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Thermo Scientific SRD-3200, Operating Instructions Manual

Introducing the Thermo Scientific SRD-3200, an innovative laboratory instrument designed for precise measurements. Ensure optimal usage with our comprehensive Operating Instructions Manual, available for free download at manualshive.com. This manual provides step-by-step guidance, guaranteeing accurate and efficient operation of your SRD-3200, maximising productivity and results.

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UltiMate 3000 Series:  

SRD-3x00 Solvent Racks  

 

Operating Instructions  

Page 

i 

Table of Content 

1

 

Introduction ................................................................................................................... 1

 

1.1

 

How to Use this Manual ............................................................................................ 1

 

1.2

 

Safety Information ..................................................................................................... 2

 

1.2.1

 

Symbols on the Module and in the Manual ...................................................... 2

 

1.2.2

 

Safety Precautions ............................................................................................ 3

 

1.2.3

 

Consignes de Sécurité

 .................................................................................. 6

 

1.3

 

Intended Use .............................................................................................................. 9

 

1.4

 

Federal Communications Commission (FCC) Note ................................................ 10

 

2

 

Overview ...................................................................................................................... 11

 

2.1

 

Unit Description ....................................................................................................... 11

 

2.2

 

SRD Configurations ................................................................................................. 11

 

2.3

 

Front Panel Elements ............................................................................................... 13

 

2.4

 

Rear Panel ................................................................................................................ 14

 

2.5

 

Fluid Connections .................................................................................................... 15

 

2.6

 

Leak Sensor .............................................................................................................. 16

 

2.7

 

Wellness ................................................................................................................... 16

 

3

 

Installation ................................................................................................................... 17

 

3.1

 

Facility Requirements .............................................................................................. 17

 

3.2

 

Unpacking ................................................................................................................ 17

 

3.3

 

Positioning the SRD in the UltiMate 3000 System ................................................. 19

 

3.4

 

Connecting the SRD ................................................................................................ 21

 

3.4.1

 

Connecting the SRD to an UltiMate 3000 System Pump ............................... 21

 

3.4.2

 

Connecting the SRD to the Main Power Supply (Optional) .......................... 21

 

4

 

Preparation for Operation (Startup) ........................................................................ 23

 

4.1

 

Overview .................................................................................................................. 23

 

4.2

 

Connecting Drain Tubing ........................................................................................ 23

 

4.3

 

Solvent Reservoirs ................................................................................................... 24

 

4.3.1

 

General Notes ................................................................................................. 24

 

4.3.2

 

Connecting the Solvent Reservoirs ................................................................ 25

 

4.3.3

 

Connecting the Solvent Supply Lines to the Degasser ................................... 27

 

5

 

Operation and Maintenance ...................................................................................... 29

 

5.1

 

Choosing the Solvents ............................................................................................. 29

 

5.2

 

General Notes for Degasser Operation .................................................................... 31

 

5.3

 

Operational Settings ................................................................................................. 32

 

5.3.1

 

Turning the Degasser on or off (from the UltiMate 3000 System Pump) ...... 32

 

5.3.1.1

 

Turning the Degasser on and off from the Pump Display ....................... 32

 

5.3.1.2

 

Turning the Degasser on and off in Chromeleon .................................... 33

 

5.3.2

 

Turning the Degasser On or Off (Stand-alone Operation) ............................. 34

 

Summary of Contents for SRD-3200

Page 1: ...rmo Scientific Dionex UltiMate 3000 Series SRD 3x00 Solvent Racks Operating Instructions Original Operating Instructions Revision 1 8 Date September 2013 2013 Thermo Fisher Scientific Inc Doc No 4820 3550 ...

Page 2: ...UltiMate 3000 Series SRD 3x00 Solvent Racks Operating Instructions ...

Page 3: ...al safety of the products was evaluated based on the following standard DIN EN 61010 1 2010 Safety requirements for electrical equipment for measurement control and laboratory use Part 1 General Requirements The Electromagnetic Compatibility EMC of the products was evaluated based on the following standard DIN EN 61326 2006 Electrical equipment for measurement control and laboratory use EMC Requir...

