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Attention

 

Users!!

 

 

FluoArc

 

Lamp

:

 

 

The

 

FluoArc

 

lamp

 

must

 

be

 

on

 

for

 

at

 

least

 

30

 

minutes

 

before

 

being

 

turned

 

off

 

AND

 

must

 

not

 

be

 

turned

 

on

 

again

 

within

 

1

 

hour

 

of

 

being

 

turned

 

off.

  

1.

 

When

 

starting,

 

before

 

you

 

turn

 

on

 

the

 

FluoArc

 

lamp,

 

make

 

sure

 

it

 

has

 

been

 

OFF

 

for

 

at

 

least

 

1

 

hour

 

from

 

previous

 

use.

 

2.

 

When

 

finishing,

 

before

 

you

 

turn

 

off

 

the

 

FluoArc

 

lamp,

 

make

 

sure

 

it

 

has

 

been

 

ON

 

for

 

at

 

least

 

30

 

minutes.

 

 

Oil

 

on

 

Objectives

:

 

 

If

 

you

 

used

 

an

 

oil

 

objective,

 

please

 

remember

 

to

 

wipe

 

oil

 

off

 

with

 

dry

 

Lens

 

Paper.

 

Do

 

not

 

use

 

ethanol!

 

 

Booking

 

Time:

 

 

You

 

are

 

allowed

 

to

 

sign

 

up

 

for

 

a

 

maximum

 

of

 

3

 

hours

 

per

 

day.

 

Please

 

make

 

sure

 

you

 

only

 

book

 

time

 

that

 

you

 

need.

 

You

 

are

 

responsible

 

for

 

showing

 

up

 

for

 

your

 

time

 

booked

.

 

A

 

$10.00

 

fee

 

applies

 

if

 

you

 

did

 

not

 

show

 

up

 

for

 

booked

 

time.

 

 

Log

 

Book:

 

 

All

 

users

 

must

 

log

 

the

 

time

 

spent

 

on

 

the

 

station

 

as

 

well

 

as

 

the

 

time

 

the

 

fluorescence

 

lamp

 

was

 

turned

 

on/off

 

in

 

the

 

log

 

book

.

  

 

Save

 

Your

 

Data

:

 

 

Save

 

all

 

of

 

your

 

work

 

in

 

D:/

 

drive,

 

your

 

lab

folder,

 

your

 

folder

.

 

Files

 

found

 

on

 

the

 

Desktop

 

or

 

in

 

the

 

C:/

 

drive

 

will

 

be

 

deleted

 

daily.

 

 

You

 

are

 

responsible

 

for

 

backing

up

 

your

 

own

 

data

 

at

 

all

 

times.

 

Do

 

not

 

rely

 

on

 

this

 

computer

 

as

 

storage

 

for

 

your

 

data.

 

Once

 

you

 

have

 

backed

up

 

your

 

files

 

elsewhere,

 

please

 

delete

 

your

 

files

 

from

 

this

 

computer.

 

Summary of Contents for AxioObserver D1

Page 1: ......

Page 2: ...Open Program AxioVision Rel 4 8 Shut Down Procedure 1 If used oil gently remove oil from objective using neatly folded dry lens paper 2 Put 10x objective in place and lower objective 3 Close AxioVisi...

Page 3: ...hat channels you want to image ie All Channels 4 Select the colors tab 5 You can activate deactivate channels by right mouse clicking on the channel number Set Sample on Stage and Focus Only use slide...

Page 4: ...each channel 6 Press the button Adding a Scale bar 1 To add a scale bar press the button or Annotations from the main menu and select Scale bar 2 Drag cursor along image to get desired scale bar size...

Page 5: ...hod HAL Lamp Voltage TL Shutter open RL Shutter closed Reflector Located on bottom front of microscope Controls HAL lamp voltage intensity Diaphragm Control Condenser contrast method Condenser apertur...

Page 6: ...r Correction UV Transmission IR Transmission DIC DIC II Phase Contrast Ph1 Ph2 Catalog 441031 0000 000 421351 9970 710 420650 9901 00 4 5 6 Plan NeoFluar 40x Plan Apochromat 63x Plan NeoFluar 5x 1 3 O...

Page 7: ...Plan NeoFluar 100x 1 3 Oil Ph3 100x 1 3 0 2 0 17 Oil ICS Ph3 000000 1031 171...

Page 8: ...sion BP 460 50 3 Filter Set 38 Cy2 GFP Excitation BP 470 40 Beam Splitter FT 495 Emission BP 525 50 4 Filter Set 46 HE YFP Excitation BP 500 25 Beam Splitter FT 515 Emission BP 535 30 5 Filter Set 45...

Page 9: ...Filter set...

Page 10: ......

Page 11: ...https www micro shop zeiss com p us f f...

Page 12: ...g Time You are allowed to sign up for a maximum of 3 hours per day Please make sure you only book time that you need You are responsible for showing up for your time booked A 10 00 fee applies if you...

Page 13: ...load your sample on the microscope stage with the objective lowered to the lowest focus position Oil should never reach the outer rim of the objectives The felt protectors should never become saturate...

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