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YSI 2900 series, Operation And Maintenance Manual

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YSI 2900 series Operation And Maintenance Manual Download Page 142

141 

 

 

Sample Report (Brief) 

======================== 

  Sample Results Report 

======================== 

Batch:       TestBatch-1 
Analyte:         P96_B02 

------------------------ 

3A: Ammonium 

106 mg/L 

Wed 10/04/2015 1:31:09 

======================== 

 
 

======================== 

  Sample Results Report 

======================== 

Batch:       TestBatch-1 
Analyte:         P96_B02 

------------------------ 

3B: Potassium 

193 mg/L 

Wed 10/04/2015 1:31:09 

======================== 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

IB mV

 (Initial Baseline Voltage). The initial baseline voltage is 

monitored before a 

sample or calibration. The IB voltage must be stable and between -40 and 60 mV. 

NPL mV

 (Net Plateau Voltage). This is the peak voltage minus the baseline voltage. 

xNPL nA

 (cross Net Plateau Current). Net plateau current of the other sensor in the module. 

PL Slope 

(Plateau Slope). The slope is reported in mV per minute. An excessive slope can be an indicator of ISE 

problems. 

Avg

 (Average). The average PL voltage for the calibration sequence (last 3 cycles of each calibrator). 

Std Dv

 (Standard Deviation). The standard deviation of the PL voltage for the calibration sequence (last 3 cycles of each 

calibrator). Must be less than 0.2. 

->Coef

 (Selectivity Coefficient). The response of the ISE to one calibrator vs. the other calibrator. Calculated during the 

last 3 cycles of each calibrator. Must be greater than zero and less than 0.3 for ammonium; greater than zero and less 
than 0.1 for potassium. Depending on the application, the 2950 may operate with an ammonium coefficient as high as 
0.40, provided it passes the daily checks in section 5.1. However, it is the user’s responsibility to determine if operation is 
acceptable for their specific application. 

->Slp

 (Slope). The response of the ISE in mV per decade over the range of calibrator solutions. Must be greater than 52 

mV/decade. 

End Point

 is the time from dispensing the sample into the sensor module until the instrument reads the probe signal. The 

value for ISEs is 30 seconds.  

Sample Report (Detail)

 

 

 

 

======================== 

  Sample Results Report 

======================== 

Batch:             P96-1 
Analyte:         P96_B02 
------------------------ 
3A: Ammonium 

105 mg/L 

 IB mV 

20.62 

 NPL mV 

8.23 

 xNPL mV 

1.23 

PL Slope mV/m 

0.23 

Temp (C) 

25.33 

Volume (uL) 

 

25 

Dilution Factor 

x1 

Wed 10/04/2015 1:31:09 
  YSI 2950D – 15J000024 

======================== 

 
 

======================== 

  Sample Results Report 

======================== 

Batch:             P96-1 
Analyte:         P96_B02 
------------------------ 
3B:Potassium 

196 mg/L 

 IB mV 

25.83 

 NPL mV 

13.25  

 xNPL mV 

2.23 

PL Slope mV/m 

0.13 

Temp (C) 

25.33 

Volume (uL) 

 

25 

Dilution Factor 

x1 

Wed 10/04/2015 1:31:09 
  YSI 2950 – 15J000024 

======================== 

 

 

 

Summary of Contents for 2900 series

Page 1: ...YSI 2900 Series Biochemistry Analyzers Operations and Maintenance Manual ...

Page 2: ... 4 3 Connect 2960 Online Monitor 16 4 4 Align Sipper 16 4 5 Configure Instrument Chemistries 19 4 6 4 6 1 Assign Chemistries to Probes 19 4 6 2 Assign Reagents 22 Prepare and Install Buffer Solutions 24 4 7 4 7 1 Prepare Buffer 25 4 7 2 Install Buffer Solution s 25 Install Calibrator Solution s 25 4 8 Install Membranes and ISEs 26 4 9 4 9 1 Enzyme Membranes 26 4 9 2 Ion Selective Electrodes 27 Pri...

Page 3: ...c 50 6 5 4 Filtrate Pump 51 6 5 5 Module 51 Start Monitor 56 6 6 6 6 1 Manual Sample during Monitor Session 57 Stop Monitor 58 6 7 Control Setup 58 6 8 6 8 1 Control Type 58 Print Monitor Configuration 67 6 9 7 System Configuration 68 Settings 68 7 1 7 1 1 System 68 7 1 2 Fluid Detection Bottles 70 7 1 3 Display 71 7 1 4 Auto Cal Settings 73 7 1 5 Scheduler 74 7 1 6 21 CFR Part 11 76 7 1 7 Date Ti...

Page 4: ...erational Checks and Maintenance 119 Cleaning Disinfecting and Decontaminating 119 9 1 9 1 1 Touch Panel 119 9 1 2 Decontamination Procedures 119 Daily Maintenance 120 9 2 9 2 1 Empty the Waste Bottle s 120 9 2 2 Check the Calibrator Bottle s 120 9 2 3 Check the Buffer Bottle s 120 9 2 4 Check for Leaks 120 9 2 5 Clean up Spills 120 9 2 6 Daily Operational Checks 120 Monthly Maintenance 120 9 3 9 ...

Page 5: ...ement Methodology 150 12 3 Baseline Stability 151 12 4 Calibration 151 12 5 Linearity 151 12 6 Temperature Compensation 152 12 7 Level Sensing 152 12 8 13 Warranty and Repair 152 Limitation of Warranty 152 13 1 13 1 1 Shipping Instructions 152 13 1 2 Cleaning Instructions 153 YSI Factory Authorized Service Centers 154 13 2 14 Notices 155 Declaration of Conformity 155 14 1 Declaration of Conformity...

Page 6: ... Life Sciences web site at www ysi com lifesciences User Features 1 2 Slim modular design Easily expand analytes or chemistries Multiple units use much less bench space Proprietary enzyme electrode Fast accurate and analyte specific results Uses biological separation technology No hazardous chromatography solvents to dispose of Icon driven user interface with touchscreen Easy to learn Data downloa...

Page 7: ...trix Output signals Serial USB and RS232 Power requirement 100 240 VAC 10 50 60 Hz 5 42 Watts nominal Working environment Ambient temperature 15 35 C Relative humidity 10 75 non condensing Regulatory compliance ETL CE RoHS 61010 1 compliance Pollution degree 2 Installation category 2 Altitude 2000m Atmosphere 75 KPa to 106 KPa Indoor use only Instrument dimensions 14 0 wide x 20 5 deep x 15 75 hig...

Page 8: ...cle 2 Use ONLY fuses of the type supplied Replacement power cords and fuses can be obtained from YSI or your Dealer Representative 3 Do NOT use an extension cord without protective grounding 4 Do NOT remove rear cover There are no user serviceable parts inside 5 Repairs are to be performed only by trained and approved personnel 6 This instrument must be connected to a protectively grounded earthed...

Page 9: ...d or damage to equipment Attention consulter la documentation jointe Cette mise en garde indique qu une mauvaise utilisation de l instrument peut entraîner une blessure légère ou grave chez une personne et ou un endommagement du matériel Biological Risks Risques biologiques Chemical Irritant Irritant chimique Manufacturer Fabricant Authorized Representative in the European Union Représentant agréé...

Page 10: ...our authorized dealers sold the system to you call YSI Life Sciences Customer Service at 800 659 8895 or 937 767 7241 and we ll be happy to help you 1 After removing the instrument from the shipping box tilt the display to the full upright position Figure 3 1 2 Grasp the hand hold in the right side cover of the instrument and pull up and out to remove the cover NOTE Leave the cover off the instrum...

Page 11: ...ng the 2900 Series The following list shows the basic items required 2900 Series Instrument with AC Power Cord Bottle Racks with Reagent Level Sensing YSI 2936 2938 or Bottles without Reagent Level Sensing YSI 2934 2937 YSI Buffer s YSI Calibrator Standard s YSI Linearity Standard s YSI Membrane s YSI ISE probes 2950D 3 and 2950D 4 models only YSI 2960 Online Monitor optional YSI 2901 Printer opti...

