Portable Optical Tweezers Kit
Chapter 5: Setup and Adjustment
Rev B, February 15, 2022
Page 61
Step 4: Camera Position
•
Depending on the angle of the optical elements, the laser spot may not be in the
center of the camera image. In fact, this is hardly ever the case and does NOT
constitute bad alignment.
•
You can move the laser spot closer to the center of the camera image by
physically moving the camera. The camera’s lens tube is mounted on an SPT1C
slip plate that allows travel of ± 1 mm in each direction. Loosen the two screws at
the top, move the slip plate with the attached lens tube/camera and fix the screws
again.
•
In rare cases, you may initially not see a laser spot in the camera image at all. In
these cases, you may find it within the travel range of the SPT1C. Make sure you
have the correct plane by getting the beads in focus and move the SPT1C’s slip
plate to find the laser spot.
Step 5: Trap a Bead
•
Move the stage using the wheels on the controller cubes. Move the sample so
that a bead comes to rest under the laser spot, see Figure 45.
•
When the bead is close to the spot, you should see it getting pulled into the focus.
•
Move the sample around by moving the stage. The bead should stay trapped in
the laser focus while the sample is moved.
2 µm Bead is Trapped in the Laser Focus
Summary of Contents for EDU-OT3
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