Zeiss AxioObserver D1, User Manual

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4. Repeat exposure time measurement for each channel, however, adjust the fine focus for only one
channel ie the most important one
5. To capture your final image, press the button located at the bottom of the C tab.
Post ‐ Acquisition Adjustments
Brightness/Contrast
1. Temporarily turn off the pseudo ‐ coloring of the displayed image by pressing the button. Each
channel is displayed in black/white; you can view one channel at a time.
2. Click on the properties button found below the image
3. While under Display tab of properties window, click the Min/Max button, or under your image, press
the button.
4. You can make further adjustments using the displayed histogram (ie move top of line left, and bottom
of line right)
‐ NOTE: do not adjust the Gamma
5. Repeat for each channel
6. Press the button
Adding a Scale bar
1. To add a scale bar , press the button, or Annotations from the main menu and select Scale bar.
2. Drag cursor along image to get desired scale bar size
Saving Files
1. From the main menu, select File

Save as
2. Save your files in the AxioVision format (.zvi) in the appropriate destination

your lab folder

your
folder. Please refer to the Attention Users signs posted on the wall.
Shut Down Procedure
8 If used oil, gently remove oil from objective using neatly folded dry lens paper
9 Put 10x objective in place, and lower objective
10 Close AxioVision program. Do not save changes made to the experiment!
11 Shut down computer. Wait until completely turned off
12 Turn Microscope OFF
13 Toggle Power Bar switch OFF
14 FluoArc Lamp :
‐ Regular work hours : set intensity to 50% on control pad (F2), and leave lamp ON
‐ Non ‐ work hours : Turn lamp OFF
15 Cover microscope with dust cover, but DON’T COVER THE HOT LAMP HOUSING!!