Test Procedures
Page 36 of 60
M1393R0
OQ/PQ Operating Instructions
The programs used for the gradient accuracy tests are listed in TABLE 4-4 and TABLE 4-5.
TABLE 4-4
Program for the Gradient Accuracy Test on a NAN Configuration
Time
[min]
%A
%B
Wavelength
[nm]
0.00 100
0
254
10.00 100
0
“
10.01 95
5
“
20.00 95
5
“
20.01 50
50
“
30.00 50
50
“
30.01 5 95 “
40.00 5 95 “
40.01 0 100 “
50.00 0 100 “
50.01 100
0
“
80.00 100
0
“
TABLE 4-5
Program for the Gradient Accuracy Test on a CAP Configuration
Time
[min]
%A
%B
Wavelength
[nm]
0.00 100
0
254
5.00 100
0
“
5.01 95
5
“
10.00 95
5
“
10.01 50
50
“
15.00 50
50
“
15.01 5 95 “
20.00 5 95 “
20.01 0 100 “
25.00 0 100 “
25.01 100
0
“
50.00 100
0
“
Experimental conditions for the step gradient test for nano- and capillary LC systems are
listed in TABLE 4-6.
TABLE 4-6
Experimental Conditions for the Step Gradient Test.
Parameter
Nano Configuration
Capillary Configuration
Solvents
A: HPLC – Water
B: HPLC – Water with Acetone
C: 100% ACN + 0.1% FA (a)
D: 100% Water + 0.1% FA
Acetone concentration of
Solvent B
0.8% 0.3%
Detection wavelength
254 nm
254 nm
Run time
80 min
50 min
Flow rate
0.4
µ
l/min 4.0
µ
l/min
Note:
a) The acetonitrile is used to perform a wash step during the
FAMOS test procedure 4.8.