Page 12: ...ion 1 Plate and rack holder accepts most standard plates racks for batch sampling of up to 96 samples Station 2 Test tube holder for manual sampling Buffer Pumps not shown Draw buffer from the buffer bottles pump it through the Valves Sipper Pump and the Sipper and flush the Sample Module Calibrator Pumps not shown Draw the appropriate standard solution from the Calibrator Bottles and fill the Cal...

Page 13: ... full Rear Panel Figure 3 5 Power Switch The main power switch is an on off rocker switch 0 off and I on located on the back of the instrument Fuse Holder The fuse holder houses the power line fuse and opens up for fuse replacement Power Receptacle One end of the power cord supplied plugs into this receptacle while the other end plugs into a properly grounded electrical outlet The instrument will ...

Page 14: ...e large diameter waste tube into the hole in waste bottle 1 b Connect one end of a short cable marked to the threaded fitting on the waste bottle 1 cap and connect the other end to the Waste 1 top fitting on the instrument c Connect the tubing marked B1 and one end of a short cable to the fittings on the buffer bottle 1 cap and connect the other end of the cable to the Buffer 1 2nd fitting on the ...

Page 15: ...ble to the B2 2 nd from top on left fitting on the instrument e Connect the tubing marked B3 and one end of a long cable to the fittings on the buffer 3 bottle cap and connect the other end of the cable to the B3 2 nd from top on right fitting on the instrument f Connect the tubing marked C2A and one end of a short cable to the fittings on the calibrator 2A bottle cap and connect the other end of ...

Page 16: ... or 20 Amp circuit breaker The instrument will automatically adjust the voltage as needed If you are located outside the United States see Appendix for Line Power Cord and Plug Wiring WARNING Keep your hands clear of the sipper while the instrument is in operation 2 Turn the instrument on with the main power switch on the rear panel After about 30 seconds the Initializing window should appear 3 Th...

Page 17: ...tely adjusted WARNING Keep your hands clear of the sipper while the instrument is in operation 1 From the Sipper tab of the Service screen touch Module 1 2 The Select Location screen will appear Select Module 1 The sipper will move to sample module 1 and should be centered above the cone shaped opening in the top of the module If the sipper does not move make sure the packing material was removed ...

Page 18: ...red then touch at the bottom right of the adjustment window Sipper Adjustment Position Figure 4 3 5 Touch to save the position and close the confirmation window 6 To test the alignment of the sipper Touch Inject to lower the sipper then touch Retract to raise the sipper back up If necessary touch Position and repeat the adjustment ...

Page 19: ...r should be right at the top of the module Use the arrow buttons to lower or raise the sipper until the tip of the sipper is even with the top of the module 9 Then touch at the bottom right of the adjustment window 10 Touch to save the depth and close the confirmation window 11 Check the sipper alignment at the Cal 1A Cal 1B and Drain 1 locations and adjust the position if necessary ...

Page 20: ... your 2950 analyzer After you have aligned the sipper with all installed modules touch the Interlock button and change it back to On to enable the safety interlocks Configure Instrument Chemistries 4 6 Before operating the 2900 Series you must set the instrument parameters 4 6 1 Assign Chemistries to Probes 1 From the main display touch 2 From the Module 1 tab touch the Probe A membrane button 3 S...

Page 21: ...eagents should be installed 5 To run a second chemistry in module 1 touch the Probe B membrane button Only chemistries that can be run simultaneously with your selected chemistry will be displayed 6 Select the chemistry you want to measure The Probe B Membrane button will now show the chemistry you have selected ...

Page 22: ... chemistries for the first module proceed to the next installed module on your instrument and repeat the procedure NOTE Changing chemistry assignments will change the calibrator values back to the default settings If your instrument has an ISE module 1 From the Module 3 tab touch the Probe A membrane button and select Ammonium 2 The Probe A Membrane button will now show the chemistry you have sele...

Page 23: ...rrow on the right of the screen The Assign Reagents screen will be displayed Reagents needed are listed on the left of the screen Bottle positions are listed on the right Each reagent must be assigned to a bottle position 4 6 2 1 Buffers 1 Touch the B1 button to display the Select Buffer screen Next Arrow ...

Page 24: ...be assigned to the B1 button Note that the selected buffer is now marked out in the list of reagents needed 3 For additional buffer assignment please repeat process 4 6 2 2 Calibrators 1 Touch the Cal 1A button The Select Calibrator screen appears ...

Page 25: ...arked out Prepare and Install Buffer Solutions 4 7 Caution To prevent possible damage due to an electrostatic discharge do NOT touch the metal tips of the connectors located at the ends of the bottle leads Handle only the insulated section of the connectors Prepare the system buffers and fill the buffer bottles as indicated on the display ...

Page 26: ...e with enough reagent water distilled or deionized to make 1000 mL 4 7 2 Install Buffer Solution s Unscrew and remove the lid from one of the buffer bottles IMPORTANT When adding fresh buffer to the Buffer Supply Bottles make every effort to avoid contamination of the lid and level sensor assemblies Pour the prepared buffer into the buffer bottle Install the bottle in the rack as indicated on the ...

Page 27: ...process for any additional calibrator bottles Cal1A Cal3B as indicated Install Membranes and ISEs 4 9 4 9 1 Enzyme Membranes Each biosensor probe installed in your instrument is fitted with a protective shipping membrane which must be removed and replaced with a new membrane Make sure you install the correct membrane for each chemistry you are measuring Enzyme membranes are color coded for each ty...

Page 28: ...4 5 9 Wipe excess salt solution from the probe body 10 Install a stir bar in the module 11 Then return the enzyme probe to the module 12 Finger tighten the probe retainer so that the O ring seals the probe in place Do not overtighten 13 Return the membrane holder to the foil pouch and refrigerate it 14 Note the expiration date on the membrane package 15 Repeat this procedure for the remaining enzy...

Page 29: ... retainer into the module finger tighten only do not overtighten Figure 4 9 6 Install a stir bar in the module 7 Secure the ammonium and potassium ISEs in place by screwing the probe retainers into the module finger tighten only do not overtighten 8 Connect the ISE cables to the matching connectors located at the rear of the instrument O ring Retainer Potassium ISE Reference Electrode Ammonium ISE...

Page 30: ...rom the Service screen touch the Pumps tab 2 Touch the button under B1 Pump to turn it on 3 The instrument will prime the B1 buffer solution 4 Once buffer flows from the end of the sipper touch the button under B1 Pump to stop the pump 5 Repeat this procedure to prime all other buffer bottles and calibrator bottles you have installed Prime all installed calibrator bottles daily to remove air bubbl...

Page 31: ... the instrument will purge buffer to waste if the incorrect buffer is in the line 2 Observe the ISE voltage values They should be between 40 and 60mV and stable 3 Check the module it should be full of buffer 4 If necessary touch the Flush button to flush the sample module again Please note that when the instrument is first powered up it may take an hour for the voltages to stabilize Once biosensor...

Page 32: ...ell plate 2 Press the Run icon to process it as a sample 3 From the Run Batch tab touch New 4 Choose from the selection of supported racks and plates 5 We highly recommend renaming the rack plate Daily Checks by touching its ID to indicate that it contains your daily check batches 6 After selecting your plate rack touch the Edit button 7 Touch the location of each sample for the first batch Select...

Page 33: ... only the chemistries that require the FCN test 10 To change the Batch Name from the default value of TestBatch touch the TestBatch button The keypad window will appear 11 Type your new batch name and touch DONE 12 Touch to save the batch ...

Page 34: ...ry that corresponds to the linearity standard in that sample location 16 Touch to save the batch 17 Create additional batches for each linearity solution 18 Touch Close when all batches are created 19 Load the plate rack in the sampling station 1 20 Touch Start to run the FCN and the linearity standards as samples The analyzer will calibrate as required and run the batches 5 1 3 Results 21 Touch t...

