Waters 2414, Overview And Maintenance Manual

Page: 91 / 130

June 11, 2015, 715004754 Rev. A
Page 91
5.7 Powering-off
Recommendation:
Do not power-off the detector unless you are storing it.
If you are not storing the detector, set the flow rate to 0.1 mL/min, and keep the
pump or solvent delivery system operating to minimize the amount of time the
detector needs for re-equilibration when you use it again.
5.7.1 Removing buffered mobile phase
To remove mobile phase from the fluid path of the 2414 RI detector:
1. Replace the buffered mobile phase with 100% HPLC-quality water and flush the
system for 10 minutes at 2 mL/min.
2. Replace the 100% water mobile phase with a solution of 90% methanol:10%
water, and flush the system for 10 minutes at 2 mL/min.
3. Cap the flow ports, or, for between 5 and 10 minutes, dry the flow cell using dry,
oil-free nitrogen, helium, or air.
Follow the recommended procedures for injector purging and priming for the pump in
use with your HPLC.
Power-off any peripheral devices before shutting down the detector. To power-off the
detector, press the power switch located at the upper-left front corner of the unit.
Notice:
To avoid harming the column, remove it before flushing the system
with a solvent that is incompatible with the column's chemistry.