THORLABS EDU-OT3, User Manual

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Portable Optical Tweezers Kit
Table of Contents
Chapter 1 Safety ............................................................................................ 1
1.1. Warning Symbol Definitions ..................................................... 1
1.2. Laser Radiation Warning ......................................................... 1
Chapter 2 Product Description .................................................................... 2
Chapter 3 Principles of Optical Tweezers ................................................... 4
3.1. Dipole Approach in the Rayleigh Scattering Regime R <<
λ
....... 4
3.2. Geometrical Optics Approach in the Mie Regime R >>
λ
........... 6
Chapter 4 Kit Components ......................................................................... 16
4.1. Trapping Laser Source ............................................................. 16
4.2. Beam Expander ....................................................................... 17
4.3. Right-Angle Mirrors ................................................................ 18
4.4. Sample Positioning System ..................................................... 19
4.5. Microscope ............................................................................. 20
4.6. Additional Components ........................................................... 22
4.7. Included Hardware ................................................................. 23
4.7.1. Imperial Kit .............................................................................................. 23
4.7.2. Metric Kit ................................................................................................. 24
Chapter 5 Setup and Adjustment ............................................................... 25
5.1. Assembly ................................................................................ 25
5.1.1. Trapping Laser Source ............................................................................ 26
5.1.2. Beam Expander ...................................................................................... 28
5.1.3. Microscope .............................................................................................. 33
5.1.4. Sample Positioning System .................................................................... 40
5.2. Controller and Software Setup ................................................ 44
5.2.1. LED Controller ......................................................................................... 44
5.2.2. Laser Controller ....................................................................................... 44
5.2.3. Camera ................................................................................................... 46
5.2.4. Sample Positioning Stages ..................................................................... 50
5.2.5. Kinesis Settings ....................................................................................... 53
5.3. Camera Adjustment ................................................................ 54