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OPERATION
Axiolab 5
Illumination and contrast methods in reflected light
ZEISS
05/2019
430037-7444-001
113
•
For specimens with medium contrast characteristics, set the aperture diaphragm with the knurled
wheel (Fig. 4-19/
1
) to between 2/3 and 4/5 of the exit pupil diameter of the objective.
In most applications, this aperture diaphragm setting provides optimal contrast at almost ideal resolution,
and is therefore the best compromise for the human eye.
•
Then reinsert the eyepiece, adjust the focus with the coaxial coarse and fine focusing knobs and adjust
the brightness to the reflected light specimen. Readjust aperture stop diameter after each objective
change.
NOTE
Never use the aperture diaphragm for controlling image brightness. Use the Intensity/LM knob
(Fig. 4-19/
4
) for illumination intensity!
1
Knurled wheel of aperture diaphragm A
2
Knurled wheel of luminous-field diaphragm F
3
Nosepiece
4
Intensity/LM knob
5
Focusing drive
6
Reflector turret
Fig. 4-19
Microscope settings in reflected light brightfield microscopy