Portable Optical Tweezers Kit
Chapter 9: Troubleshooting
Page 86
MTN024417-D02
Chapter 9 Troubleshooting
•
When the stage is moved, the sample quickly moves out of focus and loses the
bead.
We have seen this behavior when the screw on the locking plate is tightened too
much.
•
The camera image is stuck/frozen.
If the image is stuck (framerate in status bar not counting), close the program.
Take the USB out and plug it back in. Restart the ThorCam software. Enter the
settings and reduce the pixel clock to 8 - 10 MHz, exposure to 100 ms, and restart
the stream from the camera.
•
Actuating the knob on the KLD101 does not change the laser diode current.
Make sure you are in the “Const I” mode. Make sure all settings are correct,
especially the anode grounding. If the knob still does not show an effect, unplug
the USB from the PC, turn the power of the KLD101 off and back on again.
•
All settings are correct, but the laser diode still doesn’t emit light.
Switch of the KLD101, then remove the laser diode from its mount and reinsert
it (see Section 5.1.1).
•
KDC101 controller shows the message “Overload”.
This message means that the motor was driven to the maximum extent. First, try
to switch the KDC101 off and back on again. Then, move the stage back. If this
does not work, you need to remove the motor from the MT1(/M) stage. Loosen
the screw that holds the barrel and remove the motor
while holding both parts
of the stage to avoid rapid retraction and potential damage
. When the motor
is removed, turn the KDC101 off and back on. Then, actuate the motor towards
the middle position. Afterwards, insert the motor back into the MT1(/M).
•
When the stage is actuated, the camera image moves at an angle.
Make sure the camera is mounted parallel to the cage system.
•
The optical tweezers don’t trap beads.
o
Check your laser diode’s current. It should be about 95 mA.
Summary of Contents for EDU-OT3
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