Portable Optical Tweezers Kit
Chapter 5: Setup and Adjustment
Page 54
MTN024417-D02
5.3. Camera Adjustment
The objective used in this kit is infinity-corrected. That is a great advantage and eases the
adjustment significantly. It means that the light from the objective’s focal plane leaves the
objective effectively parallel. This plane can be mapped onto a camera with a lens at an
almost arbitrary distance to the objective. To find the correct distance between lens and
camera chip, one only needs to map a distant object onto the chip. Proceed the following
way:
Step 1: Preparation
•
Remove the camera segment from the microscope (that includes all parts starting
from the camera until the cage plate above the beamsplitter). For that, unscrew
the cage locking screws from the CP33T(/M) and loosen the screw on the
C1498(/M) post clamp.
•
Start the camera software.
•
Open the settings and set the gain to maximum.
Step 2: Lens Adjustment
•
Aim the camera at an object more
than 4 m away. Make sure the
room is well lit or activate the gain
boost in the camera settings
(beneath the regular gain). You
could also increase the exposure
time.
•
Move the lens in the cage segment
until you see a sharp image of the
object. Below is a sample image of
a clock at 4 meters distance.
Step 3: Installation of the camera
•
Place the camera segment back into the microscope. Tighten the cage locking
screws of the CP33T(/M) and fix the screw on the C1498(/M) post clamp.
Summary of Contents for EDU-OT3
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