YHLO BIOTECH UNION, Operation Manual

Page: 41 / 59

29
V2.3.0 OM-UNN valid from 15
th
April 2015
Fig.4-2 Calibration Curve
Fig. 4-3 Curve Correction

The procedure to re-calibrating the Calibration Curve
（
Point-to-Point
）
is as follows:

The optical density of the calibrator OD
cal
will be calculated by the formula OD
cal
= OD
450
- OD
630;

If OD
cal0
– TOD
cal
≤ OD
cal
≤OD
cal0
+ TOD
cal
, The calibration, OD
cal
will be accepted;
or the calibration will be cancelled and the error message will be displayed;

the K
cal
which is the correction Index will be calculated by the formula K
cal
= OD
cal
/ OD
cal0
;

The original Calibrator data points from the stored information from the barcode will be recalculated
according to K
cal
and the corrected curve will be stored.

The procedure to re-calibrating the Calibration Curve( 4PL ) is as follows:
OD2
OD3
OD4
OD5
OD1
C1 C2 C3 C4 C5
OD2
OD3
OD4
OD5
OD1
C1 C2 C3 C4 C5