Thermo Scientific SRD-3200, Operating Instructions Manual

Page: 48 / 64

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Page 42 Operating Instructions
Problem Probable Cause Remedial Action
High baseline drift
(Cont'd)
The eluents are degraded or
inhomogeneous.
Before you start an analysis, be
sure that the eluents are already
homogenized in the reservoirs.
Use fresh solvent and check the
eluent filter frits. In aqueous
solvents, growth of
microorganisms is possible.
The environmental conditions are
unstable.
Make sure that the temperature
and the humidity are constant.
Avoid draft. Verify on the
detector that the lamp and flow
cell covers are in proper
position and that the front panel
door is closed.
The mobile phase is delivered in
circles.
Direct the mobile phase to
waste.
For additional causes, refer to the
operating instructions for the
detector.
→
Detector manual
High noise level, non-
periodic baseline
fluctuation
The eluent is degraded or of poor
quality/purity.
Use fresh solvents. Use HPLC
grade solvents (MS grade if the
SRD is operated together with
an NCS-3500RS or NCP-
3200RS).
For additional causes, refer to the
operating instructions for the
detector.
→ Detector manual
Periodic baseline
fluctuation, pulsation
There are pressure fluctuations
from the pump.
Purge the pump
( → Pump manual ).
There are air bubbles in the system. Purge the pump
( →
Pump manual
).
Reproducible ghost peaks
in the chromatogram.
The degassing channels are
contaminated.
Rinse the degassing channels
( → page 46).
The eluents are degraded, dirty or
of poor purity/quality.
Use fresh eluent. Use HPLC
grade solvents (MS grade if the
SRD is operated together with
an NCS-3500RS or NCP-
3200RS).
Contamination occurs somewhere
in the system.
Flush the system using an
appropriate solvent.
Additional peaks appear
in the injection peak.
With gradients, the equilibration
time after the flush cycle is too
short.
Extend the equilibration time.
There is excessive dead volume. Eliminate possibly existing
dead volume.