Page 35: ...oubleshooting Section Chemistry Membrane Calibration Standard FCN Limit 2 Choline 2771 2772 0 02 g L Ethanol 2786 2790 2 00 g L 0 05 g L Ethanol HC 7150 7151 25 0 g L 0 10 g L Glucose 2365 2776 or 2747 0 05 g L L Glutamate 2754 2755 0 06 g L L Glutamine 2735 2736 0 06 g L L Lactate 2329 2776 0 03 g L Methanol 2725 2726 1 00 g L 0 05 g L Sucrose 2703 2780 0 10 g L Xylose 2761 2767 0 05 g L Table 6 ...

Page 36: ... 0 50 g L 1530 2 67 g L 2 54 to 2 80 Lactose 2783 5 00 g L 2784 25 00 g L 23 75 to 26 25 Methanol 2726 1 00 g L 2726 2 50 g L 2 38 to 2 63 Potassium 3 2971 1 00 g L 7179 0 20 g L 0 190 to 0 210 Sucrose 2780 5 00 g L 2778 25 00 g L 23 75 to 26 25 Xylose 2767 20 g L 2768 30 g L 25 5 to 34 5 Table 6 2 Sample Preparation 5 2 A variety of sample types can be analyzed with the 2900 Series Generally the ...

Page 37: ...on Factor Units Multi Sample multiples of each sample location in this batch or Repeats multiples of the entire batch Please note that the instrument does not automatically dilute samples 9 If you diluted your samples a Touch the Dilution Factor 1 button b Enter your dilution factor then touch OK 10 To change the number of sample replicates a Touch the Multi Sample 1 button b Enter the number of t...

Page 38: ... 17 Previously exported plates can be imported later using the Import button 5 3 2 Load Samples 5 3 2 1 R24 and P6 P96 Racks Plates 1 Open the front door of the instrument 2 Insert the plate rack end marked A1 first into the instrument Slide the front edge of the plate rack in until it stops Figure 5 1 3 Gently lower the rear of the plate rack and push it down into position ...

Page 39: ... 2 R4 or R8 Tube Racks 1 Open the front door of the instrument 2 Insert the R4 or R8 tube rack into the cavity just inside the front door Figure 5 3 3 Insert your sample tubes into the tube rack you installed R8 Tube Rack ...

Page 40: ...a module to view its status 3 The virtual printer window displays details of previous samples and calibrations 4 Use the arrow buttons to scroll the printer window Run Stat 5 4 A Stat sample at Station 2 runs without stopping a plate analysis or monitor session that is in progress NOTE The Sipper is not designed to pierce septa Select module to view status Virtual Printer ...

Page 41: ... spring clip b Slide it up all the way until it rests below the notch at the top The test tube holder accepts tubes sizes up to 16x100mm Any container other than this should be sampled manually by holding the sample at Station 2 Figure 5 5 3 Touch Configure to setup the Stat sample 4 Select the chemistries and units for the sample ...

Page 42: ... screen 6 Touch to run the Stat sample at Station 2 If a Station 1 batch is in progress the Stat sample will run as soon as the required module s is available 7 The Stat sample results are displayed on the Run Stat tab Stat sample results ...

Page 43: ...ent control systems The 2960 may draw sample from a process stream bioreactor or other suitable source and deliver sample to the analyzer The system can operate unattended for days or weeks provided sufficient reagent supply is considered The sample volume required for each analysis varies somewhat depending on the distance flow rate and fluid interface used however typically 1 5 milliliters is su...

Page 44: ...n addition the user has the ability to adjust full scale analog output for each chemistry The 2960 also provides three discrete signal outputs TTL logic level to control external pumps which can be used to replenish nutrients or optimize byproduct concentrations 2960 Installation 6 1 1 Connect the small end of the supplied USB cable to the mini USB socket on the back of the 2960 2 Connect the larg...

Page 45: ...upply to the power socket on the back of the 2960 5 Using a 3 32 Allen hex wrench remove the screw from the right rear of the 2900 Series deck as shown below Discard the screw Figure 6 3 6 Using a 9 64 Allen hex wrench and the long hex screw provided with the 2960 install the Monitor Sample Cup on the 2900 Series deck see figure below Power Connection Mini USB Connection Analog Control Connection ...

Page 46: ... larger diameter waste tubing from the fitting on the left side of the 2960 Online Monitor to the waste fitting on the side of the Monitor Sample Cup inside the 2900 Series 9 Run the larger diameter waste tubing from the fitting on the right side of the 2960 Online Monitor to an appropriate waste container Monitor Sample Cup To a waste container To bottom of Monitor Cup To side of Monitor Cup ...

Page 47: ... of the adjustment window to save the depth setting Sample Interface 6 3 When configured appropriately the 2960 automatically samples a bioreactor process stream or other suitable sample source Since color turbidity optical density and many other physical factors do not affect the YSI enzyme biosensor filtration and or dilution may not be necessary and the 2960 may draw the sample directly If cell...

Page 48: ...nto the 2960 see Section 9 6 2960 Maintenance for details The antiseptic cycle must be enabled and the antiseptic solution must be primed immediately after the tubing is reconnected Electrical Interface 6 4 6 4 1 Analog Outputs The 2960 interface provides additional signals to aid in synchronizing the reading of the analog outputs In addition to the two analog outputs three logical signals are pro...

Page 49: ... each i e whether 5 volts turns on or off the external device is selectable See 6 5 Monitor Setup 6 4 3 Auxiliary Connector Signal List On the back of the 2960 is the 15 pin D type connector where the signals emanate The following table relates the signals with the connector pin positions and cable wire colors 2960 Signal Pin Wire color Ground 1 Black White Ground 2 Orange Black Ground 3 Blue Blac...

Page 50: ...the first time touch the Edit button to edit the default monitor configuration To add additional configurations in the future touch New to create a new configuration 6 5 1 Name 4 Touch the Name button and enter a name for this configuration ...

Page 51: ... to purge the line from the sample source to the solenoid valve The volume of the supplied sample tubing is 5 1uL per inch Flow Rate uL min Purge Time Seconds Flow Rate uL min Purge Time Seconds 100 900 1400 65 200 450 1500 60 300 300 1600 57 400 225 1700 53 500 180 1800 50 600 150 1900 48 700 129 2000 45 800 113 2100 43 900 101 2200 41 1000 90 2300 39 1100 82 2400 38 1200 75 2500 36 1300 69 Table...

Page 52: ...rge pump of the 2960 This option is normally not used since the 2960 pump can transport most samples to the Monitor sample cup If an external filtrate pump is used the flow rate must be maintained at or above 580uL minute in order to guarantee that the sipper does not aspirate air during the sampling process The default filtrate pump output is Active High 5V Touch the Filtrate Pump Active High but...

Page 53: ...onitor sample when an autocalibration will be initiated When in the monitoring mode and when the 2900 Series is stabilized in terms of calibration drift you may elect to disable autocalibrations related to time and number of samples and use the PreCal option only 6 5 5 2 Interval 12 Touch the Interval 00 30 00 button and enter the time interval between your monitor samples for Module 1 6 5 5 3 Che...

Page 54: ... 6 5 5 4 Analog Output 16 Touch the DAC None button to enable the analog output for this probe 17 Select the Analog output for this probe 18 The Analog output can be set to 5 Volts or 10 Volts full scale Touch the DAC 5V button to change the Voltage to 10V 19 Touch the 10 00g L button to set the sample value that corresponds to the full scale Voltage ...

Page 55: ... Probe A changes and return to the Edit Chamber 1 screen 22 To monitor an additional chemistry touch the Probe B Edit button 23 Enter the Chemistry and Units for Probe B Analog output and Control can also be set for Probe B 24 Touch Save to save your Probe B changes and return to the Edit Chamber 1 screen Select Probe B ...

Page 56: ...hamber 1 changes and return to the Edit Configuration screen 26 Touch Save to save your Configuration and return to the Select Configuration screen 27 Touch Select to choose the selected configuration and return to the Monitor screen ...