Page 4: ...UltiMate 3000 Series SRD 3x00 Solvent Racks Page II Operating Instructions ...

Page 5: ... UltiMate 3000 System 19 3 4 Connecting the SRD 21 3 4 1 Connecting the SRD to an UltiMate 3000 System Pump 21 3 4 2 Connecting the SRD to the Main Power Supply Optional 21 4 Preparation for Operation Startup 23 4 1 Overview 23 4 2 Connecting Drain Tubing 23 4 3 Solvent Reservoirs 24 4 3 1 General Notes 24 4 3 2 Connecting the Solvent Reservoirs 25 4 3 3 Connecting the Solvent Supply Lines to the ...

Page 6: ...essages on the Pump Display 40 6 3 Chromeleon Diagnostics Message 40 6 4 Operating Problems 41 7 Service 45 7 1 General Notes and Safety Precautions 45 7 2 Rinsing the Degassing Channels 46 7 3 Eliminating Leakage in the Solvent Rack 47 8 Technical Information 49 9 Accessories Consumables and Spare Parts 51 9 1 Standard Accessories 51 9 2 Optional Accessories 53 9 3 Consumables and Spare Parts 53 ...

Page 7: ...uence the descriptions The software descriptions in this manual refer to Chromeleon 6 80 Service Release 13 This manual is provided as is Every effort has been made to supply complete and accurate information and all technical specifications have been developed with the utmost care The information contained in this manual should not be construed as a commitment by Thermo Fisher Scientific Thermo F...

Page 8: ...otéger l instrument contre tout dommage Label according to the Measures for Administration of the Pollution Control of Electronic Information Products China RoHS guideline Étiquette Measures for Administration of the Pollution Control of Electronic Information Products China RoHS WEEE Waste Electrical and Electronic Equipment label For more information see the WEEE Information section in the Insta...

Page 9: ...s on the device and see the related sections in these Operating Instructions Protective equipment When performing any work on or near the HPLC system wear personal protective equipment protective clothing safety gloves safety glasses as required by the hazard of the mobile phase and sample For information about the proper handling of a particular substance and for advice on specific hazards refer ...

Page 10: ...proper ventilation wear anti static clothing or shoes prevent accumulation of air bubbles in waste lines and use grounded waste containers Use only non conductive capillaries to direct solvents into the waste container With electrically conductive capillaries make sure that they are properly grounded Self ignition of solvents Do not use solvents for which the self ignition temperature is below 150...

Page 11: ...hen operating the HPLC system always set a lower pressure limit for the pump This prevents damage resulting from leakage or from running the pump dry over a longer period Activate solvent reservoir level monitoring Pump manual Use only standard HPLC grade solvents MS grade if the SRD is operated together with an NCS 3500RS or NCP 3200RS and buffers that are compatible with all parts that may be ex...

Page 12: ...écurité des substances que vous utilisez Veuillez respecter des directives des Bonnes Pratiques de Laboratoire BPL Une installation permettant de se laver les yeux ainsi qu un lavabo doivent se trouver à proximité du système Si une substance quelle qu elle soit entre en contact avec vos yeux ou votre peau rincez abondamment la zone affectée à l eau puis Substances dangereuses De nombreux solvants ...

Page 13: ...ants au réservoir de déchets Capillaires électriquement conducteur devrait être mis à la terre Inflammation spontanée des solvants N utilisez aucun solvants avec une température d auto inflammabilité inférieure à 150 C Si une fuite se produit ces solvants peuvent s auto enflammer au contact d une surface chaude Capillaires connecteur capillaires connexions ouvertes Des capillaires en particulier l...

Page 14: ... les dommages résultant de fuites ou de long terme fonctionnement à sec de la pompe Activez la surveillance de niveau liquide pour des réservoirs manuel de pompe Utilisez uniquement des solvants qualité HPLC qualité MS si vous utilisez l instrument avec un NCS 3500RS ou NCP 3200RS et des solutions salines compatibles avec les matériaux exposés phase mobiles page 49 Pour raison de sécurité ne soule...

