D
Setting Up Sample Introduction Techniques
Setting Up Injections Using the Autosampler
Thermo Scientific
TSQ Altis, Quantis, and Fortis Hardware Manual
139
3. Use a Rheodyne fitting to connect one end of the Teflon tubing (3) to Port 3. Place the
other end in a waste container, not shown.
4. Use red PEEK tubing (5) to connect Port 5 (fingertight fitting) to bring in the LC pump
flow.
5. Connect the red PEEK tubing (6) from Port 6 to the API source.
• To connect to the H-ESI source go to in
• To connect to the APCI source go to
“Connecting the Sample Flow to the APCI
Figure 72.
Injector valve set up
Setting Up Injections Using the Autosampler
You can use the software to automatically inject a set of sample from the autosampler. In a
typical LC/MS experiment, solvent flow goes through an LC column to separate the
compounds of a mixture before they are directed into the API source.
1. Use red PEEK tubing,
, and a Rheodyne fitting to connect Port 2 of the
divert/inject valve to one of the following:
• The outlet of the autosampler (not shown)
• The outlet of a column that is attached to the autosampler (not shown).
No.
Description
1.
Sample loop (across Port 1 and 4)
2.
Sample input Port 2
3.
Teflon tubing for waste Port 3
4.
Sample loop (across Port 1 and 4)
5.
Red PEEK from LC pump Port 5
6.
Red PEEK to MS Port 6
3
2
1
5
4
6
3
2
1
6
5