Page 57: ...nd selected your monitor session touch 2 Enter a name for this specific run then touch DONE 3 The monitor session will start The instrument will calibrate the required probe s if necessary or if Precal is enabled then run the first monitor sample ...

Page 58: ... Sample during Monitor Session A plate rack or Stat sample may be run during a monitor session provided there is sufficient time between monitor samples If you want to manually initiate a monitor sample without waiting for the Next Sample Time to arrive 1 Touch the Start Manual Sample button 2 Select the Chamber that you want to start Sample Results Zoom buttons Reset Units ...

Page 59: ...to accommodate all circumstances in a single form of the PID control algorithm Certain assumptions apply to the application of this control scheme of which the user should be aware Deviations from these assumed conditions will result in degraded regulation Assumptions 1 Regulation is begun enabled when the analyte concentration is within 10 of the set point 2 The sampling interval is regular That ...

Page 60: ...59 2 Touch New to create a new monitor configuration or select an existing configuration and touch Edit 3 Touch the Module 1 Edit button 4 Touch the Edit button for the probe you want to control ...

Page 61: ...r this channel PID or Threshold and follow the corresponding instructions in the section below 6 8 1 1 PID For proportional control select PID as the Control Type Setpoint Touch the Setpoint 10 00g L button and enter the concentration at which the analyte will be regulated ...

Page 62: ... via feed stock additions Auto Activation Auto Activation automatically starts control once the sample value is within a certain percentage of the setpoint to minimize over under correction Using the default value control will not start until the monitored sample value is within 5 of the setpoint To change this value touch the Auto Activation 5 00 button and enter the new value When using Auto Act...

Page 63: ... feed pump Touch the Maximum Rate of Change 10 00g L min button and enter the maximum rate of change expected during the monitor session maximum rate of consumption of the measured analyte during the run Touch the Feed Concentration 0 00g L button and enter the concentration of your feedstock solution The minimum value required is displayed in the Min Feed Stock box ...

Page 64: ... to enter the TPU manually Manual allows you to enter the TPU value that you calculated yourself Touch the TPU 10 00 sec g L button and enter your manually calculated TPU value Touch Save to save changes and return to the Edit Probe screen ...

Page 65: ...utput line that your external feed pump is connected to The default feed pump output is Active High 5V Touch the Feed Pump Line High button to change it to Low if your feed pump requires an Active Low signal 0V to turn on Touch Save to save changes and return to the Edit Chamber 1 screen ...

Page 66: ...nfiguration screen Touch Save to save changes and return to the Select Configuration screen Touch Select to choose the selected Configuration and return to the Monitor screen 6 8 1 2 Threshold For simple threshold alarm select Threshold as the Control Type ...

Page 67: ...d Pump Line None button and select the 2960 output line that your external alarm pump is connected to The default output is Active High 5V Touch the Feed Pump Line High button to change it to Low if your alarm pump requires an Active Low signal 0V to turn on Touch Save to save changes and return to the Edit Chamber 1 screen Touch Save to save changes and return to the Edit Configuration screen ...

Page 68: ...e reviewed on the YSI 2901 Printer or from the Status tab SITINI ONLINE SAMPLER CONFIGURATION REPORT Config Name GLUCOSE CON TROL Antiseptic Cycle 00 00 30 Purge Time 00 01 00 Flow Rate 2000 uL min Filtrate Pump Active Level High Chamber 1 PreCal 00 05 00 Sample Interval 00 30 00 Probe 1A Chemistry Glucose Unit g L DAC None Control Type PID Setpoint 1 00 g L Auto Activation 5 TPU 48 00 Error Direc...

Page 69: ...nly an Administrator can access Settings 7 1 1 System Touch the System tab if not already selected The System tab is used to select the type of sample and calibration reports enable sipper fluid detection and print the configuration 7 1 1 1 Report Details To change the Sample or Calibration Reports touch the button below it to cycle through the selections Detailed Brief or None Touch Detailed ...

Page 70: ... to enable the remote host to send commands to the 2900 Series 7 1 1 3 Print Configuration Touch the Print Configuration button to send the current instrument configuration to the printer 7 1 1 4 Sipper options Touch the Sensitivity High button and change it to Low for samples with high conductivity Touch the Fluid Detector On button and change it to Off to completely disable sipper fluid detectio...

Page 71: ...e stations Touch the When no fluid detected button and select the sample fluid detection mode you prefer Error Produce an error when no sample is detected no sample result Warn Use fixed depth when no sample is detected report a sample result 7 1 2 Fluid Detection Bottles From the Settings screen touch the Fluid Detection tab ...

Page 72: ...are off check bottle fluid levels regularly to prevent waste fluid from backing up into sample modules 7 1 3 Display From the Global Settings screen touch the Display tab Adjust the display brightness or sound volume by touching or 7 1 3 1 Touch Screen Calibration The touch screen is calibrated at the factory and should not require user calibration Touch the Calibrate Display button to enter touch...

Page 73: ... After you have held the in all four corners the touch panel is calibrated Touch screen calibration can also be initiated by touching anywhere on the Initialization screen while the instrument is powering up 7 1 3 2 Clock Display Format Touch the Clock display format button to select 12 hour or 24 hour clock format OK OK ...

Page 74: ...YSI s stated specifications are based on the default settings These selections are provided as part of the overall concept of the 2900 Series flexibility To change the value of a Calibration parameter simply touch the value to open the numeric keypad Enter the new value then touch OK NOTE To disable a Calibration parameter touch the ON button and change it to OFF With AutoCal set to On the instrum...

Page 75: ... for that module Touch the Interval hours button and enter the number of hours between Auto flushes Touch the Enabled ON button and change it to OFF to disable Auto flush for this module Touch Save to save your changes 7 1 5 Scheduler From the Settings screen touch the Scheduler tab ...

Page 76: ... the Scheduled calibration OFF button and change it to ON Touch any days to select them Days of the week that the scheduler is enabled are green Touch the Hour button 9 Enter the Hour each day that the instrument should calibrate in 24 hour format 0 23 then touch OK Touch the Minute button 00 and enter the Minutes Touch OK ...

Page 77: ...making your changes touch the X button to return to the main display 7 1 6 21 CFR Part 11 FDA 21 CFR Part 11 Electronic Records Electronic Signatures was established by the FDA to define the requirements for submitting documentation in electronic form and the criteria for approved electronic signatures Since YSI 2900 Series analyzers generate electronic records these systems must facilitate compli...

Page 78: ...77 Touch the to enable 21 CFR Part 11 mode Enter a Name User ID and 8 character PIN to create at least one Admin account Note that the PIN must be exactly 8 characters Touch Save ...

Page 79: ...ve the Admin box blank if you are creating a standard user Touch the Admin box to mark it ONLY if you are creating a new Administrator Note that standard users cannot access the Settings menu Configure menu the Sipper or Stirbar tabs under the Service menu or the Software tab under the Help menu as shown below ...

Page 80: ...tive or Admin fields as required To disable a user remove the X from the Active box After you have finished making your changes touch Save Touch the X button to return to the 21 CFR Part 11 screen 7 1 6 2 View Audit Trail and Event Log Audit Trail Touch the View History button to display the audit trail ...

Page 81: ...n the log Export Plug a flash drive into the 2900 Series USB port then touch the Export button to send the Audit Trail and Event Log to the flash drive A folder named YSI BiochemistryAnalyzer 21 CFR Part 11 followed by the Machine ID will be created on the flash drive When you have finished exporting touch the X button to return to the 21 CFR Part 11 screen ...

Page 82: ...or can change the date time Touch the current date on the calendar to select it Touch the Hour button and enter the current hour in 24 hour format 0 23 then touch OK Touch the Minute button and enter the current minute then touch OK When you have finished entering the date and time touch OK to return to the main display Date Time Hour Date ...

Page 83: ...s used at Station 1 and the calibrator wells NOTE If 21 CFR Part 11 mode is enabled only an Administrator can access the Sipper tab 7 2 1 1 Interlock Touch the Interlock button and change the status to Off to disable the interlock switches on the front door and side panel when aligning the sipper WARNING Keep your hands clear of the sipper while the instrument is in operation Always turn the Inter...

