Thermo Scientific TSQ Altis, Hardware Manual

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D
Setting Up Sample Introduction Techniques
Setting Up High-Flow Infusion Without an Autosampler
Thermo Scientific TSQ Altis, Quantis, and Fortis Hardware Manual 137
Setting Up High-Flow Infusion Without an Autosampler
The high-flow infusion method uses an LC Tee union to direct the solvent flow from the
syringe pump into the solvent flow produced by an LC pump. The combined solvent flow
goes through the divert/inject valve into the API source. Use this infusion method to perform
experiments at a higher flow rate with an LC system. The high-flow infusion method allows
you to optimize the source parameters (such as sheath gas and vaporizer temperature) at the
flow rate and mobile phase composition of the assay.
Before you begin, complete the procedures in Appendix B, “Setting Up the Syringe with the
Syringe Pump.”
After you set up the Tee union, connect the API source.
• For H-ESI or low-flow H-ESI, see “Connecting the Sample Flow to the H-ESI Source”
on page 51
• For APCI, go to “Connecting the Sample Flow to the APCI Source.”
1. Fill the syringe with your sample (1 in Figure 71 ) and connect it to the syringe pump,
(not shown).
2. Using red PEEK tubing (2) and fittings, connect the syringe union adapter to the base of
the Tee union (3). This is the infusion line.
3. Use a fingertight fitting to connect a piece of red PEEK tubing (not shown), to one side
of the Tee union (3). This adds the flow from the LC pumps to the flow from the syringe
pump.
4. Using red PEEK tubing and fittings (5), connect the open end of the Tee union to the
API source at Port 6. This brings the flow to the MS.
5. (Not Shown) Using a Rheodyne fitting, connect one end of the Teflon tubing to Port 3.
Place the other end into an appropriate waste container.
Note The inject/divert valve Figure 72 is an example. The valve in your system may be
different.