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Thermo Scientific Spin Tissue Processor Microm STP 120

5

Microm International GmbH

part of Thermo Fisher Scientific

Otto-Hahn-Str. 1A

69190 Walldorf / Germany

387 718 - English

Dear valued Customer,

Thank you for buying this instrument.

Before putting the instrument into operation, please read these
operating instructions carefully to familiarize yourself with its
proper operation and function. Non-compliance with these
instructions means that you are invalidating the standards of safety
and use for which this instrument was designed.

The STP 120 is an efficient spin tissue processor with carousel
system for embedding specimens in paraffin.

Only skilled or specially trained personnel must operate the
instrument. The listed and marked safety measures as well as the
regulations and hygiene measures of your respective lab must
strictly be observed.

Ser. No………………………………………..

Please enter the serical number, which is placed on the type plate
on the rear side of the instrument. This way, questions and service
can be handled faster.

Operating instruction no.: 387718

Issued on April 18

th

, 2011

Microm International GmbH

part of Thermo Fisher Scientific

Otto-Hahn-Str. 1A

69190 Walldorf

Germany

Phone:

+49.(0)6227.836-0

Fax:

+49.(0)6227.836-111

Email:

[email protected]

Internet: www.thermoscientific.com

Intended use and safety
precautions

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Summary of Contents for STP-120

Page 1: ... Tissue Processor Microm STP 120 1 Microm International GmbH part of Thermo Fisher Scientific Otto Hahn Str 1A 69190 Walldorf Germany 387 718 English Thermo Scientific SPIN TISSUE PROCESSOR Microm STP 120 INSTRUCTION MANUAL ...

Page 2: ...ue Processor Microm STP 120 2 Microm International GmbH part of Thermo Fisher Scientific Otto Hahn Str 1A 69190 Walldorf Germany 387 718 English SPIN TISSUE PROCESSOR Model STP 120 INSTRUCTION MANUAL Version 2 30 English 04 2011 ...

Page 3: ...emplacement Transport and unpacking Emplacement Connection Control panel Programming Basket cycle Example of a process Entering the program Starting the program Delayed start Stopping the program End of the program Temperature display End of process display Setup Menu Changing the language Changing the date and time Working with two baskets Power failure Emergency Movements Maintenance Cleaning Ch...

Page 4: ...ng the unit This manual uses the following symbols DANGER Special warning messages to prevent harm to persons and or serious damage to equipment For your own safety please observe these instructions carefully CAUTION WARNING Special precautionary measures to prevent damage to equipment For a long lifetime of the equipment please observe these instructions carefully NOTE Special instructions regard...

Page 5: ...nt spin tissue processor with carousel system for embedding specimens in paraffin Only skilled or specially trained personnel must operate the instrument The listed and marked safety measures as well as the regulations and hygiene measures of your respective lab must strictly be observed Ser No Please enter the serical number which is placed on the type plate on the rear side of the instrument Thi...

Page 6: ...tment of components should only be carried out by authorised trained maintenance staff Disconnect the unit from the mains power supply before opening or removing any covers DANGER RISK IN EXPLOSIVE ENVIRONMENT The instrument must not be operated in the presence of flammable gases DANGER RISK OF RADIOACTIVE RADIATION When working with radioactive specimens all procedures applicable to protection ag...

Page 7: ...safety Manual 1984 of the World Health Organization When working with radioactive contaminated material appropriate safety and disinfection measures must be carried out According to the rules and regulations concerning the handling of radioactive contaminated material of the respective laboratory safety clothing e g particle mask gloves protective shoe covers must be worn Separate taking back of e...

Page 8: ...y will expire in case of improper or wrong use of the instrument and in case the warning and precautionary messages are not observed Thermo Fisher Scientific Microm International GmbH is not liable for any occurring damage Once the guarantee period has expired a maintenance contract should be signed to ensure the unit is kept in optimum operating condition For more information about maintenance co...