Page 84: ...pper at other locations touch the button under Location Touch the button for your location Station 2 for example Touch the Position button and use the arrow buttons to align the sipper with the selected location Location ...

Page 85: ...pper back up If necessary touch Position and repeat the adjustment 7 2 1 2 Depth Touch Depth and select the location The sipper will move to the selected location Sample Modules use the up and down arrows to set the tip of the sipper even with the top of the Module Sample Locations use the up and down arrows to set the maximum depth the sipper will travel at that sample location ...

Page 86: ...bout half way as it does when it dispenses a sample into the sample module When you have finished testing the sipper pump touch the X button to return to the main display 7 2 2 2 Buffer Pumps Touch the ON button under a buffer pump The selected buffer pump will run Touch the OFF button to stop the pump When you have finished testing the buffer pumps touch the X button to return to the main display...

Page 87: ... flush the sample module again Note that when the instrument is first powered up it may take several hours for the baseline currents to drop below 6 nA Touch the Flush button under Module 3 to flush the sample module with ISE buffer Note that the instrument will purge buffer to waste if the incorrect buffer is in the line Observe the ISE voltage values They should be between 40 and 60mV and stable...

Page 88: ... active probe up to 5 times before aborting calibration If calibration fails see 11 Troubleshooting 7 2 4 Stirbar From the Service menu touch the Stirbar tab NOTE If 21 CFR Part 11 mode is enabled only an Administrator can access the Stirbar tab ...

Page 89: ...o decrease the stir speed until the stir bar is spinning smoothly Touch the Jump button The speed should increase to Jump speed and the stir bar should jump If necessary increase the jump speed until the stir bar is jumping If the stir bar won t jump even with the speed set to maximum 100 replace it Touch the Spin button Verify the stir bar is spinning smoothly and not jumping If necessary reduce ...

Page 90: ...e Opened Valve Antiseptic button to change the state of the valve and change it to Sample Prime Monitor Pump Touch the Pump Sample Off button and change it to On to prime the monitor sample line The pump will turn off automatically If you are using an antiseptic solution touch the Pump Antiseptic Off button and change it to On to prime the antiseptic solution The pump will turn off automatically N...

Page 91: ...e tab Touch the scroll buttons to page through plates Touch a specific plate to highlight it then touch View to display the sample data for the selected plate Sample data for the first sample in the batch A1 is displayed Touch any other sample location to display the sample data Touch the sample result to show details ...

Page 92: ...Filter Touch the Name button and enter a plate name to filter the data Only plates that begin with that name will be displayed Export Check the To Export box for each batch that you would like to export to a flash drive Select to show details Select sample ...

Page 93: ...u Cross Net Plateau Temp Adj Plateau Slope Temperature Errors P96 1 TestBatch 1 12 11 2013 10 31 Complete P96_A04 Glutamine Probe2B 0 10872 g L 30 20 2 27 32 30 2 27 30 03 30 38 30 83 31 12 0 022 24 55 P96 1 TestBatch 1 12 11 2013 10 31 Complete P96_A04 Glutamate Probe2A 1 43929 g L 30 20 0 52 31 36 0 89 30 83 31 12 0 268 24 55 P96 1 TestBatch 1 12 11 2013 10 29 Complete P96_A05 Glucose Probe1A 4 ...

Page 94: ... return to the Data screen Name Filter Touch the Name button and enter a monitor session name to filter the data Only sessions that begin with that name will be displayed Export Check the To Export box for each session that you would like to export to a flash drive ...

Page 95: ...ontain the instrument s Machine ID along with the date and time 7 3 3 Calibration Touch the Calibration tab Plug a flash drive into the 2900 Series USB port Select a date range then press the Export button to send calibration data to the flash drive When you have finished exporting data touch the X button to return to the Main screen A folder named YSI BiochemistryAnalyzer will be created on the f...

Page 96: ...3 10 43 Glucose 2776 2 5 g L 30 25 3 240 14 478 3 275 11 239 NaN 0 144 25 20 1B 12 11 2013 10 43 Lactate 2776 0 5 g L 30 25 3 276 14 372 3 303 11 096 NaN 0 172 25 20 2B 12 10 2013 16 11 Glutamine 2736 5 mmol L 30 20 3 758 20 733 3 744 16 976 0 077 0 031 24 86 2A 12 10 2013 16 07 Glutamate 2755 5 mmol L 30 20 4 245 20 151 4 250 15 906 14 238 0 514 24 83 1A 12 9 2013 11 48 Glucose 2776 2 5 g L 30 25...

Page 97: ...lash drive NOTE The configuration file can be imported at a later date to restore the instrument configuration 7 4 2 2 Backup Press Backup to backup the current instrument setup database log files and 21 CFR 11 audit files to the flash drive 7 4 2 3 Restore Press Restore to restore a previous backup file for this analyzer from a flash drive 7 4 2 4 Default To default all instrument settings to the...

Page 98: ...oftware update in the instruments USB port After the Flash Drive Inserted light changes to green touch the Launch Updater button The 2900 Series Updater will be displayed Touch the Update Firmware button to install the new firmware Touch the Update Application button to install the new software When the Installation Complete window is displayed remove flash drive The instrument will restart NOTE U...

Page 99: ... stop the video and return to the Training screen Ethernet Port 7 5 Connect one or more 2900 Series instruments to a LAN or router via the RJ45 Ethernet port 7 5 1 LAN Shared Network Connection Connect one or more 2900 Series instruments to a LAN via the RJ45 Ethernet port 7 5 2 Router Private Network Connection Connect one or more 2900 Series instruments to a router DHCP server via the RJ45 Ether...

Page 100: ...red in a 2900 Series connected to a LAN or router via ftp See 7 4 1 About to view the instrument s current IP address If you are unable to connect via ftp make sure the Passive ftp option is not checked under Advanced Internet Options on your computer ...

Page 101: ...le and calibration data files are stored in the Samples folder of the 2900 Series The file names are BioSample_Machine ID csv BioCalibration_Machine ID csv ISESample_Machine ID csv ISECalibration_Machine ID csv ...

Page 102: ...your reading Methods to do these corrections are described below Once you make the above determinations you can decide whether one of the standard setups described below will be appropriate or whether you will need to customize your setup Sample Volume 8 1 The sample volume range is 10 to 50 µL However this is a nominal volume The instrument does not depend on an accurate absolute value but rather...

Page 103: ...express the sample as a salt of choline you must enter an adjusted calibration value when configuring your instrument measurement parameters For example to express results as a choline hydroxide value program the calibrator value as 204 mg L when using the YSI 2772 Choline Standard calibrator solution Your results will now reflect the concentration of choline hydroxide in the sample Assumptions an...

Page 104: ...Special Considerations The linear range of ethanol is quite limited If you are concerned about linearity monitor the upper range of concentration on a regular basis You may benefit by preparing and using a calibrator with an ethanol concentration close to your sample concentration If you prepare your own ethanol calibrator prepare a solution with ionic strength Level sensing depends on a conductiv...

Page 105: ...uired use reagent water The sample should be lactose free Lactose glycerol and other galactosides such as raffinose and stachyose are substrates for galactose oxidase They may interfere by producing artificially high galactose readings The enzyme membrane integrity test involving the use of YSI 2363 Potassium Ferrocyanide solution is not informative since ferrocyanide exists in the 2705 system buf...

Page 106: ...ation unit conversion is required Special Considerations If sample dilution is required use reagent water If a solution must be prepared from solid glucose use the following diluent and allow about 15 minutes before measuring the sample This is required for glucose which must equilibrate alpha and beta anomers mutarotational equilibrium If your reading is lower than expected you may need to wait s...

Page 107: ... use reagent water If you want to measure MSG monosodium glutamate monohydrate and express the results as mmol L of MSG the calibration parameters listed above are all appropriate If you want to express results as a w v measurement such as mg L MSG then correction for the sodium and water components is necessary To express results as an MSG value change the glutamate units to a w v expression and ...