Page 15: ...rometry Link software is required in addition to the installation of the data system Empower Installation of the Dionex Instrument Integration Software is required in addition to the installation of the data system For more information contact the Thermo Fisher Scientific sales organization for Dionex HPLC Products Operation in stand alone mode is possible as well Note that the SRD may be operated...

Page 16: ...rument n est pas utilisé involontairement 1 4 Federal Communications Commission FCC Note This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to part 15 of the U S FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates us...

Page 17: ...inless steel PEEK or titanium depending on the pump type to provide optimum resistance to the most commonly used HPLC solvents and buffer solutions 2 2 SRD Configurations The SRD is available in the following configurations SRD Description Part No SRD 3600 SRD with analytical 6 channel vacuum degasser typically for use with the following pumps one DGP 3600 two HPG 3200 SD or RS pumps in a two stac...

Page 18: ...thout degasser channels is available SR 3000 part no 5035 9200 The SR 3000 is intended only for use as a solvent organizer for example together with a LPG 3400 pump For this pump type the degasser is incorporated in the pump Tip An SRD with an analytical degasser cannot be used with a semipreparative pump ...

Page 19: ...nsor reports a leak The Status and Vacuum LEDs are red if the degasser vacuum is insufficient for proper degassing 2 Standby button Switches the SRD to standby mode The LED of the standby button is red when the SRD is in standby mode To resume operation press the standby button again the LED is not lighted Note To allow the SRD to change the mode press and hold the Standby button for at least one ...

Page 20: ...Rear panel view No Description 1 15 pin D Sub port Connects the SRD to the pump page 21 2 DC Input Power Only to be used if the SRD is not controlled by the UltiMate 3000 system pump In this case connect an external power supply unit part no 6510 0004 from here to the main power supply page 21 2 1 ...

Page 21: ...servoirs being in the tray Avertissement Pour raison de sécurité ne soulevez pas l instrument avec des réservoirs Important Do not lift the SRD by the front panel door When lifting or moving the SRD always lift by the bottom or sides of the instrument Lifting the SRD by the front panel may damage the front panel door The open front panel door is not designed to carry weight Therefore you shall not...

Page 22: ... a message is also displayed in the Chromeleon Audit Trail When the leak sensor reports a leak eliminate the cause for the leakage and dry the leak sensor page 47 2 7 Wellness System Wellness monitors the health of the SRD Therefore the SRD supports several performance and reliability features that can help you detect small problems before they turn into big ones Leak detection Status LED on the f...

Page 23: ...ble Avoid locations with extreme changes in temperature such as direct sunlight or drafts and high humidity Allow sufficient clearance behind and to the sides of the SRD for ventilation 3 2 Unpacking All electrical and mechanical components of the module are carefully tested before the instrument is shipped from the factory After unpacking inspect the instrument for any signs of mechanical damage ...

Page 24: ...s côtés Ne soulevez l instrument à l aide du matériau d emballage ou par la porte du panneau avant 3 Remove the foam spacers and then remove the polythene packaging 4 Before connecting the SRD to the power source wait approximately four hours to allow the instrument to come to room temperature and to allow any condensation that might have occurred during shipping to evaporate After four hours chec...

Page 25: ...l as shown in Fig 5 The arrangement of the system modules depends on the application Fig 4 Module arrangement for an UltiMate 3000 system example If the solvent rack is part of an UltiMate 3000 RSLCnano system or Proteomics MDLC system the related system manual UltiMate RSLCnano Standard Applications or UltiMate 3000 Proteomics MDLC System Installation and Application provides information about ho...

Page 26: ...by using the USB port on the rear panel of the instrument However Thermo Fisher Scientific recommends interconnecting all modules and then connecting the system to the Chromeleon computer with only one connection For systems with a DAD 3000 RS or MWD 3000 RS you can use only the hub on the detector for the connection For systems with a VWD 3x00 RS use only the hub on the pump Autosampler Column co...

Page 27: ...ing the SRD to the Main Power Supply Optional The SRD needs to be connected to the main power supply only if it is not operated from an UltiMate 3000 system pump For this scenario an external power supply part no 6510 0004 is available as an option for the SRD Use the external power supply unit to connect the DC input on the rear panel to a power source that is connected to a true ground No manual...

Page 28: ...UltiMate 3000 Series SRD 3x00 Solvent Racks Page 22 Operating Instructions ...

Page 29: ... the SRD with the pump of the UltiMate 3000 System Connect the pump to the degassing channels page 27 Standalone Operation Connect the degassing channels as required by your application 4 2 Connecting Drain Tubing To discharge liquid leaks that might have accumulated in the interior the SRD has a drain port at the bottom right of the instrument Fig 6 Drain port Direct liquid leaks to waste through...

Page 30: ... standard filter holders with stainless steel frits are provided in the accessories kit of the Solvent Rack Do not use these frits if the system includes a biocompatible pump and NCS 3500RS or an NCP 3200RS In this case replace the stainless steel frits with the frits from the accessories kit of the module Open the filter holder and remove the filter frit When placing the new filter frit into the ...

Page 31: ...ing guide which holds the tubing in place in the reservoir and then into the open hole in the reservoir cap 2 Verify that an appropriate frit is installed in the filter holder Pump Filter frit material porosity Part No SD N pumps Stainless steel 10 µm 6268 0110 BM pumps NCS 3500RS NCP 3200RS PEEK 10 µm 6268 0117 RS pumps AB pumps Titanium 10 µm 6268 0111 3 Slide the filter holder with filter frit ...

Page 32: ...tting plugs from the degasser inlet if necessary and connect the solvent supply line with the associated line label Fig 9 Example for degasser connections here SRD 3600 Tip The degasser ports in the upper row are combined in pairs with the related ports in the lower row Before connecting the solvent supply lines make sure that the connectors are free of contaminants Even minute particles can allow...

Page 33: ...e associated degasser outlet if necessary Fig 9 and connect the solvent supply line with the associated line label 3 Locate the solvent reservoirs as close as possible to the pump To avoid formation of air bubbles in the reservoirs and reformation of air bubbles in the solvent make sure that the reservoirs are on the same level or higher as the pump Therefore stack the SRD onto the pump as shown i...

Page 34: ...UltiMate 3000 Series SRD 3x00 Solvent Racks Page 28 Operating Instructions ...

Page 35: ...perating Instructions for the other UltiMate 3000 system modules Make sure to use special highly pure solvents They are usually labeled accordingly by the vendor Mind the special properties of the solvents such as viscosity boiling point UV absorption UV VIS detector refractive index refractive index detector and dissolved gas degasser Observe the solvent compatibility of the degasser Whenever pos...

Page 36: ...leaking or they can burst Swelling or attack by concentrated acids is not a problem with brief flushing procedures Before switching from buffer to organic solution rinse the system thoroughly with deionized water When switching to another solvent ensure that the new solvent is miscible with the one contained in the system Otherwise for example the pump can be damaged by flocculation After operatio...

Page 37: ...ng channels at regular intervals page 46 Thermo Fisher Scientific advises against recycling the solvents This may impair the degassing performance Before connecting the solvent supply lines make sure that the connectors are free of contaminants Even minute particles can allow air to enter the degasser and thus reduce the degassing effectiveness When replacing solvents make sure that the solvents a...

Page 38: ...module is operated from Chromeleon By the standby button on the front panel display page 13 Press and hold the button for one second to allow the SRD to change the mode Observe the following Turning off the module to which the SRD is connected also turns off the SRD The same applies to Standby mode Thermo Fisher Scientific recommends always leaving the degasser on 5 3 1 1 Turning the Degasser on a...

Page 39: ... N RS and BM pumps on the Devices page with an NCS NCP on the Pumps page To turn the degasser on or off from the Panel Tabset 1 In Chromeleon click the View menu and Default Panel Tabset or click the corresponding icon on the toolbar and then connect to the Chromeleon server Chromeleon creates centralized control panels called panel tabsets for all timebases available on the Chromeleon server A pa...

Page 40: ...t Command 3 Depending on the pump type click the plus sign next to PumpModule or Pump to display the items underneath 4 Set Degasser to On or Off to turn on or off the degasser Fig 12 Commands dialog box To turn the degasser on or off automatically It is also possible to include the command for turning the degasser on or off in a program file PGM for automated operation of the pump For details abo...

Page 41: ...s desired after a power failure For details see the Chromeleon Help 5 4 2 SRD Diagnostics Chromeleon 6 80 supports Diagnostics functions for the pumps of the UltiMate 3000 system also allowing you to check the performance of the solvent rack degasser For more information see the Pump and or NCS NCP manuals and the Chromeleon Help You can run the test for a SRD for which the last four digits of the...

Page 42: ...g instructions If the original shipping container is not available appropriate shipping containers and packing material can be ordered from Thermo Fisher Scientific sales organization for Dionex HPLC products The packing instructions are included in the Installation and Qualification Documents for Chromatography Instruments binder and are available on request Shipping the module in any other packa...

Page 43: ...e exact maintenance schedule for the module will depend on a number of factors Frequency What you should do Regularly To avoid contamination of the degasser Prepare fresh solvents Clean the solvent supply lines Rinse the degassing channels section 7 2 Check the fluid lines for any signs of leakage and or salt deposits Check the drain tube connected to the drain port at the bottom right of the SRD ...

Page 44: ...UltiMate 3000 Series SRD 3x00 Solvent Racks Page 38 Operating Instructions ...

Page 45: ... display of the pump Check the Messages on the Pump Display section for recommended courses of action page 40 If the pump is operated from Chromeleon a message is also displayed in the Chromeleon Audit Trail Tip For information about operating problems that might occur during the operation of an UltiMate 3000 system see Operating Problems page 41 Diagnostics Test When the SRD is operated with the ...

Page 46: ...ou are unable to eliminate the problem Message Remedial Action Degasser malfunction The vacuum level monitoring function of the degasser in the SRD was activated Turn the SRD off and on again by pressing the standby button on the front panel Contact Service if the message appears again Solvent Rack leak detected The leak sensor in the SRD has reported a leak There is a leak in the system or a flui...

Page 47: ...he UltiMate 3000 system see page 32 If the SRD is operated in standalone mode see page 34 The instrument is in standby mode Press the Standby button on the front panel An error occurred in the electronic system Contact Service The module does not work correctly when the pump is controlled by Chromeleon The SRD is not connected to the Chromeleon computer by means of a USB connection through a modul...

Page 48: ...n The eluent is degraded or of poor quality purity Use fresh solvents Use HPLC grade solvents MS grade if the SRD is operated together with an NCS 3500RS or NCP 3200RS For additional causes refer to the operating instructions for the detector Detector manual Periodic baseline fluctuation pulsation There are pressure fluctuations from the pump Purge the pump Pump manual There are air bubbles in the...

Page 49: ...on of mobile phase Select a different wavelength Use a mobile phase with less UV background absorption Poor peak area precision The capillary connections are not installed properly or they are not tight Check and tighten the capillary connections Consider using Viper capillaries Exchange the needle seat if necessary Autosampler Manual Exchange the needle if necessary Autosampler manual There are d...

Page 50: ...and the humidity are constant Consider using a thermostatted column compartment Avoid draft Contamination occurs somewhere in the system Flush the system using an appropriate solvent Check the filter frits in the solvent supply line filters for contamination Replace the frits as necessary For additional causes refer to the operating instructions for the autosampler and for the pump Autosampler man...

Page 51: ...omposés et des recommandations pour les situations de risque spécifiques veuillez consulter la fiche de données de sécurité des substances que vous utilisez Veuillez respecter des directives des Bonnes Pratiques de Laboratoire BPL Observe the following For all service and repair procedures observe all precautionary statements provided in these operating instructions Use only the original spare par...

Page 52: ...ble ghost peaks in the chromatogram follow these steps 1 Install a backpressure capillary on the pump outlet The capillary should be appropriate for generating a backpressure of 20 to 30 MPa 2 Rinse the degassing channels for 1 hour with 20 nitric acid at the flow rate normally used for your application 3 Rinse the degassing channels with fresh HPLC grade water MS grade if the SRD is operated toge...

Page 53: ...eaking connections if necessary 2 Dry the components 3 With a cloth or tissue absorb all liquid that has collected in the tray Make sure that you do not bend or damage the sensor Fig 13 Drying the leak sensor If the SRD is operated from the pump of the UltiMate 3000 system the message remains on the pump display for information purposes even after the source for the leak has been eliminated Select...

Page 54: ...UltiMate 3000 Series SRD 3x00 Solvent Racks Page 48 Operating Instructions ...

Page 55: ... standalone operation Communication 15 pin D Sub connector through the pump of the UltiMate 3000 system Power supply 15 pin D Sub connector through the pump of the UltiMate 3000 system or external power supply unit optional User input display 3 LEDs Power Vacuum and Status Standby button Wetted parts Amorphous fluoropolymer AF PEEK FEP and EFTC ECTFE Solvent supply line filter frit SST PEEK or tit...

Page 56: ...UltiMate 3000 Series SRD 3x00 Solvent Racks Page 50 Operating Instructions ...

Page 57: ...mation contact the Thermo Fisher Scientific sales organization for Dionex HPLC Products Description Quantity in the kit Part No SRD 3600 accessories including Cable connecting the SRD and the UltiMate 3000 system module pump NCS 3500RS or NCP 3200RS 1 6000 1006 Cap to close the holes in the solvent bottle caps 40 Included in 6000 0047 Bottle cap for solvent reservoirs including caps to close the h...

Page 58: ... Included in 6000 0042 1 liter solvent reservoir 4 2270 0012 Solvent supply line analytical 4 6030 2548 Tubing labels white A B C D 2 each Description Quantity in the kit Part No SRD 3200 accessories including Cable connecting the SRD and the UltiMate 3000 system module pump NCS 3500RS or NCP 3200RS 1 6000 1006 Cap to close the holes in the solvent bottle caps 20 Included in 6000 0047 Bottle cap f...

Page 59: ...lter Filter frit stainless steel porosity 10 µm pack of 10 frits for SD N pumps Filter frit PEEK porosity 10 µm pack of 10 frits for BM pumps NCS 3500RS and NCP 3200RS Filter frit titanium porosity 10 µm pack of 10 frits for RS and AB pumps 6268 0110 6828 0117 6828 0111 Solvent reservoir 1 L with cap 2270 0012 External power supply unit 6510 0004 Bottle cap for solvent reservoirs including caps to...

Page 60: ...UltiMate 3000 Series SRD 3x00 Solvent Racks Page 54 Operating Instructions ...

Page 61: ...as Ground_supply Reference potential for V_Degas 8 VCC Voltage for logic devices 9 Jumper to pin 3 10 GND Reference potential for VCC 11 GND Reference potential for VCC 12 GND Reference potential for VCC 13 Reserved 14 V_Degas 24V_supply Supply for the SRD 15 GND_Degas Ground_supply Reference potential for V_Degas Fig 14 15 pin D Sub port male 10 2 DC Input The DC input is used to connect the SRD ...

Page 62: ...UltiMate 3000 Series SRD 3x00 Solvent Racks Page 56 Operating Instructions ...

Page 63: ... Connections 15 Pump 27 Solvent Reservoirs 25 I Installation 17 Intended Use 9 L Leak 35 Leak Monitoring 35 Leak Sensor 16 47 M Maintenance 37 Maintenance Intervals 37 Messages 40 Module Display 13 O Operating Instructions 1 Operating Problems 41 Operation 29 After Power Failure 35 Control Elements 13 Degasser 31 Display 13 from Chromeleon 32 from UltiMate 3000 System 32 Safety Precautions 3 Setti...

Page 64: ...ak 35 Solvent Reservoirs 24 Connect 24 General Notes 24 Spare Parts 53 Specification 49 SRD Drainage 23 Standalone Operation 34 Status Screen 13 Symbols 2 T Troubleshooting 39 Diagnostics Messages 40 Display Messages 40 Operating Problems 41 U Unit Description Overview 11 Unpacking 17 V Vacuum 35 Vacuum Degasser Solvent Compatibility 29 Vacuum Monitoring 35 W Wellness 16 ...

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