Page 9: ...g Electrical installation Rated Voltage 10 100 120 V 1 7 A 50 60 Hz 220 240 V 0 9 A 50 60 Hz Fuses 115 V 2 x T4AH 230 V 2 x T2AH Battery Nickel Cadmium 12V 600 mA Overvoltage category 2 Pollution degree II Sound pressure 70 dBA Capacity Reagent stations Number of reagent vessels 10 9 if 3 are for paraffin Volume per reagent vessel 1 8 L Baskets for the specimen holders Number of baskets 1 option o...

Page 10: ...ation time per station from 1 s to 99 h 59 m Rotation stirring selectable Vertical shake selectable Centrifugation selectable Centrifugation time selectable Program start delay selectable without time limit Environmental conditions at a max rel humidity of 60 without condensation Temperatures from 5 to 40 C Storage temperature range from 20 C to 50 C Altitude up to 2000 m M S L Floor loading requi...

Page 11: ...d control before being packed Open the packing carefully and make sure that the machine has not suffered any damage during transport If any damage is observed immediately report this fact to the distributor and the company insuring the transport Remove any support or brace holding the machine inside the packing box CAUTION Two people are required to remove the carrousel from the packing box Take c...

Page 12: ...machine Refer to sizes and weights in the section on characteristics WARNING When selecting the emplacement for the machine remember that the Main ON OFF switch is located at the rear and that it must be accessible at all times Manual rotation of the whole unit Apart from the automatic rotating system for the processor cover the base of the unit is also fitted with wheels This enables the whole un...

Page 13: ...wer cable make sure that the machine is adjusted to operate at the supply voltage The factory settings for the power supply are for operation with 220 240 V If the unit is operated with 100 120 V open the cover of the power plug remove the fuse holder and invert its position so that the visor shows 115 V the correct operating voltage The fuses should also be replaced by others corresponding to rat...

Page 14: ...wed by FILTER CHANGE XXXX HOURS Refer to Changing the filter and then C10 C11 C12 16º 16º 16 indicating the temperature of the paraffin baths NOTE These messages may appear in a language other than your own If you want to change the language now refer to section Changing the language Furthermore the date and time perhaps do not coincide with those in your country If you want to adjust the time ref...

Page 15: ...dorf Germany 387 718 English Now you can place the reagent vessels and paraffin baths in position Connect the paraffin baths CAUTION Before connecting or disconnecting the paraffin baths make sure that the unit is disconnected from the power supply by turning off the main switch located at the rear of the unit Now you can turn the unit on again ...

Page 16: ...ss reagent vessels Depending on the processor model the reagent vessels may be either glass or aluminium The marks in the centre indicate the maximum fill level for the reagent or paraffin The dimple on the bottom allows the vessel to be correctly centred in position DANGER Under no circumstances should the maximum fill level be exceeded as the process will not be performed correctly and reagent o...

Page 17: ...ng the unit by hand CAUTION Before connecting or disconnecting the paraffin baths make sure that the unit is disconnected from the power supply by turning off the main switch located at the rear of the unit CAUTION Always use the following connections n the connection marked with the number 10 only if a third paraffin bath has been installed in position 10 n the connection marked with the number 1...

Page 18: ...onnector When fitting the baskets make sure that the four studs fit into the four grooves on the edge turn the basket as far as possible inside the groove and allow it to rest on the pivots of the basket holder see figure Before beginning to process tissues you should become familiar with the handling and programming of the machine Design a small test program and perform some manual movements with...

Page 19: ... more information refer to section Power failure START Key to start the automatic operating cycle program When the unit is operating in automatic mode the pilot beside the key is lit STOP Key to stop the automatic operating cycle If this key is pressed the microprocessor asks for confirmation of whether the process is to be aborted Once the stop is confirmed the program is cancelled and the machin...

Page 20: ...power failure the basket will not descend into the paraffin bath even though this is programmed until the default safety period has elapsed When testing the programming skip paraffin bath positions 11 and 12 and 10 if working with two baskets by assigning them a time period of 00 00 If you program a time period of more than 00 00 in positions 11 and 12 10 if working with three paraffin baths the s...

