![background image](http://html2.mh-extra.com/html/zeiss/axiolab-5/axiolab-5_operating-manual_3357225095.webp)
OPERATION
Axiolab 5
Illumination and contrast methods in transmitted light
ZEISS
05/2019
430037-7444-001
95
−
The suitable compensator is:
K 0-30
λ
tilting compensator
(Accessory 000000-1115-698)
−
A dark gray appearing as the interference color indicates a very small path difference (
λ
/10 or
54.6 nm).
•
Insert the compensator into the slot as far as it will go.
The accompanying instructions must be observed for the measurement preparation and procedure.
4.2.4.4
Circular polarization contrast
(1) Application
Unlike standard polarization contrast, circular polarization contrast does not show any dark (extinction)
positions that depend on the angle of rotation (azimuth) of the specimen relative to the polarizer or
analyzer. This means that when the stage is rotated the same image impression remains, as the light/dark
positions are omitted. With optical anisotropy, all transparent specimens display the characteristic
interference colors.
(2) Instrumentation
−
Strain-free objectives
−
Pol rotary stage
−
Circular polarizer D (no polarizers may be adapted on the condenser) including corresponding
lambda/4 plate.
−
Stationary analyzer slider D or screw-in analyzer (in Axiolab 5 tubes).
(3) Configuring the microscope
•
Set the microscope as for transmitted light brightfield microscopy using the KÖHLER method (see
section 4.2.1).
•
Center the Pol rotary stage or objective (if this has not already been done – see sections 3.1.7.5
and 3.1.7.6).
•
Initially do
not
use a specimen for the further settings.
•
Place the analyzer in position.
•
Swivel the lower part of the circular polarizer D (Fig. 4-10/
2
) into the beam path until it engages and
evaluate the extinction (darkening) of the field of view without the specimen at full light intensity.
If this is not optimal, align the analyzer in the tube or intermediate plate as necessary.
•
Insert the respective slider 6x20mm with the lambda/4 plate (Fig. 4-10/
1
) as far as it will go into the
slot for compensators above the nosepiece.