Test
Procedures
OQ/PQ Operating Instructions
M1393R0
Page 41 of 60
4.14
Completing and Printing the OQ/PQ Report
4.14.1
Result of the Gradient Accuracy Test
The observed delay time may be slightly different than the expected delay time indicated in
(TABLE 4-7) because of small differences in the tubing size due to the manufacturing
process. Due to this the calculated result of the ‘Gradient Accuracy‘ test (Section 4.10)
may be wrong and the system may not pass the test. In such a case the delay times
should be checked and adjusted if necessary. This applies for the 5%, 50%, 95% and
100% steps or if the result is not within the specifications only.
TABLE 4-7
Predefined Step Delay Time
Step
NAN
CAP
5 %
17.0 .. 18.0 min
8.0.. 9.0 min
50 %
27.0 .. 28.0 min
13.0 .. 14.0 min
95 %
37.0 .. 38.0 min
18.0 .. 19.0 min
100 %
47.0 .. 48.0 min
23.0 .. 24.0 min
To change the delay time:
a)
Open the UV-trace obtained for the third step gradient (out of 3)
b)
Measure the retention times corresponding to the middle of 5%, 50%, 95% and 100%
steps.
c)
Select the report layout. Open the ‘Pump Gradient’ sheet and go to line 137.
d)
Make sure to modify the correct columns only (FIGURE 4-14):
•
D, E, F, G for capillary (CAP) configurations
•
J, K, L, M for nano (NAN) configurations
Note: The first part is dedicated to cap LC and the other to Nano LC.
FIGURE 4-14 Section of the Report Table to be modified
e)
Double click on each column to open the
Properties Report Column
