Thermo Scientific Shake 'n Stack 6244, Operating And Maintenance Manual

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© Thermo
Scientific
, May 2003. Issue 7 13
5. Place 10-15mls SSPE (SSC) into a Hybridisation Bottle and then insert the roll in
such a way that the leading edge (inside the roll) and the trailing edge are
positioned relative to each other as shown in Figure 3.2).
6. Place the bottle on a flat surface and then slowly unwind the membrane and mesh
around the inside of the bottle by rocking and gently rolling the bottle along the
surface. No air bubbles should be visible between the membrane and the bottle. If
bubbles are present, the membrane should be removed and re-rolled.
The procedure should then be repeated more gently. Rock the bottle backwards
and forwards to attach the first part of the membrane to the bottle. Then roll the
bottle in order to unwind the mesh and membrane.
7. Continue until the membrane and mesh is fully unwound. The mesh ensures the
probe will have access to all parts of the Hybridisation membrane including those
parts of the membrane, which overlap.