Page 21: ... position or changing reagent vessel Basket in central position or centrifugation ƒ Basket in lower position or immersion Downward movement The basket does not turn Stirring by rotation for time T1 programmable in each reagent vessel Reversal of the direction of rotation each minute with programmable rotation speed 0 60 70 rpm at each reagent vessel Only if SHAKE vertical shake is activated at eac...

Page 22: ...ramming display It has the following meaning 0 without rotation stirring 1 rotation stirring at 60 rpm 2 rotation stirring at 70 rpm In cases 1 and 2 the direction of rotation of the motor is reversed every 60 seconds Enter the program when the machine is connected to the power supply and in the stop position Make sure that n the vessels contain the reagents indicated on the list n the basket hold...

Page 23: ...Minutes A Is the stirring rate 0 no stirring 1 stirring at 60 rpm 2 stirring at 70 rpm Pulse ENTER to assign the values to reagent vessel 01 Use the UP or DOWN keys to increase or reduce the value and ENTER to confirm the value and go to the next option If a reagent vessel is assigned a time of 00 00 the carrousel will not immerse the basket in the corresponding reagent The option of vertical shak...

Page 24: ... is required press the SHAKE key The key indicator lights up 5 Only if working with a double basket press the x2 key The pilot beside the key will light 6 Press the START key to begin the program The display suggests executing program P 01 7 If a program other than 01 is to be used press the UP or DOWN keys to modify the option 8 Start the program immediately by pressing START again The program wi...

Page 25: ...ssed to begin a program immediately the carrousel will automatically move to reagent vessel 1 before beginning the process Under normal operating conditions the program will stop in paraffin bath 12 and issue a warning beep every five seconds If the program being run was P 01 the screen will display the following message Press the STOP key to stop the program Then press the key to raise the basket...

Page 26: ...me during the process by pressing the DOWN key The screen will display the temperatures of each of the vessels for a period of 3 seconds The expected time for completing the process can be consulted at any time by pressing the UP key The screen displays the expected date and time for completion of the process for a period of 3 seconds Temperature display C10 C11 C12 55 C 54 C 57 C End of process d...

Page 27: ... the pilot light located beside it If the language has been correctly configured refer to Changing the language and Changing the date and time The screen will display the following message The table below summarises the use of the keyboard in the SETUP MENU Key Action PROGRAM Enter EDIT PROGRAM mode Press the UP or DOWN key to access the SETUP MENU Key When the text displayed Does not flash Flashe...

Page 28: ...te and time 3 1 DAY 00 to 31 3 2 MONTH 00 to 12 3 3 YEAR 2000 to 2999 3 4 HOUR 00 to 24 3 5 MINUTE 00 to 59 4 FILTER Enables the values associated with the filter to be queried and adjusted 4 1 FILTER TIME 0001 to 9999 Theoretical hours of filter service life according to the manufacturer 4 2 CHANGE FILTER 0001 to 9999 Remaining hours of life since the last change Reset 4 3 FILTER RESET Caution Co...

Page 29: ... DOWN keys then allow access to other Setup options 2 Temperature Units 3 Clock 4 Filter 5 Paraffin temperature 6 Timers Step 5 Press ENTER to display the first option 1 1 Castellano Press the DOWN key until the desired language appears When it appears press ENTER The screen will display a flashing N No Press UP to change it to a Y Yes Press ENTER again to confirm the option Access the SETUP MENU ...

Page 30: ... 1A 69190 Walldorf Germany 387 718 English When the screen displays the value to be changed in this example the hour press ENTER to access the value which will begin flashing and then use the UP and DOWN keys to make appropriate changes Once correctly adjusted press ENTER to confirm the value and go to the next option 3 4 HOUR 10 ...