Page 108: ...int 731 mg L Linearity Check Point 1169 mg L Sample Size 20 µL End Point 30 sec Precision CV n 5 4 or 15 mg L whichever is greater Linearity 4 30 to Cal Point 5 Cal Point to Range Max Typical Working Life 5 days Note See Appendix B Concentration Unit Conversion if concentration unit conversion is required Special Considerations If sample dilution is required use reagent water The sample must be gl...

Page 109: ...e free Lactose and other galactosides such as galactose raffinose and stachyose are substrates for galactose oxidase They may interfere by producing artificially high glycerol readings The enzyme membrane integrity test involving the use of YSI 2363 Potassium Ferrocyanide solution is not informative since ferrocyanide exists in the 2705 system buffer Always use fresh YSI 2705 Buffer in an opaque b...

Page 110: ...brane the enzyme catalase is a concern Catalase destroys hydrogen peroxide Buffer pretreatment may be required If you suspect an electrochemical interference as opposed to an enzymatic interference you may want to use one channel to qualitatively monitor for an interference effect Typical electrochemical interferences include phenols mercaptans hydroxylamine hydrazine and analines If you suspect a...

Page 111: ...ck Point 2 67 g L Sample Size 25 µL End Point 30 sec Precision CV n 10 2 or 0 03 g L whichever is greater Linearity 2 0 05 to Cal Point 5 Cal Point to Range Max Typical Working Life 14 days Note See Appendix B Concentration Unit Conversion if concentration unit conversion is required Special Considerations If sample dilution is required use reagent water D Lactate is not a substrate for L Lactate ...

Page 112: ...or 0 02 g L whichever is greater Linearity 2 0 05 to Cal Point 5 Cal Point to Range Max Typical Working Life 10 days Note See Appendix B Concentration Unit Conversion if concentration unit conversion is required Special Considerations If sample dilution is required use reagent water The sample should be galactose free Galactose glycerol and other galactosides such as raffinose and stachyose are su...

Page 113: ... basis You may benefit by preparing and using a calibrator with a methanol concentration close to your sample concentration If you prepare your own methanol calibrator prepare a solution with ionic strength Level sensing at the Tube Holder depends on a conductive solution A 0 1 K2EDTA solution as a diluent acts as both a conductive solution and a preservative Normal saline solution 0 9 is also an ...

Page 114: ...ection Range 0 1 25 0 g L Calibration Point 5 0 g L Linearity Check Point 25 0 g L Sample Size 25 µL End Point 30 sec Precision CV n 10 2 or 0 02 g L whichever is greater Linearity 2 0 1 to Cal Point 5 Cal Point to Range Max Typical Working Life 10 days Note See Appendix B Concentration Unit Conversion if concentration unit conversion is required Special Considerations If sample dilution is requir...

Page 115: ... Unit Conversion if concentration unit conversion is required Special Considerations If sample dilution is required use reagent water or YSI buffer The sample must be glucose free or at least contain levels low enough not to interfere with the xylose reading since glucose will react with pyranose oxidase See 8 2 17 Simultaneous Glucose and Xylose for xylose specifications when measuring glucose an...

Page 116: ... 5 Typical Working Life 90 days Note See Appendix B Concentration Unit Conversion if concentration unit conversion is required Special Considerations Sodium may be an interferent to the measurement of ammonium with some samples The interference is relatively small on a weight weight basis 1 The 2900 Series does not automatically compensate for the presence of sodium In order to check the potential...

Page 117: ...ow to set up your instrument for simultaneous determination A Probe B Probe Chemistry Glucose L Lactate System Buffer YSI 2357 Calibrator Std YSI 2776 Linearity Std YSI 1531 YSI 1530 Membrane YSI 2365 YSI 2329 Sample Size 25 µL Unit of Conc g L Cal Value 2 50 0 50 End Point 30 sec Note See Appendix B Concentration Unit Conversion if concentration unit conversion is required ...

Page 118: ...ucrose the sucrose precision and linearity are as follows Precision CV n 10 4 or 0 04 g L whichever is greater Linearity 5 Special Considerations When measuring glucose and sucrose the glucose and sucrose sensors must be located in the same module The combined total of glucose sucrose cannot exceed 25 g L The glucose concentration cannot exceed 10 g L if the combined total is near 25 g L since a g...

Page 119: ...t is based on an algorithm that simultaneously measures both xylose and glucose The algorithm utilized takes into account the responsiveness of the xylose membrane to glucose and the slight response of the glucose membrane to xylose Special Considerations When measuring glucose and xylose the glucose and xylose sensors must be located in the same module As with most analytical methods there is a c...

Page 120: ...rom Glutamine reaction Special Considerations The ability to select sample size 10 to 50 µL may help you optimize your system especially if very high or very low concentrations of one or the other of these substrates is a problem The optimal probe current for a calibrator is about 10 nA When measuring glutamate and glutamine the glutamate and glutamine sensors must be located in the same module 9 ...

Page 121: ...d any significant evaporation will cause inaccurate test results Consider changing calibrator daily or more often if required 9 2 3 Check the Buffer Bottle s Replace the buffer if the bottle is low or the buffer has been in the instrument longer than 1 week Clean the buffer bottle and cap with a bleach solution then rinse well with DI water before installing fresh buffer You may find it convenient...

Page 122: ...rom the Service screen Pumps tab touch Off to prime the fresh calibrator through the tubing and cal well After 2 minutes touch On to turn the pump off Repeat this entire procedure for any additional calibrator bottle positions that you use Preventive Maintenance 6 months or 1000 Hours 9 4 Before performing maintenance on the 2900 Series ALWAYS turn the instrument off and unplug the power cord from...

Page 123: ...nt Remove the o rings from the base plate Clean up any salt deposits or fluid on the base plate Be sure that the base plate and all other parts are dry 9 4 3 Enzyme Probe Cleaning With normal use enzyme sensors may become fouled and cease to operate normally A fouled sensor s output current will decrease and calibration may become unstable Since the severity of fouling will vary listed below are m...

Page 124: ... cleaning procedure has been unsuccessfully attempted WARNING The following procedure may cause permanent damage to the enzyme sensor 1 Using a clean new pencil eraser carefully rub the center electrode platinum of the sensor Do not rub the epoxy between the two electrodes or the outer silver electrode 2 To remove any eraser dust or residual rinse sensor with 70 isopropyl alcohol and then reagent ...

Page 125: ...ng connects are not blocked or restricted Replace the seals as shown in Figure 9 5 Extra seals are supplied in the Preventive Maintenance Kit Be sure to reinstall the black spacer between the two small O rings Sipper pump seal replacement Figure 9 5 Reassemble the pump position the plunger as shown in Figure 9 6 and install it back on the instrument Sipper pump plunger position Figure 9 6 SPACER ...

Page 126: ...nnectors from the buffer waste and calibrator bottles on each side of the instrument Lift the bottle tray on the right side of the instrument up and remove it Remove the bottles from the tray s and clean the bottles and caps with the appropriate disinfecting agent see 9 1 Cleaning Disinfecting and Decontaminating Remove the two hex screws holding the pump cover on each side of the instrument Lift ...

Page 127: ...6 Pumps Right side Figure 9 8 Pumps Left side Figure 9 9 Buffer Pump 1 BLUE C1B Pump RED C1A Pump RED Sipper Pump Buffer Pump 2 BLUE Buffer Pump 3 BLUE C2A Pump RED C3B Pump RED C2B Pump RED C3A Pump RED ...

Page 128: ...urn ON the Bottom Valve 4 With the bottom valve ON insert the long section of valve tubing close to the first Y near the clear section into the outer channel of the bottom valve Hold the tubing on both sides of the valve and push it straight into the valve channel until it snaps in 5 From the Service screen Pumps tab touch the ON button under Bottom Valve to turn OFF the Bottom Valve 6 Insert the ...

