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OPERATION
Axiolab 5
Illumination and contrast methods in reflected light
ZEISS
05/2019
430037-7444-001
117
(3) Configuring reflected light fluorescence
The adjustment of reflected light fluorescence is facilitated by starting with an objective of average
magnification, e.g. EC Plan-Neofluar 20x/0.50, and a specimen of high fluorescence. Demonstration
samples can also be used for the start-up.
NOTE
If the lambda compensator used for the transmitted light polarization method is still in its slot
above the nosepiece, it must be removed before configuring reflected light fluorescence.
•
Slide the fluorescence protection shield (Fig. 4-20/
8
) into the compensator slot above the nosepiece.
•
Swivel in the 20x/0.50 EC Plan-Neofluar objective on the nosepiece (Fig. 4-20/
2
).
•
Initially set transmitted light illumination by pushing the
TL
button (Fig. 4-20/
5
).
•
If necessary, turn the condenser turret (Fig. 4-20/
7
) to the
BF
position for transmitted light brightfield
(or phase contrast if using a Ph objective) and seek the specimen detail to be examined.
•
Adjust the light intensity by turning the Intensity/LM knob (Fig. 4-20/
3
) and focus (Fig. 4-20/
6
).
•
On the reflector turret (Fig. 4-20/
9
), select the FL P&C reflector module with the desired fluorescence
filter combination (depending on the excitation mode) and swivel it into the beam path.
•
Use the LED selection knob (Fig. 4-20/
1
) to swivel the desired LED (UV, B or G) into the beam path.
ATTENTION
To avoid dazzling when switching between the LEDs, the brightness should be reduced
slightly beforehand.
NOTE
When switching among the three LEDs, the current brightness setting is adopted.
•
Switch on reflected light illumination by pushing the
RL
button (Fig. 4-20/
4
).
•
Adjust the light intensity for reflected light by turning the Intensity/LM knob (Fig. 4-20/
3
).
•
Finally, sharpen the image of the specimen.