Test
Procedures
OQ/PQ Operating Instructions
M1393R0
Page 37 of 60
4.10.1
Performing the Test
a)
Setup the UltiMate system and the FAMOS Microautosampler as discussed in Sections
3.2.2 A and 3.2.2 B and TABLE 4-6. Degas the solvents properly.
b)
The four channels should be well flushed using the Purge function of the Micropump.
c)
Start the flow delivery of channel B from the CHROMELEON
panel and monitor the
baseline until it is stable.
d)
After equilibration start the sequence ‘XQ_6_ Gradient Formation Test_NAN’ (or ‘_CAP’,
respectively).
Note: If the maximum absorbance observed during this test is greater than the highest
absorption monitored during the linearity test (4.7), dilute B (or D) with mobile phase A so
that the signal remains on scale when this test is run.
e)
Verify that the results meet the acceptance criteria (TABLE 2-3).
f)
Repeat steps (a) and (h) for the Gradient Pump 2 of the UltiMate Dual Gradient system
(if applicable).
