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Zeiss

 

AxioObserver.D1

 

Microscope

 

 

 

 

 

 

 

 

 

 

 

 

 

Room:

 

3164A

 

 

 

Start

 

Up

 

Procedure

 

1.

 

Remove

 

dust

 

cover

 

from

 

microscope

 

2.

 

Toggle

 

FluoArc

 

Lamp

 

switch

 

ON

 

3.

 

Toggle

 

Power

 

Bar

 

switch

 

ON

 

4.

 

Turn

 

Microscope

 

ON

 

 

by

 

pressing

 

power

 

button

 

located

 

on

 

left

 

side

 

of

 

microscope

 

5.

 

Turn

 

ON

 

computer

 

6.

 

Log

 

on

 

to:

 

Observer

 

User

 

account

 

(

no

 

password

)

 

7.

 

Open

 

Program:

 

AxioVision

 

Rel.

 

4.8

 

 

Shut

 

Down

 

Procedure

 

 

1.

 

If

 

used

 

oil,

 

gently

 

remove

 

oil

 

from

 

objective

 

using

 

neatly

 

folded

 

dry

 

lens

 

paper

 

2.

 

Put

 

10x

 

objective

 

in

 

place,

 

and

 

lower

 

objective

 

3.

 

Close

 

AxioVision

 

program

 

4.

 

Shut

 

down

 

computer.

 

Wait

 

until

 

completely

 

turned

 

off

  

5.

 

Turn

 

Microscope

 

OFF

 

6.

 

Toggle

 

Power

 

Bar

 

switch

 

OFF

 

7.

 

FluoArc

 

Lamp

:

 

 

Regular

 

work

 

hours

:

 

set

 

intensity

 

to

 

50%

 

on

 

control

 

pad

 

(F2),

 

and

 

leave

 

lamp

 

ON

 

 

Non

work

 

hours

:

 

Turn

 

lamp

 

OFF

 

8.

 

Cover

 

microscope

 

with

 

dust

 

cover,

 

but

 

DON’T

 

COVER

 

THE

 

HOT

 

LAMP

 

HOUSING!!

 

 

Summary of Contents for AxioObserver D1

Page 1: ......

Page 2: ...Open Program AxioVision Rel 4 8 Shut Down Procedure 1 If used oil gently remove oil from objective using neatly folded dry lens paper 2 Put 10x objective in place and lower objective 3 Close AxioVisi...

Page 3: ...hat channels you want to image ie All Channels 4 Select the colors tab 5 You can activate deactivate channels by right mouse clicking on the channel number Set Sample on Stage and Focus Only use slide...

Page 4: ...each channel 6 Press the button Adding a Scale bar 1 To add a scale bar press the button or Annotations from the main menu and select Scale bar 2 Drag cursor along image to get desired scale bar size...

Page 5: ...hod HAL Lamp Voltage TL Shutter open RL Shutter closed Reflector Located on bottom front of microscope Controls HAL lamp voltage intensity Diaphragm Control Condenser contrast method Condenser apertur...

Page 6: ...r Correction UV Transmission IR Transmission DIC DIC II Phase Contrast Ph1 Ph2 Catalog 441031 0000 000 421351 9970 710 420650 9901 00 4 5 6 Plan NeoFluar 40x Plan Apochromat 63x Plan NeoFluar 5x 1 3 O...

Page 7: ...Plan NeoFluar 100x 1 3 Oil Ph3 100x 1 3 0 2 0 17 Oil ICS Ph3 000000 1031 171...

Page 8: ...sion BP 460 50 3 Filter Set 38 Cy2 GFP Excitation BP 470 40 Beam Splitter FT 495 Emission BP 525 50 4 Filter Set 46 HE YFP Excitation BP 500 25 Beam Splitter FT 515 Emission BP 535 30 5 Filter Set 45...

Page 9: ...Filter set...

Page 10: ......

Page 11: ...https www micro shop zeiss com p us f f...

Page 12: ...g Time You are allowed to sign up for a maximum of 3 hours per day Please make sure you only book time that you need You are responsible for showing up for your time booked A 10 00 fee applies if you...

Page 13: ...load your sample on the microscope stage with the objective lowered to the lowest focus position Oil should never reach the outer rim of the objectives The felt protectors should never become saturate...

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