Process
Page 16 of 60
M1393R0
OQ/PQ Operating Instructions
c)
Open the CHROMELEON Server Configuration. Load the server configuration file and
check the COM port settings. Modify the configuration if necessary.
3.2.1 B
Modifying Sequences and Programs
It is recommended that you do not modify the sequences provided on the CD ROM
‘IQOQPQ on UltiMate
™
(Plus) Systems’ (P/N 163935) unless it is absolutely necessary.
Changing them may have an impact on the links with the report. However, it may be
necessary to make modifications to correspond to the available hardware. If changes are
made, take care that the sample names and numbers are not modified.
3.2.1.B.1
Choosing to perform OQ or PQ
The sequences required to perform the OQ/PQ on a Nano LC system have the extension
‘_NAN’ in their names (e.g. XQ_6_Gradient Formation Test_NAN.seq). Sequences for a
capillary LC system have the extension ‘_CAP’ (FIGURE 3-2).
FIGURE 3-2
Modifying the Sequence Names
All sequence names start with the prefix ‘XQ_’. Depending on the test that is to be
performed, change ‘XQ’ to ‘OQ’ or ‘PQ’ (FIGURE 3-2). This will select the right limits for
the report file. If you do not modify the name, the PQ limits will be selected.
3.2.1.B.2
Flow Sensor Support and CRP Value
All sequences are prepared to support the flow sensor option. If the system to be checked
does not include the flow sensor, all relevant programs must be adjusted:
To change the programs for a using a fixed CRP value, identify the ‘CRP’ or ‘CalibrateCRP’
command in the program and remove the semicolon from the ‘CRP’ line and place it on the
‘CalibrateCRP’ line as presented below:
•
Flow Sensor Support:
;CRP = 625
CalibrateCRP When=BeforeFirstSample
•
Using a fixed CRP Value:
CRP = 625
;CalibrateCRP When=BeforeFirstSample
3.2.1.B.3
FAMOS Cooling Option
All sequences are prepared to support the cooling option of the FAMOS. If the system to
be checked does not include the cooling option, all relevant programs must be adjusted:
To change the programs for instruments for which do not have this option installed,
identify the ‘Sampler_TempCtrl’ command and in the programs and add a semicolon to this
line and the 3 following lines as presented below:
