0 20 30 4 0 50 6 0 7 0 8 0 9 0 100 Fig 3 33 Height measurement nP nM 1 5 1 ...

Page 121: ...AINTENANCE TROUBLESHOOTING AND SERVICE Contents 4 CARE MAINTENANCE TROUBLESHOOTING AND SERVICE 4 2 4 1 Instrument care 4 2 4 2 Instrument maintenance 4 3 4 2 1 Performing checks 4 3 4 2 2 Changing the fuses 4 3 4 3 Troubleshooting 4 4 4 4 Spares consumables and tools 4 8 4 5 Requesting service 4 9 List of illustrations Fig 4 1 Changing the fuses 4 3 ...

Page 122: ...e Optics cleaning solution consisting of 15 isopropanol and 85 medical alcohol benzoline clean glass surface using a slightly moistened wad of cotton by moving in circles starting in the middle Remove dust from optical surfaces using a natural hair brush or an air blower When using the microscope in humid climatic zones proceed as follows Store instrument in bright dry and well ventilated rooms wi...

Page 123: ...ake sure that the maximum operation time of the halogen and mercury vapor short arc lamps is not exceeded 4 2 2 Changing the fuses Always pull the power plug before changing the fuses The fuse compartment is positioned at the microscope rear It is combined with the instrument socket and contains two T4 A H 250 V fuses Disconnect the instrument from the line Pull out the fuse holder 4 1 1 from the ...

Page 124: ...n the phototube in the correct position end position see page 3 15 visible Nosepiece with objective not in click stop position Switch nosepiece with objective in click stop position Condenser not set correctly Set condenser correctly adjustment centering see p 3 24 ff Aperture diaphragm not set correctly Set aperture diaphragm correctly centering opening see p 3 24 ff Luminous field diaphragm not ...

Page 125: ... Use of no or unspecified immersion oil with immersion objectives Use immersion oil 518 N from Carl Zeiss see p 4 8 Air bubbles in the immersion oil Apply new oil to remove the bubbles Immersion oil at the front lens of a dry objective Clean the front lens of the dry objective see p 4 2 Corr ring is not set to the correct cover slip thickness Set the corr ring to the correct thickness see p 1 12 D...

Page 126: ...te ametropia s p 2 5 Eye fatiguing microscopy Interpupillary distance of the binocular tube is not set correctly Set interpupillary distance correctly see p 2 6 Focusing eyepieces are not set correctly Set focusing eyepieces to the appropriate ametropia s p 2 5 Image brightness not acceptable Adjust lamp voltage or insert conversion filter Binocular tube optically mechanically out of alignment Che...

Page 127: ...stop nosepiece in the required position The 12 V 100 W halogen lamp of the Axioskop 2 mot plus does not function although the on off switch is in on position Reflector turret is in epi fluorescence positions where the transmitted light off status is stored Set the correct reflector turret position or switch on transmitted light illumination using the transmitted light on off key on the left of the...

Page 128: ...p 444801 0000 000 recommended for low brightness techniques to suppress reflected light Dust cover for nosepiece Dust cover for eyepiece tube 462981 0000 000 000000 0168 373 to close instrument openings which are not used 518 N immersion oil 20 ml oiler 100 ml bottle 250 ml bottle 500 ml bottle 444950 0000 000 444952 0000 000 444953 0000 000 444954 0000 000 for oil immersion applications nD 1 515 ...

Page 129: ... Axioskop 2 mot plus may only be performed by Carl Zeiss service staff or specially authorized personnel To ensure the optimum setting and trouble free function of your microscope even for a longer period of time we would recommend you to conclude a service maintenance contract with Carl Zeiss In the case of subsequent orders or when service is required please get in touch with your local Zeiss ag...

Page 130: ...CARE MAINTENANCE TROUBLESHOOTING AND SERVICE Axioskop 2 plus Carl Zeiss Axioskop 2 mot plus 4 10 B 40 075 e 10 03 ...

Page 131: ...Axioskop 2 plus ANNEX Axioskop 2 mot plus Overview Carl Zeiss B 40 075 e 10 03 A 1 ANNEX List of abbreviations A 2 List of key words A 4 Patent rights A 8 ...

Page 132: ...tage height setting focus difference in height measurement DC Direct Current DIC Differential Interference Contrast DIN Deutsches Institut für Normung German standards association DL transmitted light DX coding system for the storage of electronically legible information e g film speed EG European Community EN European standards EMV electromagnetic compatibility ENG Electronics News Gathering ERGO...

Page 133: ...e index of the medium nD refractive index of D line sodium nP refractive index of the specimen Ph phase contras PL flatfield R right drive on the right of the mechanical stage SLR Single Lens Reflex SW wrench opening T slow blow fuse type TV television T2 Adapter standardized connector for 35 mm cameras UL Underwriter Laboratories UV ultraviolet VDE Verband Deutscher Elektrotechniker association o...

Page 134: ... 4 3 6 3 14 Binocular ergo phototube 3 15 Birefringence 3 31 Brightfield 3 8 3 20 3 24 3 27 3 29 C Changing the fuses 4 3 Coarse 3 5 3 11 3 22 Coarse drive 3 7 Color temperature key 3200 K 3 7 Condenser 1 2 1 3 2 3 2 7 2 15 3 5 3 7 3 20 Condenser carrier 2 7 2 15 3 26 Condenser height adjustment 3 9 Consumables 4 8 Conversions 2 15 Coobservation equipment 2 31 3 45 D Darkfield 3 27 D polarizer 2 1...

Page 135: ...module 2 29 Fluorescence 3 39 Focus positions 1 3 3 21 3 26 Focusing drive 3 5 3 9 3 11 3 13 3 26 Focusing drive 3 7 Front lens 1 3 2 7 2 15 3 8 3 12 3 24 3 26 Function modes 3 18 3 19 G Gear switchover 3 11 3 13 H Halogen illuminator 2 9 3 6 3 11 Halogen lamp 2 19 Harmonic Drive TM 1 3 HBO 103 illuminator 2 10 Height measurement 3 51 Height stop on condenser 3 26 I Illumination and contrasting te...

Page 136: ... 25 3 30 3 40 M Maintenance 4 3 Manual mode 3 18 3 21 Measurement of lengths 3 50 Mechanical stage 2 3 2 22 2 25 3 5 3 7 3 8 Mercury vapor short arc lamp 2 11 3 40 Modules 1 4 1 8 Multi observation equipment 2 33 3 45 3 47 N Nosepiece 2 6 3 5 3 9 3 11 3 12 3 20 3 22 3 25 O Objectives 2 6 2 3 2 23 Objective nosepiece 1 3 Objective nosepiece 1 18 On off switch 3 9 Operation 1 16 Operation and functi...

Page 137: ... 20 3 22 3 23 3 26 Smoothness 2 24 2 25 3 8 Spares 4 8 Specimen holder 2 3 2 25 Stage carrier 2 15 2 22 2 23 2 7 Stage focusing 1 18 3 9 3 13 3 21 3 22 Start up 2 1 2 3 Switching on 3 18 Switch on switch off 2 8 T Technical Data 1 16 Technique modules 2 3 3 9 3 13 Torque 2 24 Tools 4 8 Transmitted light 3 9 3 11 3 13 3 20 3 24 3 26 3 27 3 29 3 31 3 35 3 36 Transmitted light filter magazine 3 16 Tu...

Page 138: ...nt rights Axioskop 2 mot plus A 8 B 40 075 e 10 03 Patent rights The instruments instrument components or methods described in this manual are protected by the following patents DE19828548 US6327080 DE29821694 US6392796 US5015082 JP2532164 ...

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