Page 129: ...ee tubing holders and connect it to the outer channel of the top valve Connect the larger diameter tubing from the top of buffer pumps 2 and 3 to the top fitting of each manifold Connect Buffer bottle tubing marked B2 and B3 to the matching fittings on the opposite side of the manifolds 9 4 7 3 Calibrator Pump Tubing C1A Note that the tubing for C1A is slightly longer than C1B Apply plenty of cali...

Page 130: ...ng marked C2A to the matching fitting on the opposite side of the manifold Figure 9 12 C2B Apply plenty of grease included with the new pump tubing to the shorter section of new calibrator pump tubing Insert the new pump tubing around the pump roller assembly and into calibrator pump C2B Make sure the short section of tubing is on the left side of the pump The tubing attached this fitting connects...

Page 131: ...side of the pump The tubing attached this fitting connects to the right fitting on waste module 3 Place a red pump head cover onto the cal pump and press until it snaps into place Connect the tubing from the right side of the pump to the bottom fitting of the bottom manifold Connect Cal bottle tubing marked C3B to the matching fitting on the opposite side of the manifold 9 4 8 Install Waste Module...

Page 132: ...lacement Caution Touch a bare metal chassis screw before handling the sipper tube to prevent possible damage due to an electrostatic discharge The Sipper can be damaged if it is not properly aligned or if its alignment is disturbed Inspect the Sipper for straightness and condition of the Teflon jacket separating the two stainless steel tubes If the Teflon jacket is torn replacement of the Sipper i...

Page 133: ...sing the hex screw do not over tighten Route the new sipper tubing behind the sipper assembly and connect the other end to the front connector of the sipper pump 9 4 12 Calibrate Sipper Align the Sipper with the sample modules as described in 4 5 Align Sipper 9 4 13 Install Bottles Temporarily place the bottle trays next to the instrument and install the bottles Connect the tubing and cables to th...

Page 134: ...dent The recommended replacement is 350 hours for the silicone waste tubing and 3 months for all other tubings including the PharMed pump tubing solenoid valve tubing antiseptic tubing and sample source tubing The 2990 Preventive Maintenance Kit provides a complete set of tubings for the 2960 If you elect to clean tubings solutions such as 70 isopropanol 0 5 hypochlorite and mild detergents in wat...

Page 135: ...bing first thread the waste line tubing through one chamber of the pump body Install the roller assembly slot in center shaft oriented up into the pump body chamber It will help to twist the roller assembly as you install it to more easily capture the tubing in between the rollers and chamber wall Once in place install the retainer and adjust the lengths of tubing on each side locating the retaine...

Page 136: ...re should be followed when installing the solenoid tubing to ensure correct valve operation and prevent premature tubing failure 1 From the Service menu Monitor tab touch the Opened Valve Antiseptic button and change it to Sample to energize the solenoid 2 Install the Sample Tubing in the outer solenoid valve slot Make sure that the tubing lies completely at the bottom of the slot Do not stretch t...

Page 137: ... water through the system thoroughly empty the bottles reinstall them in the instrument and prime the system with air After all the fluid has been pumped from the system drain the sample modules by temporarily removing one probe Reinstall the enzyme probes and store the instrument with the membranes in place Store the instrument in an environment from 15 35 C 10 75 humidity non condensing Enzyme M...

Page 138: ...s salt build ups air bubbles in the sample module loose connections noisy components etc Review Section 7 2 to learn more about how you can test individual components of the 2900 Series Use the troubleshooting chart in this section to assist you in identifying the problem then use the chart to guide you to a corrective action If the problem cannot be resolved contact YSI Technical Support When com...

Page 139: ... Batch Test Batch 1 Cal Shift 1A Glucose 2 50 g L Analyte P96_A01 IB nA 1 79 NPL nA 17 63 1A Glucose 4 82 g L FB nA 1 51 IB nA 2 11 PL Slope nA m 0 42 NPL nA 32 65 IB Shift 0 65 PL Slope nA m 0 69 NPL Shift 3 64 Temp C 25 9 Temp C 25 9 Volume uL 25 1B Lactate 0 50 g L Dilution Factor x1 IB nA 0 88 Fri 9 9 2015 10 59 37 NPL nA 9 81 YSI 2950 15F000025 FB nA 0 76 PL Slope nA m 0 42 IB Shift 1 95 NPL ...

Page 140: ...an be an indicator of the presence of an interfering substance The message Final baseline error is printed when the instrument cannot adequately flush the sample module PL Slope Slope of the plateau The slope is reported in nanoamps per minute A newly installed membrane may have an elevated plateau slope An excessive slope can be an indicator of the presence of an interfering substance End Point i...

Page 141: ... 0 17 End Point 30 sec Sample size 25 uL Temperature 21 50 C Probe3B 1000 mg L K 2971 NH4 K IB mV 28 40 26 98 NPL mV 6 97 37 42 IB mV 28 50 26 97 NPL mV 6 31 37 45 IB mV 28 63 27 02 NPL mV 6 09 37 42 Avg 6 12 37 43 Std Dv 0 17 0 02 End Point 30 sec Sample size 25 uL Temperature 21 50 C Coef 0 19 0 02 Slp 57 18 60 05 Tue 10 25 2015 11 08 32 YSI 2950 13J000024 Calibration Report Probe3A 500 mg L NH4...

Page 142: ...calibrator vs the other calibrator Calculated during the last 3 cycles of each calibrator Must be greater than zero and less than 0 3 for ammonium greater than zero and less than 0 1 for potassium Depending on the application the 2950 may operate with an ammonium coefficient as high as 0 40 provided it passes the daily checks in section 5 1 However it is the user s responsibility to determine if o...

Page 143: ...librations are repeated until the standard deviation of both sensors is less than 0 20 or the Change Ratio is less than or equal to 1 2 for each calibration solution 3B 1 33 38 3A 1 04 11 3B 2 34 45 3A 2 05 13 3B 3 34 60 0 09 0 44 3A 3 05 08 0 99 0 71 3B 4 34 70 0 18 3 04 3A 4 05 04 0 12 0 52 3A 1 22 91 3B 1 01 45 3A 2 21 91 3V 2 01 96 3A 3 21 94 0 02 1 6 3B 3 01 35 0 26 1 8 3A 4 22 85 0 05 4 7 3B...

Page 144: ...ter probe diagnostics and check probe currents SECTION 7 2 3 Modules POSSIBLE CAUSE Power disruption ACTION Enter probe diagnostics and check probe currents SECTION 7 2 3 Modules POSSIBLE CAUSE Failing enzyme membrane ACTION Perform daily operational checks and replace membrane if necessary SECTION 5 1 Perform Daily Operational Checks 4 9 Install Membranes and ISEs POSSIBLE CAUSE Enzyme Probe surf...

Page 145: ...nA ACTION Dilute sample or adjust sample size down and repeat SECTION 8 1 Sample Volume SYMPTOM MEASUREMENT ERROR PL Slope POSSIBLE CAUSE Stir speed too fast or too slow ACTION Adjust stir speed SECTION 7 2 4 Stirbar POSSIBLE CAUSE Newly installed enzyme Membrane ACTION Enter probe diagnostics and check probe currents SECTION 7 2 3 Modules POSSIBLE CAUSE Newly installed probe ACTION Enter probe di...

Page 146: ...CTION 7 2 3 Modules POSSIBLE CAUSE Newly installed probe ACTION Enter probe diagnostics and check probe currents SECTION 7 2 3 Modules POSSIBLE CAUSE Calibrator solution out of spec contaminated or installed for more than 30 days ACTION Install new calibrator SECTION 4 8 Install Calibrator Solution s POSSIBLE CAUSE Net calibration current PL current below 5 nA ACTION Replace enzyme Membrane and ch...

Page 147: ...ssignment incorrect ACTION Make correct assignment SECTION 4 6 1 Assign Chemistries to Probes POSSIBLE CAUSE Calibrator bottle assignment incorrect ACTION Make correct assignment SECTION 4 6 2 Assign Reagents POSSIBLE CAUSE Damaged or old Membrane ACTION Replace membrane SECTION 4 9 Install Membranes POSSIBLE CAUSE Contaminated or old calibration or linearity standard ACTION Repeat test with new s...

