YSI 2900 series, Operation And Maintenance Manual

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8.2.9 L-Lactate
This is a direct reading of L-Lactate (L-Lactic Acid) in solution at the enzyme sensor. The enzyme L-Lactate Oxidase is
immobilized in the enzyme membrane.
L-Lactate + O
2 L-Lac Oxidase
H
2
O
2
+ Pyruvate
System Buffer YSI 2357
Calibrator Std YSI 2776
Linearity Std YSI 1530
Membrane YSI 2329
Membrane
Color Gray
Detection
Range 0.05–2.70 g/L
Calibration
Point
0.5 g/L
Linearity Check
Point 2.67 g/L
Sample Size
25
µ
L
End Point 30 sec
Precision
(CV,n=10) 2% or 0.03 g/L,
whichever is
greater
Linearity
±
2%
(0.05 to Cal Point)
±
5%
(Cal Point to Range
Max)
Typical Working
Life
14 days
Note: See Appendix B – Concentration
Unit Conversion if concentration unit
conversion is required.
Special Considerations:
•
If sample dilution is required, use reagent water.
•
D-Lactate is not a substrate for L-Lactate Oxidase.
Therefore, the 2900 Series cannot directly measure
D-Lactate. If you have a known racemic mixture of
lactates, the L-Lactate value multiplied by 2 should give
you the total lactate value.