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7.2 OFN Acquisition
Use the Inject Single Sample option to make a 1 µL manual injection of test
standard 03-931127-02 (split style injection) or a 10:1 diluted test standard
(splitless pressure pulse style injection). Use a lower air gap to avoid sample loss
through the tip of the needle. Ensure the syringe plunger is not forced out of the
syringe barrel if a Pressure Pulse is used in the method.
7.3 Library Search and Signal-to-noise Calculation
After acquiring the data file, calculate the S/N and run a library search. Use this
opportunity to demonstrate data analysis to the customer. The .sms data file can
be loaded into Mass Spectra Data Review (MSDR) using the quick load feature
shown in Figure 5.2. Refer to Section 5 of this document for assistance in plotting
the m/z 272 ion from the OFN acquisition, calculating the RMS signal-to-noise,
and achieving a passing library search. Be sure to use 5 point Savitsky Golay
smoothing on the chromatogram and background correction on the spectrum. A
passing signal-to-noise calculation and library search are shown in Figure 7.1.
Note that OFN is found in the Tutorial Library.
Figure 7.1
Summary of Contents for 4000 GCMS
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