Page 148: ...connected to the Monitor Cup inlet port while the pump is running If fluid flows the obstruction is in the cup or the waste line If fluid does not flow try massaging the tubing to try relieving the blockage If unsuccessful begin disconnecting tubing and forcing air or fluid through with a syringe until you locate the blockage SYMPTOM Bubbles appear at the top of the Monitor Cup when the Monitor Pu...

Page 149: ...eptic instead of sample sample results read approximately zero POSSIBLE CAUSE Tubing routed incorrectly in 2960 solenoid valve ACTION Check 2960 valve tubing installation SECTION 9 6 1Tubing Replacement Solenoid Valve Tubing Replacement ...

Page 150: ...dized at the platinum anode producing electrons Reaction 2 A dynamic equilibrium is achieved when the rate of H2O2 production and the rate at which H2O2 leaves the immobilized enzyme layer are constant and is indicated by a steady state response Figure 12 2 The electron flow is linearly proportional to the steady state H2O2 concentration and therefore to the concentration of the substrate REACTION...

Page 151: ...sure the ammonium ion concentration NH4 or the potassium ion concentration K in solution However as is the case with most ISEs other ions similar in shape size or charge may also pass through the membrane and interfere with the measurement In the case of ammonium and potassium an ammonium sensor will respond to ammonium but will also respond to a lesser extent to potassium Likewise a potassium sen...

Page 152: ...alibration parameters can be altered to tighten or loosen calibration specifications A manual calibration can be initiated from the Run Calibrate tab A STABLE CALIBRATION IS IMPORTANT The instrument re establishes a calibration reference point after every calibration If a difference of more than 2 between the present and previous net calibration values occurs the instrument repeats calibration The...

Page 153: ...ree of charge any product that YSI determines to be covered by this warranty Limitation of Warranty 13 1 This Warranty does not apply to any YSI product damage or failure caused by i failure to install operate or use the product in accordance with YSI s written instructions ii abuse or misuse of the product iii failure to maintain the product in accordance with YSI s written instructions or standa...

Page 154: ... other part is returned or presented for service without a Cleaning Certificate and if in our opinion it represents a potential biological or radioactive hazard our service personnel reserve the right to withhold service until appropriate cleaning decontamination and certification have been completed We will contact the sender for instructions as to the disposition of the equipment Disposition cos...

Page 155: ...8494 Fax 707 307 7130 RJM Sales 454 Park Avenue Scotch Plains NJ 07076 Phone 800 752 9055 Fax 908 322 2160 Giangarlo Scientific 162 Steuben St Pittsburgh PA 15220 Phone 412 922 8850 Fax 412 922 9047 Europe YSI Life Sciences Xylem Longfield Road Tunbridge Wells Kent TN2 3EY UK Phone 44 1892 500400 Fax 44 1892 543115 Asia Smartec Scientific Corp 7F 6 No 12 Lane 609 Sec 5 Chung Hsing Road San Chung T...

Page 156: ...2 2010 3 rd Edition EN 61000 4 4 2004 A1 2010 2 nd Edition EN 61000 4 5 2005 2 nd Edition EN 61000 4 6 2008 3 rd Edition EN 61000 4 11 2004 2 nd Edition EN 61010 1 2010 3 rd Edition YSI Incorporated declares that the instrument specified above conforms to the essential requirements of the Directives and Standards specified above when installed and operated in accordance with specifications as set ...

Page 157: ... 2007 A1 2009 AS NZS 61000 3 3 2012 IEC 61000 4 2 2008 2 nd Edition IEC 61000 4 3 2006 A1 2007 A2 2010 3 rd Edition IEC 61000 4 4 2004 A1 2010 2 nd Edition IEC 61000 4 5 2005 2 nd Edition IEC 61000 4 6 2008 3 rd Edition IEC 61000 4 11 2004 2 nd Edition The tests verified that this device conforms to the Australian and New Zealand Electromagnetic Compatibility EMC requirements for generic products ...

Page 158: ...erference by one or more of the following measures Reorient the receiving antenna Relocate the computer with respect to the receiver Move the computer away from the receiver Plug the computer into a different outlet so that the computer and receiver are on different branch circuits If necessary the user should consult the dealer or an experienced radio television technician for additional suggesti...

Page 159: ...es Run Run Batch Rack ID Batch Name Multi Sample Batch Repeats Units Chemistries Status Module Printout Results Run Stat Monitor Data Plate View Date Filter Export Monitor View Date Filter Export Calibration Date Export Settings System Reports Sipper Fluid Det Display Brightness Sound Calibrate Auto Auto Cal Auto Flush Scheduler Screensaver Calibration 21 CFR 11 Users History Service Sipper Positi...

Page 160: ... Lactose 5 00 5000 500 0 50 14 62 342 Methanol 1 00 1000 100 0 10 31 25 32 Peroxide 6 0 60 600 60 0 06 17 65 34 Sucrose 5 00 5000 500 0 50 14 62 342 Xylose 20 0 20000 2000 2 00 133 3 150 Ammonium 0 50 500 50 0 05 27 8 18 Potassium 1 00 1000 100 0 10 25 6 39 1 If you are using a standard of a value not listed in the preceding table refer to the example conversions below to help calculate your unit ...

Page 161: ... 28 5 26 25 26 250 2625 2 63 76 8 Lactose 25 00 25 000 2500 2 50 73 1 342 23 75 23 750 2375 2 38 69 4 42 0 42 000 4200 4 20 456 0 Glycerol 40 0 40 000 4000 4 00 434 3 92 38 0 38 000 3800 3 80 412 6 2 63 2625 263 0 263 82 0 Methanol 2 50 2500 250 0 250 78 1 32 2 38 2375 238 0 238 74 2 26 25 26 250 2625 2 63 76 8 Sucrose 25 00 25 000 2500 2 50 73 1 342 23 75 23 750 2375 2 38 69 4 33 00 33 000 3300 3...

Page 162: ...1 0 40 146 L Glutamine 0 06 58 5 8 0 01 0 40 146 L Lactate 0 03 30 3 0 01 0 34 89 Lactose 342 Galactose 180 Glycerol 92 Methanol 0 05 50 5 0 01 1 56 32 Sucrose 0 10 100 10 0 01 0 29 342 Xylose 0 05 50 5 0 01 0 33 150 NOTE Use the values from the preceding tables only when calibrating with the appropriate YSI calibrator solution Choline 2772 Glucose 2776 or 2747 Ethanol 2790 Ethanol HC 7151 Sucrose...

Page 163: ...162 17 Appendix C Line Power Cord and Plug Wiring Figure 17 17 1 United Kingdom United States Europe ...

Page 164: ...actose 2703 Sucrose Membrane Kit 2725 Methanol Membrane Kit 2735 Glutamine Membrane Kit 2754 Glutamate Membrane Kit 2761 Xylose Membrane Kit 2786 Ethanol Membrane Kit 7140 Glycerol Membrane Kit 2974 Ammonium ISE 2975 Potassium ISE 2976 Reference Electrode 2736 Glutamine Standard 5 0 mmol L Calibrator 2755 Glutamate Standard 5 0 mmol L Calibrator 2767 Xylose Standard 20 0 g L Calibrator 2780 Sucros...

Page 165: ...bottles with caps and fluid detection cables 2934 Bottle Set Right Side Bottles and caps only 2935 Buffer Bottle Opaque For 2705 Buffer 2936 Bottle Rack Two Modules Left Side Includes bottles with caps and fluid detection cables 2937 Bottle Set Two Modules Left Side Bottles and caps only 2941 R24 Capped Vial Tray 24 position 2942 P96 Flat Bottom 96 Well Plates Case of 100 2943 P96 Round Bottom 96 ...

Page 166: ...annum Lane Yellow Springs Ohio 45387 USA 937 767 2769 800 659 8895 Fax 937 767 8058 527626 Support ysi com A527626H www ysi com 2016 06 2016 Xylem Inc YSI is a trademark of Xylem Inc or one of its subsidiaries ...

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