Page 31: ...o baskets remember to press the x2 key before starting the program with the START key this will light the indicator located beside the x2 key Otherwise the second basket optional will be erroneously submerged in the reagent in reagent vessel 1 when the carrousel moves to position 12 A very important feature of the SPIN TISSUE PROCESSOR is its capacity to react in the event of a power supply failur...

Page 32: ...damage of the transportation lift device The re melt timer may be reset to enable immediate access to the cassettes as soon as the user assures himself of the fact that the paraffin is in liquid condition and mechanical blockages are avoided Depending on the software version two individual buttons have to be pushed at the same time The actual software of your device is indicated for 4 seconds ever...

Page 33: ...it except the paraffin baths is being operated by the emergency battery power supply Under these circumstances it is possible to move the basket holder with the and keys to extract it should it be necessary to abort the process Once the specimen holder basket is removed the carrousel should be lowered again with the key to close the reagent vessels Press the STOP key to cancel the process that has...

Page 34: ...he power supply at the Main ON OFF switch DANGER When performing any maintenance or cleaning operations always bear in mind that the reagents used may be highly inflammable and that the paraffin baths may reach extremely high temperatures n Do not use reagents that contain acetone or xylol or any abrasive powders n Always clean the unit with domestic detergents without any abrasive additives n Use...

Page 35: ...ystem controls the life of the filter in hours When starting the unit the following message is displayed The value displayed in the example 400 hours is the time remaining for the filter to complete its service life The filter should be changed when this counter displays 0000 HOURS Change the filter by removing the rear cover and proceeding as shown in the figure below After changing the filter th...

Page 36: ...everal hours to allow the battery to charge itself If the battery does not recharge and the alarm message does not disappear the battery should be replaced with a new one Change the battery by accessing the rear part of the unit and proceeding as shown in the figure Place the new battery in position and reassemble the unit by proceeding in the reverse order Replacing the battery 1 Remove the six 6...

Page 37: ...at measures the temperature of the indicated station Make sure that the paraffin bath is connected to the correct connector Refer to connecting paraffin baths CHANGE FILTER The working life of the filter has expired Refer to Changing the filter SAMPLES OUT An attempt is being made to program a delayed start with the carrousel not lowered Place the carrousel in the start position basket submerged i...

Page 38: ...the programs in the unit and restores the default factory settings After this operation the basket holder must be moved using the keys and the carrousel moved to position 1 using the key Refer to section Entering the program to create new processing programs The SPIN TISSUE PROCESSOR may be used with the reagents that are shown in the list below Fixing Formalin buffered or unbuffered Trinitropheno...

Page 39: ...6 111 Email info dxd dewal thermofisher com Internet www thermoscientific com Caution Any shipping of the instrument requires original packaging materials Damages caused by shipping with non conform packaging are not covered by the manufacturer warranty Any damage repairs resulting in non conforming package are fully charged to the sending party We reserve the right depending on seriousness of dam...

Page 40: ...in the waste management The marking of the product and or the respective literature indicates that after its final shutdown it must not be disposed of together with ordinary domestic waste Please dispose of your instrument separately from other waste to not harm our environment and or human health by uncontrolled waste disposal Recycle your instrument to support the sustainable recycling of materi...

Page 41: ...isconnection range above and below the assigned temperature 1 2 MAX TEMPERATURE 50 to 70 70 C Temperature limits that can be selected by the user from the SETUP MENU 1 3 MIN TEMPERATURE 50 to 70 50 C 1 4 PARAF ACTIVE WITH TIMER Y N Y Enables keeping the paraffin baths hot during delayed start 2 FAN refer to the diagram below 2 1 OPERATING TIME 0000 to 9999 180 Time in seconds that the fan is conne...

Page 42: ...rt of Thermo Fisher Scientific Otto Hahn Str 1A 69190 Walldorf Germany 387 718 English SPIN TISSUE PROCESSOR Program Record Sheet Program number 01 to 10 Comments Written by Date Reagent vessel Reagent Time HH MM Stirring 0 1 2 Notes 1 2 3 4 5 6 7 8 9 10 11 Paraffin 12 Paraffin Comments ...

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