D
Setting Up Sample Introduction Techniques
Setting Up Loop Injections for Flow-Injection Analysis
138
TSQ Altis, Quantis, and Fortis Hardware Manual
Thermo Scientific
Figure 71.
HPLC infusion Tee valve
Setting Up Loop Injections for Flow-Injection Analysis
When you have a limited amount of sample, use manual loop injections with or without an
LC column.
This technique requires attaching a sample loop, an injection port fitting (needle port), and
an LC pump to the divert/inject valve, and then connecting the valve to the API source. With
the valve in the Load position, you use a syringe to load sample through the injection port
fitting into the sample loop, and then switch the position of the inject valve to the Inject
position. Switching the valve to the Inject position allows the solvent flow from the LC pump
to backflush the sample out of the loop and into the API source.
1. Connect a sample loop (1) across Ports 1 and 4 of the valve.
2. Connect a loop filler (needle port) (2) to Port 2.
No.
Description
1.
Syringe and union
2.
Red PEEK tubing infusion line
3.
Tee union
4.
(not shown) PEEK tubing from LC
5.
Red PEEK tubing connecting the Tee valve to Port 6 (to the MS)
1
3
5
2
4
