background image

 

Computer:

 

 

This

 

computer

 

is

 

to

 

be

 

used

 

only

 

for

 

image

 

acquisition.

 

No

 

other

 

work

 

is

 

permitted!!

 

 

Immersion

 

Oil

 

Rules:

 

 

 

Always

 

keep

 

immersion

 

oil

 

bottle

 

closed

.

 

This

 

prevents

 

water

 

evaporation

 

and

 

maintains

 

the

 

integrity

 

of

 

the

 

oil

 

 

Keep

 

oil

 

bottle

 

clean

.

 

Oil

 

should

 

never

 

be

 

found

 

along

 

the

 

sides

 

of

 

the

 

bottle

 

or

 

around

 

the

 

neck

 

 

Apply

 

only

 

a

 

small

 

amount

 

of

 

oil

 

to

 

your

 

coverslip

 

 

 

 

 

Always

 

load

 

your

 

sample

 

on

 

the

 

microscope

 

stage

 

with

 

the

 

objective

 

lowered

 

to

 

the

 

lowest

 

focus

 

position

 

 

Oil

 

should

 

never

 

reach

 

the

 

outer

 

rim

 

of

 

the

 

objectives.

 

The

 

felt

 

protectors

 

should

 

never

 

become

 

saturated

 

with

 

oil

 

 
 
 

 

 

Always

 

remove

 

oil

 

using

 

dry

 

Lens

 

Paper

 

from

 

the

 

objective

 

when

 

changing

 

slides.

 

Re

apply

 

a

 

small

 

amount

 

of

 

new

 

oil

 

to

 

the

 

new

 

slide

 

 

If

 

you

 

are

 

imaging

 

a

 

sample

 

using

 

an

 

oil

 

objective,

 

do

 

not

 

toggle

 

between

 

objectives

.

 

Image

 

with

 

that

 

objective

 

only!

 

 

When

 

done,

 

thoroughly

 

wipe

 

off

 

oil

 

objective

 

using

 

Lens

 

Paper

  

 

Do

 

not

 

get

 

oil

 

on

 

any

 

air

 

objectives!

 

 

 

Coverslip

Oil

 

droplet

Summary of Contents for AxioObserver D1

Page 1: ......

Page 2: ...Open Program AxioVision Rel 4 8 Shut Down Procedure 1 If used oil gently remove oil from objective using neatly folded dry lens paper 2 Put 10x objective in place and lower objective 3 Close AxioVisi...

Page 3: ...hat channels you want to image ie All Channels 4 Select the colors tab 5 You can activate deactivate channels by right mouse clicking on the channel number Set Sample on Stage and Focus Only use slide...

Page 4: ...each channel 6 Press the button Adding a Scale bar 1 To add a scale bar press the button or Annotations from the main menu and select Scale bar 2 Drag cursor along image to get desired scale bar size...

Page 5: ...hod HAL Lamp Voltage TL Shutter open RL Shutter closed Reflector Located on bottom front of microscope Controls HAL lamp voltage intensity Diaphragm Control Condenser contrast method Condenser apertur...

Page 6: ...r Correction UV Transmission IR Transmission DIC DIC II Phase Contrast Ph1 Ph2 Catalog 441031 0000 000 421351 9970 710 420650 9901 00 4 5 6 Plan NeoFluar 40x Plan Apochromat 63x Plan NeoFluar 5x 1 3 O...

Page 7: ...Plan NeoFluar 100x 1 3 Oil Ph3 100x 1 3 0 2 0 17 Oil ICS Ph3 000000 1031 171...

Page 8: ...sion BP 460 50 3 Filter Set 38 Cy2 GFP Excitation BP 470 40 Beam Splitter FT 495 Emission BP 525 50 4 Filter Set 46 HE YFP Excitation BP 500 25 Beam Splitter FT 515 Emission BP 535 30 5 Filter Set 45...

Page 9: ...Filter set...

Page 10: ......

Page 11: ...https www micro shop zeiss com p us f f...

Page 12: ...g Time You are allowed to sign up for a maximum of 3 hours per day Please make sure you only book time that you need You are responsible for showing up for your time booked A 10 00 fee applies if you...

Page 13: ...load your sample on the microscope stage with the objective lowered to the lowest focus position Oil should never reach the outer rim of the objectives The felt protectors should never become saturate...

Reviews: