Thermo Scientific SRD-3200, Operating Instructions Manual

Page: 50 / 64

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Page 44 Operating Instructions
Problem Probable Cause Remedial Action
Poor peak area precision
(Cont'd)
The gradient is irreproducible. RS, SD(N), and BM pumps:
-
Change the gradient.
- Check the pump function and
degassing.
- Check the filter frits in the
solvent supply line filters for
contamination. Replace the frits
as necessary.
NCS-3500RS, NCP-3200RS:
The pressure transducer offset
may not be correct. Check the
offset and calibrate the pressure
transducers if required
( →
NCS/NCP manual
).
The environmental conditions are
unstable.
Make sure that the temperature
and the humidity are constant.
Consider using a thermostatted
column compartment. Avoid
draft.
Contamination occurs somewhere
in the system.
Flush the system using an
appropriate solvent. Check the
filter frits in the solvent supply
line filters for contamination.
Replace the frits as necessary.
For additional causes, refer to the
operating instructions for the
autosampler and for the pump.
→ Autosampler manual
→
Pump manual
→ NCS/NCP manual
Poor degassing There is a leak in the capillaries or
solvent supply lines.
Inspect the capillaries and
solvent supply lines for
leakage; tighten loose fitting
connections.
The flow rate is too high. Reduce the flow rate.
Degasser noise The vacuum pump of the degasser
is running at high speed.
Fill and degas all channels
(even if they are not used for
the application).