Varian 4000 GCMS, Installation Procedures Manual

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Contents
Introduction...............................................................................................................................5
Section 1. System Unpacking / Checking / Set Up ................................................................7
1.1 Unpacking / Parts Checking .................................................................................................................................7
1.2 System Set Up On Bench.....................................................................................................................................8
1.3 GCMS Plumbing and Purging ..............................................................................................................................9
1.4 Column Installation Into the MS............................................................................................................................9
Section 2. GC/MS Power Up ..................................................................................................11
2.1 Initial Power Up ..................................................................................................................................................11
2.2 Quick Check of Air / Water .................................................................................................................................16
2.3 Checking for High Mass Noise ...........................................................................................................................20
2.4 System Conditioning...........................................................................................................................................21
Section 3. Preliminary Tuning ...............................................................................................23
3.1 System Monitoring..............................................................................................................................................23
3.2 RF Ramp Adjustment .........................................................................................................................................24
3.3 Dipole Adjustment ..............................................................................................................................................26
3.4 Air Water Check .................................................................................................................................................28
3.5 Cal Gas Adjustment............................................................................................................................................29
Section 4. Auto Tuning...........................................................................................................31
4.1 Internal Auto Tuning ...........................................................................................................................................31
4.2 External Auto Tuning ..........................................................................................................................................32
Section 5. Achieving Internal EI Specifications ...................................................................35
5.1 Methodology .......................................................................................................................................................35
5.2 OFN Acquisition..................................................................................................................................................36
5.3 Library Search and Signal to Noise Calculation.................................................................................................36
5.4 Customer Familiarization....................................................................................................................................49
Section 6. Achieving Internal CI Specifications...................................................................51
6.1 Install Liquid CI ...................................................................................................................................................51
6.2 Adjust Liquid CI Reagent Flow ...........................................................................................................................52
6.3 Methodology .......................................................................................................................................................53
6.4 BZP Acquisition ..................................................................................................................................................54
6.5 Signal-to-noise Calculation.................................................................................................................................54